TY  - JOUR
A1  - Richter, Katharina Natalia
A1  - Rolke, Daniel
A1  - Blenau, Wolfgang
A1  - Baumann, Otto
T1  - Secretory cells in honeybee hypopharyngeal gland: polarized organization and age-dependent dynamics of plasma membrane
JF  - Cell & tissue research
N2  - The honeybee hypopharyngeal gland consists in numerous units, each comprising a secretory cell and a canal cell. The secretory cell discharges its products into a convoluted tubular membrane system, the canaliculus, which is surrounded at regular intervals by rings of actin filaments. Using probes for various membrane components, we analyze the organization of the secretory cells relative to the apicobasal configuration of epithelial cells. The canaliculus was defined by labeling with an antibody against phosphorylated ezrin/radixin/moesin (pERM), a marker protein for the apical membrane domain of epithelial cells. Anti-phosphotyrosine visualizes the canalicular system, possibly by staining the microvillar tips. The open end of the canaliculus leads to a region in which the secretory cell is attached to the canal cell by adherens and septate junctions. The remaining plasma membrane stains for Na,K-ATPase and spectrin and represents the basolateral domain. We also used fluorophore-tagged phalloidin, anti-phosphotyrosine and anti-pERM as probes for the canaliculus in order to describe fine-structural changes in the organization of the canalicular system during the adult life cycle. These probes in conjunction with fluorescence microscopy allow the fast and detailed three-dimensional analysis of the canalicular membrane system and its structural changes in a developmental mode or in response to environmental factors.
KW  - Hypopharyngeal gland
KW  - Cell polarity
KW  - Moesin
KW  - Actin cytoskeleton
KW  - Honeybee
Y1  - 2016
U6  - https://doi.org/10.1007/s00441-016-2423-9
SN  - 0302-766X
SN  - 1432-0878
VL  - 366
SP  - 163
EP  - 174
PB  - Springer
CY  - New York
ER  -