TY  - JOUR
A1  - Liang, Wei
A1  - Heinrich, Ingo
A1  - Simard, Sonia
A1  - Helle, Gerhard
A1  - Linan, Isabel Dorado
A1  - Heinken, Thilo
T1  - Climate signals derived from cell anatomy of Scots pine in NE Germany
JF  - Tree physiology
N2  - Tree-ring chronologies of Pinus sylvestris L. from latitudinal and altitudinal limits of the species distribution have been widely used for climate reconstructions, but there are many sites within the temperate climate zone, as is the case in northeastern Germany, at which there is little evidence of a clear climate signal in the chronologies. In this study, we developed long chronologies of several cell structure variables (e. g., average lumen area and cell wall thickness) from P. sylvestris growing in northeastern Germany and investigated the influence of climate on ring widths and cell structure variables. We found significant correlations between cell structure variables and temperature, and between tree-ring width and relative humidity and vapor pressure, respectively, enabling the development of robust reconstructions from temperate sites that have not yet been realized. Moreover, it has been shown that it may not be necessary to detrend chronologies of cell structure variables and thus low-frequency climate signals may be retrieved from longer cell structure chronologies. The relatively extensive resource of archaeological material of P. sylvestris covering approximately the last millennium may now be useful for climate reconstructions in northeastern Germany and other sites in the temperate climate zone.
KW  - cell structure
KW  - dendroclimatology
KW  - Pinus sylvestris
KW  - quantitative wood anatomy
KW  - tree rings
Y1  - 2013
U6  - https://doi.org/10.1093/treephys/tpt059
SN  - 0829-318X
SN  - 1758-4469
VL  - 33
IS  - 8
SP  - 833
EP  - 844
PB  - Oxford Univ. Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Liang, Wei
A1  - Heinrich, Ingo
A1  - Helle, Gerhard
A1  - Linan, Isabel Dorado
A1  - Heinken, Thilo
T1  - Applying CLSM to increment core surfaces for histometric analyses a novel advance in quantitative wood anatomy
JF  - Dendrochronologia : an interdisciplinary journal of tree-ring science
N2  - A novel procedure has been developed to conduct cell structure measurements on increment core samples of conifers. The procedure combines readily available hardware and software equipment. The essential part of the procedure is the application of a confocal laser scanning microscope (CLSM) which captures images directly from increment cores surfaced with the advanced WSL core-microtome. Cell wall and lumen are displayed with a strong contrast due to the monochrome black and green nature of the images. Consecutive images are merged into long images representing entire increment cores which are then analysed for cell structures in suitable software.
KW  - Wood anatomy
KW  - Cell structures
KW  - Confocal laser scanning microscopy
KW  - CLSM
KW  - Dendrochronology
KW  - Surface preparation
Y1  - 2013
U6  - https://doi.org/10.1016/j.dendro.2012.09.002
SN  - 1125-7865
VL  - 31
IS  - 2
SP  - 140
EP  - 145
PB  - Elsevier
CY  - Jena
ER  -