TY - JOUR A1 - Sarauli, David A1 - Peters, Kristina A1 - Xu, Chenggang A1 - Schulz, Burkhard A1 - Fattakhova-Rohlfing, Dina A1 - Lisdat, Fred T1 - 3D-Electrode architectures for enhanced direct bioelectrocatalysis of pyrroloquinoline quinone-dependent glucose dehydrogenase JF - ACS applied materials & interfaces N2 - We report on the fabrication of a complex electrode architecture for efficient direct bioelectrocatalysis. In the developed procedure, the redox enzyme pyrroloquinoline quinone-dependent glucose dehydrogenase entrapped in a sulfonated polyaniline [poly(2-methoxyaniline-5-sulfonic acid)-co-aniline] was immobilized on macroporous indium tin oxide (macroITO) electrodes. The use of the 3D-conducting scaffold with a large surface area in combination with the conductive polymer enables immobilization of large amounts of enzyme and its efficient communication with the electrode, leading to enhanced direct bioelectrocatalysis. In the presence of glucose, the fabricated bioelectrodes show an exceptionally high direct bioelectrocatalytical response without any additional mediator. The catalytic current is increased more than 200-fold compared to planar ITO electrodes. Together with a high long-term stability (the current response is maintained for >90% of the initial value even after 2 weeks of storage), the transparent 3D macroITO structure with a conductive polymer represents a valuable basis for the construction of highly efficient bioelectronic units, which are useful as indicators for processes liberating glucose and allowing optical and electrochemical transduction. KW - 3D electrode structures KW - macroITO KW - conductive polymer KW - PQQ-GDH KW - direct bioelectrocatalysis KW - bioelectrochemistry Y1 - 2014 U6 - https://doi.org/10.1021/am5046026 SN - 1944-8244 VL - 6 IS - 20 SP - 17887 EP - 17893 PB - American Chemical Society CY - Washington ER -