TY  - GEN
A1  - Spricigo, Roberto
A1  - Dronov, Roman
A1  - Lisdat, Fred
A1  - Leimkühler, Silke
A1  - Scheller, Frieder W.
A1  - Wollenberger, Ursula
T1  - Electrocatalytic sulfite biosensor with human sulfite oxidase co-immobilized with cytochrome c in a polyelectrolyte-containing multilayer
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - An efficient electrocatalytic biosensor for sulfite detection was developed by co-immobilizing sulfite oxidase and cytochrome c with polyaniline sulfonic acid in a layer-by-layer assembly. QCM, UV-Vis spectroscopy and cyclic voltammetry revealed increasing loading of electrochemically active protein with the formation of multilayers. The sensor operates reagentless at low working potential. A catalytic oxidation current was detected in the presence of sulfite at the modified gold electrode, polarized at +0.1 V ( vs. Ag/AgCl 1 M KCl). The stability of the biosensor performance was characterized and optimized. A 17-bilayer electrode has a linear range between 1 and 60 mu M sulfite with a sensitivity of 2.19 mA M-1 sulfite and a response time of 2 min. The electrode retained a stable response for 3 days with a serial reproducibility of 3.8% and lost 20% of sensitivity after 5 days of operation. It is possible to store the sensor in a dry state for more than 2 months. The multilayer electrode was used for determination of sulfite in unspiked and spiked samples of red and white wine. The recovery and the specificity of the signals were evaluated for each sample.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 945 
KW  - bioelectrocatalysis
KW  - sulfite
KW  - sulfite oxidase
KW  - cytochrome c
KW  - multilayer
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-431176
SN  - 1866-8372
IS  - 945
SP  - 225
EP  - 233
ER  - 
TY  - JOUR
A1  - Krylov, Andrey. V.
A1  - Adamzig, H.
A1  - Walter, A. D.
A1  - Loechel, B.
A1  - Kurth, E.
A1  - Pulz, O.
A1  - Szeponik, Jan
A1  - Wegerich, Franziska
A1  - Lisdat, Fred
T1  - Parallel generation and detection of superoxide and hydrogen peroxide in a fluidic chip
JF  - Sensors and actuators : B, Chemical
N2  - A fluidic chip system was developed, which combines a stable generation of superoxide radicals and hydrogen peroxide with their sensorial detection. The generation of both reactive oxygen species was achieved by immobilization of xanthine oxidase on controlled pore glass in a reaction chamber. Antioxidants can be introduced into the fluidic chip system by means of mixing chamber. The detection of both species is based on the amperometric principle using a biosensor chip with two working electrodes. As sensing protein for both electrodes cytochrome c was used. The novel system was designed for the quantification of the antioxidant efficiency of different potential scavengers of the respective reactive species in an aqueous medium. Several model antioxidants such as ascorbic acid or catalase have been tested under flow conditions.
KW  - biosensor
KW  - cytochrome c
KW  - flow system
KW  - reactive oxygen species
KW  - antioxidant
Y1  - 2006
U6  - https://doi.org/10.1016/j.snb.2005.11.062
SN  - 0925-4005
VL  - 119
IS  - 1
SP  - 118
EP  - 126
PB  - Elsevier
CY  - Lausanne
ER  -