TY  - JOUR
A1  - Messerschmidt, Katrin
A1  - Heilmann, Katja
T1  - Toxin-antigen conjugates as selection tools for antibody producing cells
JF  - Journal of immunological methods
N2  - The generation of antibodies with designated specificity requires cost-intensive and time-consuming screening procedures. Here we present a new method by which hybridoma cells can be selected based on the specificity of the produced antibody by the use of antigen-toxin-conjugates thus eliminating the need of a screening procedure. Initial experiments were done with methotrexate as low molecular weight toxin and fluorescein as model antigen. Methotrexate and a methotrexate-fluorescein conjugate were characterized regarding their toxicity. Afterwards the effect of the fluorescein-specific antibody B13-DE1 on the toxicity of the methotrexate-fluorescein conjugate was determined. Finally, first results showed that hybridoma cells that produce fluorescein specific antibodies are able to grow in the presence of fluorescein-toxin-conjugates.
KW  - Monoclonal antibody
KW  - Hybridoma technology
KW  - Selection of antibody producing cells
Y1  - 2013
U6  - https://doi.org/10.1016/j.jim.2012.10.010
SN  - 0022-1759
VL  - 387
IS  - 1-2
SP  - 167
EP  - 172
PB  - Elsevier
CY  - Amsterdam
ER  -