TY  - JOUR
A1  - Krüger, Anne
A1  - Batsios, Petros
A1  - Baumann, Otto
A1  - Luckert, Eva
A1  - Schwarz, Heinz
A1  - Stick, Reimer
A1  - Meyer, Irene
A1  - Gräf, Ralph
T1  - Characterization of NE81, the first lamin-like nucleoskeleton protein in a unicellular organism
JF  - Molecular biology of the cell : the official publication of the American Society for Cell Biology
N2  - Lamins build the nuclear lamina and are required for chromatin organization, gene expression, cell cycle progression, and mechanical stabilization. Despite these universal functions, lamins have so far been found only in metazoans. We have identified protein NE81 in Dictyostelium, which has properties that justify its denomination as a lamin-like protein in a lower eukaryote. This is based on its primary structure, subcellular localization, and regulation during mitosis, and its requirement of the C-terminal CaaX box as a posttranslational processing signal for proper localization. Our knockout and overexpression mutants revealed an important role for NE81 in nuclear integrity, chromatin organization, and mechanical stability of cells. All our results are in agreement with a role for NE81 in formation of a nuclear lamina. This function is corroborated by localization of Dictyostelium NE81 at the nuclear envelope in human cells. The discovery of a lamin-like protein in a unicellular organism is not only intriguing in light of evolution, it may also provide a simple experimental platform for studies of the molecular basis of laminopathies.
Y1  - 2012
U6  - https://doi.org/10.1091/mbc.E11-07-0595
SN  - 1059-1524
VL  - 23
IS  - 2
SP  - 360
EP  - 370
PB  - American Society for Cell Biology
CY  - Bethesda
ER  - 
TY  - JOUR
A1  - Neffe, Axel T.
A1  - von Rüsten-Lange, Maik
A1  - Braune, Steffen
A1  - Lützow, Karola
A1  - Roch, Toralf
A1  - Richau, Klaus
A1  - Krüger, Anne
A1  - Becherer, Tobias
A1  - Thünemann, Andreas F.
A1  - Jung, Friedrich
A1  - Haag, Rainer
A1  - Lendlein, Andreas
T1  - Multivalent grafting of hyperbranched oligo- and polyglycerols shielding rough membranes to mediate hemocompatibility
JF  - Journal of materials chemistry : B, Materials for biology and medicine
N2  - Hemocompatible materials are needed for internal and extracorporeal biomedical applications, which should be realizable by reducing protein and thrombocyte adhesion to such materials. Polyethers have been demonstrated to be highly efficient in this respect on smooth surfaces. Here, we investigate the grafting of oligo- and polyglycerols to rough poly(ether imide) membranes as a polymer relevant to biomedical applications and show the reduction of protein and thrombocyte adhesion as well as thrombocyte activation. It could be demonstrated that, by performing surface grafting with oligo-and polyglycerols of relatively high polydispersity (>1.5) and several reactive groups for surface anchoring, full surface shielding can be reached, which leads to reduced protein adsorption of albumin and fibrinogen. In addition, adherent thrombocytes were not activated. This could be clearly shown by immunostaining adherent proteins and analyzing the thrombocyte covered area. The presented work provides an important strategy for the development of application relevant hemocompatible 3D structured materials.
Y1  - 2014
U6  - https://doi.org/10.1039/c4tb00184b
SN  - 2050-750X
SN  - 2050-7518
VL  - 2
IS  - 23
SP  - 3626
EP  - 3635
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  -