TY  - JOUR
A1  - Cywinski, Piotr J.
A1  - Idzik, Krzysztof R.
A1  - Cranfield, Charles G.
A1  - Beckert, Rainer
A1  - Mohr, Gerhard J.
T1  - Synthesis and sensing properties of a new carbazole fluorosensor for detection of abacavir
N2  - An abacavir-targeted fluorosensor based on the carbazole moiety has been synthesised and characterised. Recognition of abacavir is by base pairing between a uracil moiety present in the fluorosensor and the guanine moiety of abacavir. The fluorosensor exhibits five-fold quenching in the presence of 50M abacavir. Its sensitivity to abacavir is superior to that of other reverse transcriptase inhibitors: zidovudine, lamivudine and didanosine. Due to its high sensitivity, this fluorosensor has the potential to be used in multi-analyte array-based detection platforms as well as in microfluidics systems.
Y1  - 2010
UR  - http://www.informaworld.com/openurl?genre=journal&issn=1061-0278
U6  - https://doi.org/10.1080/10610278.2010.506541
SN  - 1061-0278
ER  - 
TY  - GEN
A1  - Idzik, Krzysztof Ryszard
A1  - Cywinski, Piotr J.
A1  - Cranfield, Charles G.
A1  - Mohr, Gerhard J.
A1  - Beckert, Rainer
T1  - Molecular recognition of the antiretroviral drug Abacavir
BT  - towards the development of a novel carbazole-based fluorosensor
T2  - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe
N2  - Due to their optical and electro-conductive attributes, carbazole derivatives are interesting materials for a large range of biosensor applications. In this study, we present the synthesis routes and fluorescence evaluation of newly designed carbazole fluorosensors that, by modification with uracil, have a special affinity for antiretroviral drugs via either Watson–Crick or Hoogsteen base pairing. To an N-octylcarbazole-uracil compound, four different groups were attached, namely thiophene, furane, ethylenedioxythiophene, and another uracil; yielding four different derivatives. Photophysical properties of these newly obtained derivatives are described, as are their interactions with the reverse transcriptase inhibitors such as abacavir, zidovudine, lamivudine and didanosine. The influence of each analyte on biosensor fluorescence was assessed on the basis of the Stern–Volmer equation and represented by Stern–Volmer constants. Consequently we have demonstrated that these structures based on carbazole, with a uracil group, may be successfully incorporated into alternative carbazole derivatives to form biosensors for the molecular recognition of antiretroviral drugs.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 847 
KW  - HIV
KW  - HAART
KW  - antiretroviral drugs
KW  - carbazole
KW  - base pairing
KW  - fluorescence spectroscopy
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-430372
SN  - 1866-8372
IS  - 847
SP  - 1195
EP  - 1204
ER  - 
TY  - JOUR
A1  - Idzik, Krzysztof Ryszard
A1  - Cywinski, Piotr J.
A1  - Cranfield, Charles G.
A1  - Mohr, Gerhard J.
A1  - Beckert, Rainer
T1  - Molecular recognition of the antiretroviral drug abacavir towards the development of a novel carbazole-based fluorosensor
JF  - Journal of fluorescence
N2  - Due to their optical and electro-conductive attributes, carbazole derivatives are interesting materials for a large range of biosensor applications. In this study, we present the synthesis routes and fluorescence evaluation of newly designed carbazole fluorosensors that, by modification with uracil, have a special affinity for antiretroviral drugs via either Watson-Crick or Hoogsteen base pairing. To an N-octylcarbazole-uracil compound, four different groups were attached, namely thiophene, furane, ethylenedioxythiophene, and another uracil; yielding four different derivatives. Photophysical properties of these newly obtained derivatives are described, as are their interactions with the reverse transcriptase inhibitors such as abacavir, zidovudine, lamivudine and didanosine. The influence of each analyte on biosensor fluorescence was assessed on the basis of the Stern-Volmer equation and represented by Stern-Volmer constants. Consequently we have demonstrated that these structures based on carbazole, with a uracil group, may be successfully incorporated into alternative carbazole derivatives to form biosensors for the molecular recognition of antiretroviral drugs.
KW  - HIV
KW  - HAART
KW  - Antiretroviral drugs
KW  - Carbazole
KW  - Base pairing
KW  - Fluorescence spectroscopy
Y1  - 2011
U6  - https://doi.org/10.1007/s10895-010-0798-7
SN  - 1053-0509
SN  - 1573-4994
VL  - 21
IS  - 3
SP  - 1195
EP  - 1204
PB  - Springer
CY  - New York
ER  -