TY  - JOUR
A1  - Henze, Andrea
A1  - Frey, Simone K.
A1  - Raila, Jens
A1  - Scholze, Alexandra
A1  - Spranger, Joachim
A1  - Weickert, Martin O.
A1  - Tepel, Martin
A1  - Zidek, Walter
A1  - Schweigert, Florian J.
T1  - Alterations of retinol-binding protein 4 species in patients with different stages of chronic kidney disease and their relation to lipid parameters
N2  - Retinol-binding protein 4 (RBP4) is elevated in patients with chronic kidney disease (CKD) and has been discussed as marker of kidney function. In addition to an elevated concentration, the existence of truncated RBP4 species, RBP4-L (truncated at last C-terminal leucine) and RBP4-LL (truncated at both C-terminal leucines), has been reported in serum of hemodialysis patients. Since little is known about the occurrence of RBP4 species during the progression of CKD it was the aim of this study to analyse this possible association. The presence of RBP4, RBP4-L, RBP4- LL and transthyretin (TTR) was assessed in serum of 45 healthy controls and 52 patients with stage 2-5 of CKD using ELISA and RBP4 immunoprecipitation with subsequent MALDI-TOF-MS analysis. A reduction of glomerular filtration rate was accompanied by a gradual elevation of RBP4 serum levels and relative amounts of RBP4-LL. Correlation analysis revealed a strong association of the RBP4-TTR ratio with parameters of lipid metabolism and with diabetes-related factors. In conclusion, RBP4 serum concentration and the appearance of RBP4-LL seem to be influenced by kidney function. Furthermore, the RBP4-TTR ratio may provide diagnostic potential with regard to metabolic complications in CKD patients.
Y1  - 2010
UR  - http://www.sciencedirect.com/science/journal/0006291X
U6  - https://doi.org/10.1016/j.bbrc.2010.01.082
SN  - 0006-291X
ER  - 
TY  - JOUR
A1  - Bobbert, Thomas
A1  - Raila, Jens
A1  - Schwarz, Franziska
A1  - Mai, Knut
A1  - Henze, Andrea
A1  - Pfeiffer, Andreas F. H.
A1  - Schweigert, Florian J.
A1  - Spranger, Joachim
T1  - Relation between retinol, retinol-binding protein 4, transthyretin and carotid intima media thickness
N2  - Objective: Retinol is transported in a complex with retinol-binding protein 4 (RBP4) and transthyretin (TTR) in the circulation. While retinol is associated with various cardiovascular risk factors, the relation between retinol, RBP4, TTR and carotid intima media thickness (IMT) has not been analysed yet. Methods: Retinol, RBP4 and TTR were measured in 96 individuals and their relation to mean and maximal IMT was determined. Results: Mean IMT correlated with RBP4 (r = 0.335, p < 0.001), retinol (r = -0.241, p = 0.043), RBP/TTR ratio (r = 0.254, p = 0.025) and retinol/RBP4 ratio (r = -0.549, p < 0.001). Adjustment for age, sex, BMI, blood pressure, HDL/total cholesterol ratio, triglyceride, diabetes and smoking revealed that the retinol/RBP4 ratio was strongly and independently associated with mean IMT. Similar results were found for maximal IMT, which included the measurement of plaques. Conclusion: The data support that the transport complex of vitamin A is associated with the IMT, an established parameter of atherosclerosis. Changes in RBP4 saturation with retinol may link renal dysfunction and insulin resistance to atherosclerosis.
Y1  - 2010
UR  - http://www.sciencedirect.com/science/journal/00219150
U6  - https://doi.org/10.1016/j.atherosclerosis.2010.07.063
SN  - 0021-9150
ER  -