TY  - JOUR
A1  - Kiefer, Christian S.
A1  - Claes, Andrea R.
A1  - Nzayisenga, Jean-Claude
A1  - Pietra, Stefano
A1  - Stanislas, Thomas
A1  - Ikeda, Yoshihisa
A1  - Grebe, Markus
T1  - Arabidopsis AIP1-2 restricted by WER-mediated patterning modulates planar polarity
JF  - Development
N2  - The coordination of cell polarity within the plane of the tissue layer (planar polarity) is crucial for the development of diverse multicellular organisms. Small Rac/Rho-family GTPases and the actin cytoskeleton contribute to planar polarity formation at sites of polarity establishment in animals and plants. Yet, upstream pathways coordinating planar polarity differ strikingly between kingdoms. In the root of Arabidopsis thaliana, a concentration gradient of the phytohormone auxin coordinates polar recruitment of Rho-of-plant (ROP) to sites of polar epidermal hair initiation. However, little is known about cytoskeletal components and interactions that contribute to this planar polarity or about their relation to the patterning machinery. Here, we show that ACTIN7 (ACT7) represents a main actin isoform required for planar polarity of root hair positioning, interacting with the negative modulator ACTIN-INTERACTING PROTEIN1-2 (AIP1-2). ACT7, AIP1-2 and their genetic interaction are required for coordinated planar polarity of ROP downstream of ethylene signalling. Strikingly, AIP1-2 displays hair cell file-enriched expression, restricted by WEREWOLF (WER)-dependent patterning and modified by ethylene and auxin action. Hence, our findings reveal AIP1-2, expressed under control of the WER-dependent patterning machinery and the ethylene signalling pathway, as a modulator of actin-mediated planar polarity.
KW  - AIP1
KW  - Actin
KW  - Arabidopsis
KW  - Patterning
KW  - Planar polarity
Y1  - 2015
UR  - http://dev.biologists.org/content/142/1/151.long
U6  - https://doi.org/doi: 10.1242/dev.111013
IS  - 142
SP  - 151
EP  - 161
ER  - 
TY  - JOUR
A1  - Kiefer, Christian S.
A1  - Claes, Andrea R.
A1  - Nzayisenga, Jean-Claude
A1  - Pietra, Stefano
A1  - Stanislas, Thomas
A1  - Hueser, Anke
A1  - Ikeda, Yoshihisa
A1  - Grebe, Markus
T1  - Arabidopsis AIP1-2 restricted by WER-mediated patterning modulates planar polarity
JF  - Development : Company of Biologists
N2  - The coordination of cell polarity within the plane of the tissue layer (planar polarity) is crucial for the development of diverse multicellular organisms. Small Rac/Rho-family GTPases and the actin cytoskeleton contribute to planar polarity formation at sites of polarity establishment in animals and plants. Yet, upstream pathways coordinating planar polarity differ strikingly between kingdoms. In the root of Arabidopsis thaliana, a concentration gradient of the phytohormone auxin coordinates polar recruitment of Rho-of-plant (ROP) to sites of polar epidermal hair initiation. However, little is known about cytoskeletal components and interactions that contribute to this planar polarity or about their relation to the patterning machinery. Here, we show that ACTIN7 (ACT7) represents a main actin isoform required for planar polarity of root hair positioning, interacting with the negative modulator ACTIN-INTERACTING PROTEIN1-2 (AIP1-2). ACT7, AIP1-2 and their genetic interaction are required for coordinated planar polarity of ROP downstream of ethylene signalling. Strikingly, AIP1-2 displays hair cell file-enriched expression, restricted by WEREWOLF (WER)-dependent patterning and modified by ethylene and auxin action. Hence, our findings reveal AIP1-2, expressed under control of the WER-dependent patterning machinery and the ethylene signalling pathway, as a modulator of actin-mediated planar polarity.
KW  - AIP1
KW  - Arabidopsis
KW  - WEREWOLF
KW  - Actin
KW  - Patterning
KW  - Planar polarity
Y1  - 2015
U6  - https://doi.org/10.1242/dev.111013
SN  - 0950-1991
SN  - 1477-9129
VL  - 142
IS  - 1
SP  - 151
EP  - 161
PB  - Company of Biologists Limited
CY  - Cambridge
ER  -