TY  - JOUR
A1  - Singh, Jasbir
A1  - Singh, S.
A1  - Dani, H. M.
A1  - Sharma, Reeta
A1  - Steinberg, Pablo
T1  - Interactions of aflatoxin B-1 with SRP components can disrupt protein targeting
N2  - Spectrofluorimetric studies have revealed that aflatoxin B-1 (AFB(1)) interacts with signal recognition particle (SRP), which acts as an escort for polyribosomes with signal peptides to be transported and bound to the cytoplasmic face of the endoplasmic reticulum (ER). We further report that the binding of AFB(1) to SRP is selective as it only binds to two (SRP9 and 14) out of its three constituent polypeptides studied. Binding of AFB(1) to proteins is known to alter their conformations. Interactions of AFB(1) with SRP polypeptides may generate structural and functional alterations in this particle and hinder secretory protein synthesis. Copyright (C) 2004 John Wiley Sons, Ltd
Y1  - 2005
SN  - 0263-6484
ER  - 
TY  - JOUR
A1  - Thierbach, Renè
A1  - Schulz, Tim Julius
A1  - Isken, Frank
A1  - Voigt, Aanja
A1  - Mietzner, Brun
A1  - Drewes, Gunnar
A1  - von Kleist-Retzow, Jürgen-Christoph
A1  - Wiesner, Rudolf J.
A1  - Magnuson, Mark A.
A1  - Puccio, Helene
A1  - Pfeiffer, Andreas F. H.
A1  - Steinberg, Pablo
A1  - Ristow, Michael
T1  - Targeted disruption of hepatic frataxin expression causes impaired mitochondrial function, decreased life span and tumor growth in mice
N2  - We have disrupted expression of the mitochondrial Friedreich ataxia protein frataxin specifically in murine hepatocytes to generate mice with impaired mitochondrial function and decreased oxidative phosphorylation. These animals have a reduced life span and develop multiple hepatic tumors. Livers also show increased oxidative stress, impaired respiration and reduced ATP levels paralleled by reduced activity of iron-sulfur cluster (Fe/S) containing proteins (ISP), which all leads to increased hepatocyte turnover by promoting both apoptosis and proliferation. Accordingly, phosphorylation of the stress-inducible p38 MAP kinase was found to be specifically impaired following disruption of frataxin. Taken together, these findings indicate that frataxin may act as a mitochondrial tumor suppressor protein in mammals
Y1  - 2005
ER  - 
TY  - JOUR
A1  - Fuchs, J.
A1  - Teubner, Wera
A1  - Steinberg, Pablo
T1  - The resistance of intestinal epithelial cells towards the transforming activity of 2-hydroxyamino-1-methyl-6- phenylimidazo[4,5-B]pyridine is accompanied by glutathione S-transferase induction
Y1  - 2004
SN  - 0028-1298
ER  - 
TY  - JOUR
A1  - Teubner, Wera
A1  - Langheinrich, C.
A1  - Seidel, Albrecht
A1  - Steinberg, Pablo
T1  - Inhibition of p53 transactivation activity does not promote mutagen-induced transformation of IEC-18
Y1  - 2004
SN  - 0028-1298
ER  - 
TY  - JOUR
A1  - Kühnel, Dana
A1  - Steinberg, Pablo
A1  - Scholtka, Bettina
T1  - A human-relevant dose of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PHIP) can induce precancerous lesions in rat intestine after 6 months of exposure
Y1  - 2004
SN  - 0028-1298
ER  - 
TY  - JOUR
A1  - Thierbach, Rene
A1  - Schulz, Tim Julius
A1  - Voigt, Aanja
A1  - Drewes, Gunnar
A1  - Isken, F.
A1  - Pfeiffer, Andreas F. H.
A1  - Ristow, Michael
A1  - Steinberg, Pablo
T1  - Targeted disruption of frataxin in hepatocytes causes spontaneous neoplasia accompanied by increased ROS formation
Y1  - 2004
SN  - 0028-1298
ER  - 
TY  - JOUR
A1  - Herbst, Uta
A1  - Fuchs, Iris Judith
A1  - Teubner, Wera
A1  - Seidel, Albrecht
A1  - Frank, Heinz
A1  - Steinberg, Pablo
T1  - Malignant transformation of human colon epithelial cells by polycyclic aromatic hydrocarbons and heterocyclic aromatic amines
Y1  - 2004
SN  - 0028-1298
ER  - 
TY  - JOUR
A1  - Stark, Avishay abraham
A1  - Porat, Noga
A1  - Volohonsky, Gloria
A1  - Konlosh, A.
A1  - Bluvshtein, Evgenia
A1  - Tubi, C.
A1  - Steinberg, Pablo
T1  - The role of gamma-glutamyl transpeptidase in the biosynthesis of glutathione
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Okano, J.
A1  - Shiota, G.
A1  - Matsumoto, K.
A1  - Yasui, S.
A1  - Kurimasa, A.
A1  - Hisatome, I.
A1  - Steinberg, Pablo
A1  - Murawaki, Y.
T1  - Hepatocyte growth factor exerts a proliferative effect on oval cells through the PI3K/AKT signaling pathway
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Bartsch, Ingrid
A1  - Zschaler, Ingrid
A1  - Haseloff, Monika
A1  - Steinberg, Pablo
T1  - Establishment of a long-term culture system for rat colon epithelial cells
N2  - The aim of this study was to establish a long-term culture. system for rat colon epithelia isolaled by incubating a 4-cm-long rat colon segment cut longitudinally with all ethylenediaminetetraacetic acid [disodium salt]- containing buffer, taken up in conditioned medium from the normal rat kidney fibroblast cell line NRK (i.e., the supernatant Of pure NRK cultures), directly plated on mitomycin C-treated NRK cells and subcultured with conditioned medium from NRK cells. Cells started to migrate out of the crypts shortly after plating them on NRK feeder layers. Some of the crypts fell apart during the isolation procedure. whereas the vast majority of them did it within I to 2 Ill after plating. The cells proliferated extremely slowly but continuously over a period of 4 mo and were epithelial because they expressed cytokeratin 19 and were stained by crystal violet at pH 2.8. In conclusion, the experimental system described ill this study allows to maintain rat colon epithelial cells for up to 4 mo in culture and can be used to Study the effects of a variety of tumor-modulating factors on growth and gene expression of normal colon epithelial cells in vitro
Y1  - 2003
UR  - http://www.springerlink.com/content/120498/
U6  - https://doi.org/10.1290/0404035.1
SN  - 1071-2690
ER  -