TY  - JOUR
A1  - Morgner, Frank
A1  - Lecointre, Alexandre
A1  - Charbonniere, Loic J.
A1  - Löhmannsröben, Hans-Gerd
T1  - Detecting free hemoglobin in blood plasma and serum with luminescent terbium complexes
JF  - Physical chemistry, chemical physics : a journal of European Chemical Societies
N2  - Hemolysis, the rupturing of red blood cells, can result from numerous medical conditions (in vivo) or occur after collecting blood specimen or extracting plasma and serum out of whole blood (in vitro). In clinical laboratory practice, hemolysis can be a serious problem due to its potential to bias detection of various analytes or biomarkers. Here we present the first "mix-and-measure' method to assess the degree of hemolysis in biosamples using luminescence spectroscopy. Luminescent terbium complexes (LTC) were studied in the presence of free hemoglobin (Hb) as indicators for hemolysis in TRIS-buffer, and in fresh human plasma with absorption, excitation and emission measurements. Our findings indicate dynamic as well as resonance energy transfer (FRET) between the LTC and the porphyrin ligand of hemoglobin. This transfer leads to a decrease in luminescence intensity and decay time even at nanomolar hemoglobin concentrations either in buffer or plasma. Luminescent terbium complexes are very sensitive to free hemoglobin in buffer and blood plasma. Due to the instant change in luminescence properties of the LTC in presence of Hb it is possible to access the concentration of hemoglobin via spectroscopic methods without incubation time or further treatment of the sample thus enabling a rapid and sensitive detection of hemolysis in clinical diagnostics.
Y1  - 2015
U6  - https://doi.org/10.1039/c4cp04206a
SN  - 1463-9076
SN  - 1463-9084
VL  - 17
IS  - 3
SP  - 1740
EP  - 1745
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Morgner, Frank
A1  - Bennemann, Mark
A1  - Cywiński, Piotr J.
A1  - Kollosche, Matthias
A1  - Górski, Krzysztof
A1  - Pietraszkiewicz, Marek
A1  - Geßner, André
A1  - Löhmannsröben, Hans-Gerd
T1  - Elastic FRET sensors for contactless pressure measurement
JF  - RSC Advances : an international journal to further the chemical sciences
N2  - Contactless pressure monitoring based on Forster resonance energy transfer between donor/acceptor pairs immobilized within elastomers is demonstrated. The donor/acceptor energy transfer is employed by dispersing terbium(III) tris[(2-hydroxybenzoyl)-2-aminoethyl] amine complex (LLC, donor) and CdSe/ZnS quantum dots (QD655, acceptor) in styrene-ethylene/buthylene-styrene (SEBS) and poly(dimethylsiloxane) (PDMS). The continuous monitoring of QD luminescence showed a reversible intensity change as the pressure signal is alternated between two stable states indicating a pressure sensitivity of 6350 cps kPa(-1). Time-resolved measurements show the pressure impact on the FRET signal due to an increase of decay time (270 ms up to 420 ms) for the donor signal and parallel drop of decay time (170 mu s to 155 mu s) for the acceptor signal as the net pressure applied. The LLC/QD655 sensors enable a contactless readout as well as space resolved monitoring to enable miniaturization towards smaller integrated stretchable opto-electronics. Elastic FRET sensors can potentially lead to developing profitable analysis systems capable to outdo conventional wired electronic systems (inductive, capacitive, ultrasonic and photoelectric sensors) especially for point-of-care diagnostics, biological monitoring required for wearable electronics.
Y1  - 2017
U6  - https://doi.org/10.1039/c7ra06379b
SN  - 2046-2069
VL  - 7
SP  - 50578
EP  - 50583
PB  - RSC Publishing
CY  - Cambridge
ER  -