TY  - JOUR
A1  - Heilmann, Katja
A1  - Groth, Thomas
A1  - Schossig, Michael
A1  - Lendlein, Andreas
A1  - Micheel, Burkhard
T1  - Modulation of hybridoma cell growth and antibody production by coating cell culture material with extracellular matrix proteins
N2  - The influence of coating polystyrene tissue culture plates with different proteins on murine hybridoma cell growth and antibody production was investigated. Fibronectin, collagen I, bovine serum albumin and laminin were used to coat NUNC and COSTAR cell culture plates. Cell number and antibody concentration in culture fluids were quantified as indicators for cell viability, proliferation and productivity. Adhesive behaviour, morphology, expression of surface receptors of hybridoma cells and the presence of tyrosine-phosphorylated proteins in cell lysates were characterized by cell adhesion experiments, microscopy, flow cytometry and Western Blot analysis. It was shown that coatings with fibronectin (0.2 ;g/ml) lead to a substantial improvement of cell growth by 50-70% and an increase of monoclonal antibody production by 100-120%. Collagen I coatings showed an improvement in cell growth by 30-70% and by 60% for the production of monoclonal antibodies. Coatings with BSA and laminin had minor effects on these parameters. It was found that the hybridoma cell lines used in this study did not express the ;2-chain of the ;2;1-integrin, which is responsible for binding to collagen and laminin. However, the presence of ;1- integrin on the cell surface was shown, which should enable hybridoma cells to bind fibronectin. We propose, therefore, that fibronectin adsorption to cell culture materials may be a promising approach to enhance the production of monoclonal antibodies by cultivated hybridoma cells.
Y1  - 2007
UR  - http://www.sciencedirect.com/science/journal/1369703X
U6  - https://doi.org/10.1016/j.bej.2007.01.035
SN  - 1369-703X
ER  - 
TY  - JOUR
A1  - Sauter, Tilman
A1  - Lützow, Karola
A1  - Schossig, Michael
A1  - Kosmella, Hans
A1  - Weigel, Thomas
A1  - Kratz, Karl
A1  - Lendlein, Andreas
T1  - Shape-memory properties of polyetherurethane foams prepared by thermally induced phase separation
JF  - Advanced engineering materials
N2  - In this study, we report the preparation of two structurally different shape-memory polymer foams by thermally induced phase separation (TIPS) from amorphous polyetherurethanes. Foams with either a homogeneous, monomodal, or with a hierarchically structured, bimodal, pore size distribution are obtained by adoption of the cooling protocol. The shape-memory properties have been investigated for both foam structures by cyclic, thermomechanical experiments, while the morphological changes on the micro scale (pore level) have been compared to the macro scale by an in situ micro compression device experiment. The results show that the hierarchically structured foam achieves higher shape-recovery rates and a higher total recovery as compared to the homogeneous foam, which is due to an increased energy storage capability by micro scale bending of the hierarchically structured foam compared to pure compression of the homogeneous foam.
Y1  - 2012
U6  - https://doi.org/10.1002/adem.201200127
SN  - 1438-1656
VL  - 14
IS  - 9
SP  - 818
EP  - 824
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Sauter, Tilman
A1  - Lützow, Karola
A1  - Schossig, Michael
A1  - Kosmella, Hans
A1  - Weigel, Thomas
A1  - Kratz, Karl
A1  - Lendlein, Andreas
T1  - Pore morphology as structural parameter to tailor the shape-memory effect of polyuetherurethane foams
T2  - Abstracts of papers : joint conference / The Chemical Institute of Cananda, CIC, American Chemical Society, ACS
Y1  - 2013
SN  - 0065-7727
VL  - 245
PB  - American Chemical Society
CY  - Washington
ER  -