TY  - JOUR
A1  - Hildebrandt, Niko
A1  - Geissler, Daniel
ED  - Zahavy, E
ED  - Ordentlich, A
ED  - Yitzhaki, S
ED  - Shafferman, A
T1  - Semiconductor quantum dots as FRET acceptors for multiplexed diagnostics and molecular ruler application
JF  - Advances in Experimental Medicine and Biology
N2  - Applications based on Forster resonance energy transfer (FRET) play an important role for the determination of concentrations and distances within nanometer-scale systems in vitro and in vivo in many fields of biotechnology. Semiconductor nanocrystals (Quantum dots - QDs) possess ideal properties for their application as FRET acceptors when the donors have long excited state lifetimes and when direct excitation of QDs can be efficiently suppressed. Therefore, luminescent terbium complexes (LTCs) with excited state lifetimes of more than 2 ms are ideal FRET donor candidates for QD-acceptors. This chapter will give a short overview of theoretical and practical background of FRET, QDs and LTCs, and present some recent applications of LTC-QD FRET pairs for multiplexed ultra-sensitive in vitro diagnostics and nanometer-resolution molecular distance measurements.
KW  - Diagnostics
KW  - FRET
KW  - Imaging
KW  - Quantum dots
KW  - Terbium
Y1  - 2012
SN  - 978-94-007-2554-6
SN  - 978-94-007-2555-3
U6  - https://doi.org/10.1007/978-94-007-2555-3_8
SN  - 0065-2598
VL  - 733
SP  - 75
EP  - 86
PB  - Springer
CY  - Dordrecht
ER  -