TY  - JOUR
A1  - Prokopovic, Vladimir Z.
A1  - Duschl, Claus
A1  - Volodkin, Dmitry
T1  - Hyaluronic Acid/Poly-l-Lysine Multilayers as Reservoirs for Storage and Release of Small Charged Molecules
JF  - Macromolecular bioscience
N2  - Polyelectrolyte multilayer films are nowadays very attractive for bioapplications due to their tunable properties and ability to control cellular response. Here we demonstrate that multilayers made of hyaluronic acid and poly-l-lysine act as high-capacity reservoirs for small charged molecules. Strong accumulation within the film is explained by electrostatically driven binding to free charges of polyelectrolytes. Binding and release mechanisms are discussed based on charge balance and polymer dynamics in the film. Our results show that transport of molecules through the film-solution interface limits the release rate. The multilayers might serve as an effective platform for drug delivery and tissue engineering due to high potential for drug loading and controlled release.
KW  - diffusion
KW  - drug delivery
KW  - dye
KW  - release mechanism
Y1  - 2015
U6  - https://doi.org/10.1002/mabi.201500093
SN  - 1616-5187
SN  - 1616-5195
VL  - 15
IS  - 10
SP  - 1357
EP  - 1363
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Prokopovic, Vladimir Z.
A1  - Vikulina, Anna S.
A1  - Sustr, David
A1  - Duschl, Claus
A1  - Volodkin, Dmitry
T1  - Biodegradation-Resistant Multilayers Coated with Gold Nanoparticles. Toward a Tailor-made Artificial Extracellular Matrix
JF  - Journal of colloid and interface science
N2  - Polymer multicomponent coatings such as multilayers mimic an extracellular, matrix (ECM) that attracts significant attention for the use of the multilayers as functional supports for advanced cell culture and tissue engineering. Herein, biodegradation and molecular transport in hyaluronan/polylysine multilayers coated with gold nanoparticles were described. Nanoparticle coating acts as a semipermeable barrier that governs molecular transport into/from the multilayers, and makes them biodegradation-resistant. Model protein lysozyme (mimics of ECM-soluble signals) diffuses into the multilayers as fast- and, slow-diffusing populations existing in an equilibrium,. Such a. composite system may have high potential to be exploited as degradation-resistant drug-delivery platforms suitable for cell-based applications.
KW  - hyaluronic acid
KW  - polylysine
KW  - diffusion
KW  - semipermeable
KW  - fluorescence recovery after photobleaching
KW  - layer-by-layer
KW  - enzymatic degradation
KW  - cell adhesion
Y1  - 2016
U6  - https://doi.org/10.1021/acsami.6b10095
SN  - 1944-8244
VL  - 8
SP  - 24345
EP  - 24349
PB  - American Chemical Society
CY  - Washington
ER  -