TY  - JOUR
A1  - Licausi, Francesco
A1  - Giorgi, Federico Manuel
A1  - Schmaelzlin, Elmar
A1  - Usadel, Björn
A1  - Perata, Pierdomenico
A1  - van Dongen, Joost Thomas
A1  - Geigenberger, Peter
T1  - HRE-Type Genes are regulated by Growth-Related Changes in internal Oxygen Concentrations During the normal development of Potato (Solanum tuberosum) Tubers
JF  - Plant & cell physiology
N2  - The occurrence of hypoxic conditions in plants not only represents a stress condition but is also associated with the normal development and growth of many organs, leading to adaptive changes in metabolism and growth to prevent internal anoxia. Internal oxygen concentrations decrease inside growing potato tubers, due to their active metabolism and increased resistance to gas diffusion as tubers grow. In the present work, we identified three hypoxia-responsive ERF (StHRE) genes whose expression is regulated by the gradual decrease in oxygen tensions that occur when potato tubers grow larger. Increasing the external oxygen concentration counteracted the modification of StHRE expression during tuber growth, supporting the idea that the actual oxygen levels inside the organs, rather than development itself, are responsible for the regulation of StHRE genes. We identified several sugar metabolism-related genes co-regulated with StHRE genes during tuber development and possibly involved in starch accumulation. All together, our data suggest a possible role for low oxygen in the regulation of sugar metabolism in the potato tuber, similar to what happens in storage tissues during seed development.
KW  - Co-expression
KW  - ERF
KW  - Hypoxia
KW  - Potato
KW  - Solanum tuberosum
KW  - Tuber
Y1  - 2011
U6  - https://doi.org/10.1093/pcp/pcr128
SN  - 0032-0781
VL  - 52
IS  - 11
SP  - 1957
EP  - 1972
PB  - Oxford Univ. Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Paparelli, Eleonora
A1  - Gonzali, Silvia
A1  - Parlanti, Sandro
A1  - Novi, Giacomo
A1  - Giorgi, Federico M.
A1  - Licausi, Francesco
A1  - Kosmacz, Monika
A1  - Feil, Regina
A1  - Lunn, John Edward
A1  - Brust, Henrike
A1  - van Dongen, Joost T.
A1  - Steup, Martin
A1  - Perata, Pierdomenico
T1  - Misexpression of a chloroplast aspartyl protease leads to severe growth defects and alters carbohydrate metabolism in arabidopsis
JF  - Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants
N2  - The crucial role of carbohydrate in plant growth and morphogenesis is widely recognized. In this study, we describe the characterization of nana, a dwarf Arabidopsis (Arabidopsis thaliana) mutant impaired in carbohydrate metabolism. We show that the nana dwarf phenotype was accompanied by altered leaf morphology and a delayed flowering time. Our genetic and molecular data indicate that the mutation in nana is due to a transfer DNA insertion in the promoter region of a gene encoding a chloroplast-located aspartyl protease that alters its pattern of expression. Overexpression of the gene (oxNANA) phenocopies the mutation. Both nana and oxNANA display alterations in carbohydrate content, and the extent of these changes varies depending on growth light intensity. In particular, in low light, soluble sugar levels are lower and do not show the daily fluctuations observed in wild-type plants. Moreover, nana and oxNANA are defective in the expression of some genes implicated in sugar metabolism and photosynthetic light harvesting. Interestingly, some chloroplast-encoded genes as well as genes whose products seem to be involved in retrograde signaling appear to be down-regulated. These findings suggest that the NANA aspartic protease has an important regulatory function in chloroplasts that not only influences photosynthetic carbon metabolism but also plastid and nuclear gene expression.
Y1  - 2012
U6  - https://doi.org/10.1104/pp.112.204016
SN  - 0032-0889
VL  - 160
IS  - 3
SP  - 1237
EP  - 1250
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  -