TY  - JOUR
A1  - Andresen, Heiko
A1  - Grötzinger, Carsten
A1  - Zarse, Kim
A1  - Birringer, Marc
A1  - Hessenius, Carsten
A1  - Kreuzer, Oliver Johannes
A1  - Ehrentreich-Förster, Eva
A1  - Bier, Frank Fabian
T1  - Peptide microarrays with site-specifically immobilized synthetic peptides for antibody diagnostics
N2  - Peptide microarrays bear the potential to discover molecular recognition events on protein level, particularly in the field of molecular immunology, in a manner and with an efficiency comparable to the performance of DNA microarrays. We developed a novel peptide microarray platform for the detection of antibodies in liquid samples. The system comprises site-specific solution phase coupling of biotinylated peptides to NeutrAvidin, localized microdispensing of peptide-NeutrAvidin conjugates onto activated glass slides and a fluorescence immuno sandwich assay format for antibody capture and detection. Our work includes synthetic peptides deduced from amino acid sequences of immunodominant linear epitopes, such as the T7 phage capsid protein, Herpes simplex virus glycoprotein D, c-myc protein and three domains of the Human coronavirus 229E polymerase polyprotein. We demonstrate that our method produces peptide arrays with excellent spot morphology which are capable of specific and sensitive detection of monoclonal antibodies from fluid samples.
Y1  - 2006
UR  - http://www.sciencedirect.com/science/journal/09254005
U6  - https://doi.org/10.1016/j.snb.2005.07.033
SN  - 0925-4005
ER  -