TY  - JOUR
A1  - Bertz, Andreas
A1  - Wöhl-Bruhn, Stefanie
A1  - Miethe, Sebastian
A1  - Tiersch, Brigitte
A1  - Koetz, Joachim
A1  - Hust, Michael
A1  - Bunjes, Heike
A1  - Menzel, Henning
T1  - Encapsulation of proteins in hydrogel carrier systems for controlled drug delivery influence of network structure and drug size on release rate
JF  - Journal of biotechnology
N2  - Novel hydrogels based on hydroxyethyl starch modified with polyethylene glycol methacrylate (HES-P(EG)(6)MA) were developed as delivery system for the controlled release of proteins. Since the drug release behavior is supposed to be related to the pore structure of the hydrogel network the pore sizes were determined by cryo-SEM, which is a mild technique for imaging on a nanometer scale. The results showed a decreasing pore size and an increase in pore homogeneity with increasing polymer concentration. Furthermore, the mesh sizes of the hydrogels were calculated based on swelling data. Pore and mesh size were significantly different which indicates that both structures are present in the hydrogel. The resulting structural model was correlated with release data for bulk hydrogel cylinders loaded with FITC-dextran and hydrogel microspheres loaded with FITC-IgG and FITC-dextran of different molecular size. The initial release depended much on the relation between hydrodynamic diameter and pore size while the long term release of the incorporated substances was predominantly controlled by degradation of the network of the much smaller meshes.
KW  - Hydrogel
KW  - Hydrogel microspheres
KW  - Network structure
KW  - Release studies
KW  - Protein delivery
KW  - Mesh size
Y1  - 2013
U6  - https://doi.org/10.1016/j.jbiotec.2012.06.036
SN  - 0168-1656
VL  - 163
IS  - 2
SP  - 243
EP  - 249
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Connor, Daniel Oliver
A1  - Zantow, Jonas
A1  - Hust, Michael
A1  - Bier, Frank Fabian
A1  - von Nickisch-Rosenegk, Markus
T1  - Identification of Novel Immunogenic Proteins of Neisseria gonorrhoeae by Phage Display
JF  - PLoS one
N2  - Neisseria gonorrhoeae is one of the most prevalent sexually transmitted diseases worldwide with more than 100 million new infections per year. A lack of intense research over the last decades and increasing resistances to the recommended antibiotics call for a better understanding of gonococcal infection, fast diagnostics and therapeutic measures against N. gonorrhoeae. Therefore, the aim of this work was to identify novel immunogenic proteins as a first step to advance those unresolved problems. For the identification of immunogenic proteins, pHORF oligopeptide phage display libraries of the entire N. gonorrhoeae genome were constructed. Several immunogenic oligopeptides were identified using polyclonal rabbit antibodies against N. gonorrhoeae. Corresponding full-length proteins of the identified oligopeptides were expressed and their immunogenic character was verified by ELISA. The immunogenic character of six proteins was identified for the first time. Additional 13 proteins were verified as immunogenic proteins in N. gonorrhoeae.
Y1  - 2016
U6  - https://doi.org/10.1371/journal.pone.0148986
SN  - 1932-6203
VL  - 11
PB  - PLoS
CY  - San Fransisco
ER  -