TY  - JOUR
A1  - French, Alice S.
A1  - Simcock, Kerry L.
A1  - Rolke, Daniel
A1  - Gartside, Sarah E.
A1  - Blenau, Wolfgang
A1  - Wright, Geraldine A.
T1  - The role of serotonin in feeding and gut contractions in the honeybee
JF  - Journal of insect physiology
KW  - Honeybee
KW  - Apis mellifera
KW  - Serotonin
KW  - 5-HT
KW  - 5-HT receptor
KW  - Gut contractions
Y1  - 2014
U6  - https://doi.org/10.1016/j.jinsphys.2013.12.005
SN  - 0022-1910
SN  - 1879-1611
VL  - 61
SP  - 8
EP  - 15
PB  - Elsevier
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Reim, Tina
A1  - Thamm, Markus
A1  - Rolke, Daniel
A1  - Blenau, Wolfgang
A1  - Scheiner, Ricarda
T1  - Suitability of three common reference genes for quantitative real-time PCR in honey bees
JF  - Apidologie : a quality journal in bee science
N2  - Honey bees are important model organisms for neurobiology, because they display a large array of behaviors. To link behavior with individual gene function, quantitative polymerase chain reaction is frequently used. Comparing gene expression of different individuals requires data normalization using adequate reference genes. These should ideally be expressed stably throughout lifetime. Unfortunately, this is frequently not the case. We studied how well three commonly used reference genes are suited for this purpose and measured gene expression in the brains of honey bees differing in age and social role. Although rpl32 is used most frequently, it only remains stable in expression between newly emerged bees, nurse-aged bees, and pollen foragers but shows a peak at the age of 12 days. The genes gapdh and ef1 alpha-f1, in contrast, are expressed stably in the brain throughout all age groups except newly emerged bees. According to stability software, gapdh was expressed most stably, followed by rpl32 and ef1 alpha-f1.
KW  - gene expression
KW  - quantitative PCR
KW  - reference gene
KW  - stability program
KW  - Apis mellifera
Y1  - 2013
U6  - https://doi.org/10.1007/s13592-012-0184-3
SN  - 0044-8435
VL  - 44
IS  - 3
SP  - 342
EP  - 350
PB  - Springer
CY  - Paris
ER  -