TY  - JOUR
A1  - Sagu Tchewonpi, Sorel
A1  - Nso, Emmanuel Jong
A1  - Homann, Thomas
A1  - Kapseu, Cesar
A1  - Rawel, Harshadrai Manilal
T1  - Extraction and purification of beta-amylase from stems of Abrus precatorius by three phase partitioning
JF  - Food chemistry
N2  - The stems of Abrus precatorius were used to extract a beta-amylase enriched fraction. A three phase partitioning method and a Doehlert design with 3 variables (ratio of crude extract/t-butanol, the ammonium sulphate saturation and pH) were used. The data was fitted in a second-order polynomial model and the parameters were optimized to enrich beta-amylase. Experimental responses for the modulation were recovery of activity and the purification factor. The optimal conditions were: a ratio of crude extract/t-butanol of 0.87 (v/v), saturation in ammonium sulphate of 49.46% (w/v) and a pH of 5.2. An activity recovery of 156.2% and a purification factor of 10.17 were found. The enriched enzyme was identified as a beta-amylase and its molecular weight was 60.1 kDa. K-m and V-max values were 79.37 mg/ml and 5.13 U/ml, respectively and the highest activity was registered at a temperature of 70 degrees C and a pH between 6 and 6.5. A significant stabilization of the beta-amylase was observed up to 65 degrees C. (C) 2015 Elsevier Ltd. All rights reserved.
KW  - Purification
KW  - Beta-amylase
KW  - Abrus precatorius
KW  - Three phase partitioning
KW  - Doehlert design
Y1  - 2015
U6  - https://doi.org/10.1016/j.foodchem.2015.03.028
SN  - 0308-8146
SN  - 1873-7072
VL  - 183
SP  - 144
EP  - 153
PB  - Elsevier
CY  - Oxford
ER  -