TY  - JOUR
A1  - Yarman, Aysu
A1  - Badalyan, Artavazd
A1  - Gajovic-Eichelmann, Nenad
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Enzyme electrode for aromatic compounds exploiting the catalytic activities of microperoxidase-11
JF  - Biosensors and bioelectronics : the principal international journal devoted to research, design development and application of biosensors and bioelectronics
N2  - Microperoxidase-11 (MR-11) which has been immobilised in a matrix of chitosan-embedded gold nanoparticles on the surface of a glassy carbon electrode catalyzes the conversion of aromatic substances. This peroxide-dependent catalysis of microperoxidase has been applied in an enzyme electrode for the first time to indicate aromatic compounds such as aniline. 4-fluoroaniline, catechol and p-aminophenol. The electrode signal is generated by the cathodic reduction of the quinone or quinoneimine which is formed in the presence of both MP-II and peroxide from the substrate. The same sensor principle will be extended to aromatic drugs.
KW  - Microperoxidase-11
KW  - Nanoparticles
KW  - p-Aminophenol
KW  - Aniline
KW  - Catechol
KW  - 4-Fluoroaniline
KW  - Biosensors
Y1  - 2011
U6  - https://doi.org/10.1016/j.bios.2011.09.004
SN  - 0956-5663
VL  - 30
IS  - 1
SP  - 320
EP  - 323
PB  - Elsevier
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Yarman, Aysu
A1  - Nagel, Thomas
A1  - Gajovic-Eichelmann, Nenad
A1  - Fischer, Anna
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Bioelectrocatalysis by Microperoxidase-11 in a Multilayer Architecture of Chitosan Embedded Gold Nanoparticles
JF  - Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis
N2  - We report on the redox behaviour of the microperoxidase-11 (MP-11) which has been electrostatically immobilized in a matrix of chitosan-embedded gold nanoparticles on the surface of a glassy carbon electrode. MP-11 contains a covalently bound heme c as the redox active group that exchanges electrons with the electrode via the gold nanoparticles. Electroactive surface concentration of MP-11 at high scan rate is between 350+/-50 pmol cm(-2), which reflects a multilayer process. The formal potential (E degrees') of MP-11 in the gold nanoparticles-chitosan film was estimated to be -(267.7+/-2.9) mV at pH 7.0. The heterogeneous electron transfer rate constant (k(s)) starts at 1.21 s(-1) and levels off at 6.45 s(-1) in the scan rate range from 0.1 to 2.0 V s(-1). Oxidation and reduction of MP-11 by hydrogen peroxide and superoxide, respectively have been coupled to the direct electron transfer of MP-11.
KW  - Microperoxidase
KW  - Direct electron transfer
KW  - Nanoparticles
KW  - Hydrogen peroxide
KW  - Superoxide
KW  - Bioelectrocatalysis
Y1  - 2011
U6  - https://doi.org/10.1002/elan.201000535
SN  - 1040-0397
VL  - 23
IS  - 3
SP  - 611
EP  - 618
PB  - Wiley-Blackwell
CY  - Malden
ER  - 
TY  - JOUR
A1  - Zeng, Ting
A1  - Frasca, Stefano
A1  - Rumschöttel, Jens
A1  - Koetz, Joachim
A1  - Leimkühler, Silke
A1  - Wollenberger, Ursula
T1  - Role of Conductive Nanoparticles in the Direct Unmediated Bioelectrocatalysis of Immobilized Sulfite Oxidase
JF  - Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis
KW  - Direct electron transfer
KW  - Protein voltammetry
KW  - Human sulfite oxidase
KW  - Bioelectrocatalysis
KW  - Nanoparticles
Y1  - 2016
U6  - https://doi.org/10.1002/elan.201600246
SN  - 1040-0397
SN  - 1521-4109
VL  - 28
SP  - 2303
EP  - 2310
PB  - Wiley-VCH
CY  - Weinheim
ER  -