TY  - JOUR
A1  - Schippers, Jos H. M.
A1  - Nguyen, Hung M.
A1  - Lu, Dandan
A1  - Schmidt, Romy
A1  - Müller-Röber, Bernd
T1  - ROS homeostasis during development: an evolutionary conserved strategy
JF  - Cellular and molecular life sciences
N2  - The balance between cellular proliferation and differentiation is a key aspect of development in multicellular organisms. Recent studies on Arabidopsis roots revealed distinct roles for different reactive oxygen species (ROS) in these processes. Modulation of the balance between ROS in proliferating cells and elongating cells is controlled at least in part at the transcriptional level. The effect of ROS on proliferation and differentiation is not specific for plants but appears to be conserved between prokaryotic and eukaryotic life forms. The ways in which ROS is received and how it affects cellular functioning is discussed from an evolutionary point of view. The different redox-sensing mechanisms that evolved ultimately result in the activation of gene regulatory networks that control cellular fate and decision-making. This review highlights the potential common origin of ROS sensing, indicating that organisms evolved similar strategies for utilizing ROS during development, and discusses ROS as an ancient universal developmental regulator.
KW  - Evolution
KW  - Reactive oxygen species
KW  - Development
Y1  - 2012
U6  - https://doi.org/10.1007/s00018-012-1092-4
SN  - 1420-682X
VL  - 69
IS  - 19
SP  - 3245
EP  - 3257
PB  - Springer
CY  - Basel
ER  - 
TY  - JOUR
A1  - Schmidt, Romy
A1  - Schippers, Jos H. M.
A1  - Welker, Annelie
A1  - Mieulet, Delphine
A1  - Guiderdoni, Emmanuel
A1  - Müller-Röber, Bernd
T1  - Transcription factor OsHsfC1b regulates salt tolerance and development in Oryza sativa ssp japonica
JF  - AoB PLANTS
N2  - Background and aims Salt stress leads to attenuated growth and productivity in rice. Transcription factors like heat shock factors (HSFs) represent central regulators of stress adaptation. Heat shock factors of the classes A and B are well established as regulators of thermal and non-thermal stress responses in plants; however, the role of class C HSFs is unknown. Here we characterized the function of the OsHsfC1b (Os01g53220) transcription factor from rice.
 Methodology We analysed the expression of OsHsfC1b in the rice japonica cultivars Dongjin and Nipponbare exposed to salt stress as well as after mannitol, abscisic acid (ABA) and H2O2 treatment. For functional characterization of OsHsfC1b, we analysed the physiological response of a T-DNA insertion line (hsfc1b) and two artificial micro-RNA (amiRNA) knock-down lines to salt, mannitol and ABA treatment. In addition, we quantified the expression of small Heat Shock Protein (sHSP) genes and those related to signalling and ion homeostasis by quantitative real-time polymerase chain reaction in roots exposed to salt. The subcellular localization of OsHsfC1b protein fused to green fluorescent protein (GFP) was determined in Arabidopsis mesophyll cell protoplasts.
 Principal results Expression of OsHsfC1b was induced by salt, mannitol and ABA, but not by H2O2. Impaired function of OsHsfC1b in the hsfc1b mutant and the amiRNA lines led to decreased salt and osmotic stress tolerance, increased sensitivity to ABA, and temporal misregulation of salt-responsive genes involved in signalling and ion homeostasis. Furthermore, sHSP genes showed enhanced expression in knock-down plants under salt stress. We observed retarded growth of hsfc1b and knock-down lines in comparison with control plants under non-stress conditions. Transient expression of OsHsfC1b fused to GFP in protoplasts revealed nuclear localization of the transcription factor.
 Conclusions OsHsfC1b plays a role in ABA-mediated salt stress tolerance in rice. Furthermore, OsHsfC1b is involved in the response to osmotic stress and is required for plant growth under non-stress conditions.
Y1  - 2012
U6  - https://doi.org/10.1093/aobpla/pls011
SN  - 2041-2851
IS  - 3
PB  - Oxford Univ. Press
CY  - Oxford
ER  -