TY  - GEN
A1  - Göritz, Anna
A1  - Berger, Stella A.
A1  - Gege, Peter
A1  - Grossart, Hans-Peter
A1  - Nejstgaard, Jens C.
A1  - Riedel, Sebastian
A1  - Röttgers, Rüdiger
A1  - Utschig, Christian
T1  - Retrieval of water constituents from hyperspectral in-situ measurements under variable cloud cover
BT  - a case study at lake Stechlin (Germany)
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Remote sensing and field spectroscopy of natural waters is typically performed under clear skies, low wind speeds and low solar zenith angles. Such measurements can also be made, in principle, under clouds and mixed skies using airborne or in-situ measurements; however, variable illumination conditions pose a challenge to data analysis. In the present case study, we evaluated the inversion of hyperspectral in-situ measurements for water constituent retrieval acquired under variable cloud cover. First, we studied the retrieval of Chlorophyll-a (Chl-a) concentration and colored dissolved organic matter (CDOM) absorption from in-water irradiance measurements. Then, we evaluated the errors in the retrievals of the concentration of total suspended matter (TSM), Chl-a and the absorption coefficient of CDOM from above-water reflectance measurements due to highly variable reflections at the water surface. In order to approximate cloud reflections, we extended a recent three-component surface reflectance model for cloudless atmospheres by a constant offset and compared different surface reflectance correction procedures. Our findings suggest that in-water irradiance measurements may be used for the analysis of absorbing compounds even under highly variable weather conditions. The extended surface reflectance model proved to contribute to the analysis of above-water reflectance measurements with respect to Chl-a and TSM. Results indicate the potential of this approach for all-weather monitoring.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 941 
KW  - remote sensing
KW  - inland water
KW  - hyperspectral measurements
KW  - in-situ
KW  - cloud
KW  - surface reflection
KW  - inversion
KW  - bio-optical modeling
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-459837
SN  - 1866-8372
IS  - 941
ER  - 
TY  - GEN
A1  - Garcia, Sarahi L.
A1  - Buck, Moritz
A1  - Hamilton, Joshua J.
A1  - Wurzbacher, Christian
A1  - Grossart, Hans-Peter
A1  - McMahon, Katherine D.
A1  - Eiler, Alexander
T1  - Model communities hint at promiscuous metabolic linkages between ubiquitous free-living freshwater bacteria
T2  - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe
N2  - Genome streamlining is frequently observed in free-living aquatic microorganisms and results in physiological dependencies between microorganisms. However, we know little about the specificity of these microbial associations. In order to examine the specificity and extent of these associations, we established mixed cultures from three different freshwater environments and analyzed the cooccurrence of organisms using a metagenomic time series. Free-living microorganisms with streamlined genomes lacking multiple biosynthetic pathways showed no clear recurring pattern in their interaction partners. Free-living freshwater bacteria form promiscuous cooperative associations. This notion contrasts with the well-documented high specificities of interaction partners in host-associated bacteria. Considering all data together, we suggest that highly abundant free-living bacterial lineages are functionally versatile in their interactions despite their distinct streamlining tendencies at the single-cell level. This metabolic versatility facilitates interactions with a variable set of community members.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 714 
KW  - community
KW  - interactions
KW  - metagenomics
KW  - microbial ecology
KW  - mixed cultures
KW  - promiscuous
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-427299
IS  - 714
ER  - 
TY  - GEN
A1  - Fabian, Jenny
A1  - Zlatanović, Sanja
A1  - Mutz, Michael
A1  - Grossart, Hans-Peter
A1  - Geldern, Robert van
A1  - Ulrich, Andreas
A1  - Gleixner, Gerd
A1  - Premke, Katrin
T1  - Environmental control on microbial turnover of leaf carbon in streams
BT  - ecological function of phototrophic-heterotrophic interactions
T2  - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe
N2  - In aquatic ecosystems, light availability can significantly influence microbial turnover of terrestrial organic matter through associated metabolic interactions between phototrophic and heterotrophic communities. However, particularly in streams, microbial functions vary significantly with the structure of the streambed, that is the distribution and spatial arrangement of sediment grains in the streambed. It is therefore essential to elucidate how environmental factors synergistically define the microbial turnover of terrestrial organic matter in order to better understand the ecological role of photo-heterotrophic interactions in stream ecosystem processes. In outdoor experimental streams, we examined how the structure of streambeds modifies the influence of light availability on microbial turnover of leaf carbon (C). Furthermore, we investigated whether the studied relationships of microbial leaf C turnover to environmental conditions are affected by flow intermittency commonly occurring in streams. We applied leaves enriched with a 13C-stable isotope tracer and combined quantitative and isotope analyses. We thereby elucidated whether treatment induced changes in C turnover were associated with altered use of leaf C within the microbial food web. Moreover, isotope analyses were combined with measurements of microbial community composition to determine whether changes in community function were associated with a change in community composition. In this study, we present evidence, that environmental factors interactively determine how phototrophs and heterotrophs contribute to leaf C turnover. Light availability promoted the utilization of leaf C within the microbial food web, which was likely associated with a promoted availability of highly bioavailable metabolites of phototrophic origin. However, our results additionally confirm that the structure of the streambed modifies light-related changes in microbial C turnover. From our observations, we conclude that the streambed structure influences the strength of photo-heterotrophic interactions by defining the spatial availability of algal metabolites in the streambed and the composition of microbial communities. Collectively, our multifactorial approach provides valuable insights into environmental controls on the functioning of stream ecosystems.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 693 
KW  - algae
KW  - bacteria
KW  - microbial interactions
KW  - 13C stable isotopes
KW  - PLFA
KW  - terrestrial carbon
KW  - streambed structure
KW  - light
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-426336
SN  - 1866-8372
IS  - 693
ER  - 
TY  - GEN
A1  - Cuadrat, Rafael R. C.
A1  - Ionescu, Danny
A1  - Dávila, Alberto M. R.
A1  - Grossart, Hans-Peter
T1  - Recovering genomics clusters of secondary metabolites from lakes using genome-resolved metagenomics
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Metagenomic approaches became increasingly popular in the past decades due to decreasing costs of DNA sequencing and bioinformatics development. So far, however, the recovery of long genes coding for secondary metabolites still represents a big challenge. Often, the quality of metagenome assemblies is poor, especially in environments with a high microbial diversity where sequence coverage is low and complexity of natural communities high. Recently, new and improved algorithms for binning environmental reads and contigs have been developed to overcome such limitations. Some of these algorithms use a similarity detection approach to classify the obtained reads into taxonomical units and to assemble draft genomes. This approach, however, is quite limited since it can classify exclusively sequences similar to those available (and well classified) in the databases. In this work, we used draft genomes from Lake Stechlin, north-eastern Germany, recovered by MetaBat, an efficient binning tool that integrates empirical probabilistic distances of genome abundance, and tetranucleotide frequency for accurate metagenome binning. These genomes were screened for secondary metabolism genes, such as polyketide synthases (PKS) and non-ribosomal peptide synthases (NRPS), using the Anti-SMASH and NAPDOS workflows. With this approach we were able to identify 243 secondary metabolite clusters from 121 genomes recovered from our lake samples. A total of 18 NRPS, 19 PKS, and 3 hybrid PKS/NRPS clusters were found. In addition, it was possible to predict the partial structure of several secondary metabolite clusters allowing for taxonomical classifications and phylogenetic inferences. Our approach revealed a high potential to recover and study secondary metabolites genes from any aquatic ecosystem.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 924 
KW  - metagenomics 2.0
KW  - PKS
KW  - NRPS
KW  - freshwater
KW  - environmental genomics
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-445656
SN  - 1866-8372
IS  - 924
ER  - 
TY  - GEN
A1  - Mantzouki, Evanthia
A1  - Lürling, Miquel
A1  - Fastner, Jutta
A1  - Domis, Lisette Nicole de Senerpont
A1  - Wilk-Woźniak, Elżbieta
A1  - Koreiviene, Judita
A1  - Seelen, Laura
A1  - Teurlincx, Sven
A1  - Verstijnen, Yvon
A1  - Krztoń, Wojciech
A1  - Walusiak, Edward
A1  - Karosienė, Jūratė
A1  - Kasperovičienė, Jūratė
A1  - Savadova, Ksenija
A1  - Vitonytė, Irma
A1  - Cillero-Castro, Carmen
A1  - Budzyńska, Agnieszka
A1  - Goldyn, Ryszard
A1  - Kozak, Anna
A1  - Rosińska, Joanna
A1  - Szeląg-Wasielewska, Elżbieta
A1  - Domek, Piotr
A1  - Jakubowska-Krepska, Natalia
A1  - Kwasizur, Kinga
A1  - Messyasz, Beata
A1  - Pełechata, Aleksandra
A1  - Pełechaty, Mariusz
A1  - Kokocinski, Mikolaj
A1  - García-Murcia, Ana
A1  - Real, Monserrat
A1  - Romans, Elvira
A1  - Noguero-Ribes, Jordi
A1  - Duque, David Parreño
A1  - Fernández-Morán, Elísabeth
A1  - Karakaya, Nusret
A1  - Häggqvist, Kerstin
A1  - Beklioğlu, Meryem
A1  - Filiz, Nur
A1  - Levi, Eti E.
A1  - Iskin, Uğur
A1  - Bezirci, Gizem
A1  - Tavşanoğlu, Ülkü Nihan
A1  - Özhan, Koray
A1  - Gkelis, Spyros
A1  - Panou, Manthos
A1  - Fakioglu, Özden
A1  - Avagianos, Christos
A1  - Kaloudis, Triantafyllos
A1  - Çelik, Kemal
A1  - Yilmaz, Mete
A1  - Marcé, Rafael
A1  - Catalán, Nuria
A1  - Bravo, Andrea G.
A1  - Buck, Moritz
A1  - Colom-Montero, William
A1  - Mustonen, Kristiina
A1  - Pierson, Don
A1  - Yang, Yang
A1  - Raposeiro, Pedro M.
A1  - Gonçalves, Vítor
A1  - Antoniou, Maria G.
A1  - Tsiarta, Nikoletta
A1  - McCarthy, Valerie
A1  - Perello, Victor C.
A1  - Feldmann, Tõnu
A1  - Laas, Alo
A1  - Panksep, Kristel
A1  - Tuvikene, Lea
A1  - Gagala, Ilona
A1  - Mankiewicz-Boczek, Joana
A1  - Yağcı, Meral Apaydın
A1  - Çınar, Şakir
A1  - Çapkın, Kadir
A1  - Yağcı, Abdulkadir
A1  - Cesur, Mehmet
A1  - Bilgin, Fuat
A1  - Bulut, Cafer
A1  - Uysal, Rahmi
A1  - Obertegger, Ulrike
A1  - Boscaini, Adriano
A1  - Flaim, Giovanna
A1  - Salmaso, Nico
A1  - Cerasino, Leonardo
A1  - Richardson, Jessica
A1  - Visser, Petra M.
A1  - Verspagen, Jolanda M. H.
A1  - Karan, Tünay
A1  - Soylu, Elif Neyran
A1  - Maraşlıoğlu, Faruk
A1  - Napiórkowska-Krzebietke, Agnieszka
A1  - Ochocka, Agnieszka
A1  - Pasztaleniec, Agnieszka
A1  - Antão-Geraldes, Ana M.
A1  - Vasconcelos, Vitor
A1  - Morais, João
A1  - Vale, Micaela
A1  - Köker, Latife
A1  - Akçaalan, Reyhan
A1  - Albay, Meriç
A1  - Maronić, Dubravka Špoljarić
A1  - Stević, Filip
A1  - Pfeiffer, Tanja Žuna
A1  - Fonvielle, Jeremy Andre
A1  - Straile, Dietmar
A1  - Rothhaupt, Karl-Otto
A1  - Hansson, Lars-Anders
A1  - Urrutia-Cordero, Pablo
A1  - Bláha, Luděk
A1  - Geriš, Rodan
A1  - Fránková, Markéta
A1  - Koçer, Mehmet Ali Turan
A1  - Alp, Mehmet Tahir
A1  - Remec-Rekar, Spela
A1  - Elersek, Tina
A1  - Triantis, Theodoros
A1  - Zervou, Sevasti-Kiriaki
A1  - Hiskia, Anastasia
A1  - Haande, Sigrid
A1  - Skjelbred, Birger
A1  - Madrecka, Beata
A1  - Nemova, Hana
A1  - Drastichova, Iveta
A1  - Chomova, Lucia
A1  - Edwards, Christine
A1  - Sevindik, Tuğba Ongun
A1  - Tunca, Hatice
A1  - Önem, Burçin
A1  - Aleksovski, Boris
A1  - Krstić, Svetislav
A1  - Vucelić, Itana Bokan
A1  - Nawrocka, Lidia
A1  - Salmi, Pauliina
A1  - Machado-Vieira, Danielle
A1  - Oliveira, Alinne Gurjão De
A1  - Delgado-Martín, Jordi
A1  - García, David
A1  - Cereijo, Jose Luís
A1  - Gomà, Joan
A1  - Trapote, Mari Carmen
A1  - Vegas-Vilarrúbia, Teresa
A1  - Obrador, Biel
A1  - Grabowska, Magdalena
A1  - Karpowicz, Maciej
A1  - Chmura, Damian
A1  - Úbeda, Bárbara
A1  - Gálvez, José Ángel
A1  - Özen, Arda
A1  - Christoffersen, Kirsten Seestern
A1  - Warming, Trine Perlt
A1  - Kobos, Justyna
A1  - Mazur-Marzec, Hanna
A1  - Pérez-Martínez, Carmen
A1  - Ramos-Rodríguez, Eloísa
A1  - Arvola, Lauri
A1  - Alcaraz-Párraga, Pablo
A1  - Toporowska, Magdalena
A1  - Pawlik-Skowronska, Barbara
A1  - Niedźwiecki, Michał
A1  - Pęczuła, Wojciech
A1  - Leira, Manel
A1  - Hernández, Armand
A1  - Moreno-Ostos, Enrique
A1  - Blanco, José María
A1  - Rodríguez, Valeriano
A1  - Montes-Pérez, Jorge Juan
A1  - Palomino, Roberto L.
A1  - Rodríguez-Pérez, Estela
A1  - Carballeira, Rafael
A1  - Camacho, Antonio
A1  - Picazo, Antonio
A1  - Rochera, Carlos
A1  - Santamans, Anna C.
A1  - Ferriol, Carmen
A1  - Romo, Susana
A1  - Soria, Juan Miguel
A1  - Dunalska, Julita
A1  - Sieńska, Justyna
A1  - Szymański, Daniel
A1  - Kruk, Marek
A1  - Kostrzewska-Szlakowska, Iwona
A1  - Jasser, Iwona
A1  - Žutinić, Petar
A1  - Udovič, Marija Gligora
A1  - Plenković-Moraj, Anđelka
A1  - Frąk, Magdalena
A1  - Bańkowska-Sobczak, Agnieszka
A1  - Wasilewicz, Michał
A1  - Özkan, Korhan
A1  - Maliaka, Valentini
A1  - Kangro, Kersti
A1  - Grossart, Hans-Peter
A1  - Paerl, Hans W.
A1  - Carey, Cayelan C.
A1  - Ibelings, Bas W.
T1  - Temperature effects explain continental scale distribution of cyanobacterial toxins
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Insight into how environmental change determines the production and distribution of cyanobacterial toxins is necessary for risk assessment. Management guidelines currently focus on hepatotoxins (microcystins). Increasing attention is given to other classes, such as neurotoxins (e.g., anatoxin-a) and cytotoxins (e.g., cylindrospermopsin) due to their potency. Most studies examine the relationship between individual toxin variants and environmental factors, such as nutrients, temperature and light. In summer 2015, we collected samples across Europe to investigate the effect of nutrient and temperature gradients on the variability of toxin production at a continental scale. Direct and indirect effects of temperature were the main drivers of the spatial distribution in the toxins produced by the cyanobacterial community, the toxin concentrations and toxin quota. Generalized linear models showed that a Toxin Diversity Index (TDI) increased with latitude, while it decreased with water stability. Increases in TDI were explained through a significant increase in toxin variants such as MC-YR, anatoxin and cylindrospermopsin, accompanied by a decreasing presence of MC-LR. While global warming continues, the direct and indirect effects of increased lake temperatures will drive changes in the distribution of cyanobacterial toxins in Europe, potentially promoting selection of a few highly toxic species or strains.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1105 
KW  - microcystin
KW  - anatoxin
KW  - cylindrospermopsin
KW  - temperature
KW  - direct effects
KW  - indirect effects
KW  - spatial distribution
KW  - European Multi Lake Survey
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-427902
SN  - 1866-8372
IS  - 1105
ER  - 
TY  - GEN
A1  - Bálint, Miklós
A1  - Márton, Orsolya
A1  - Schatz, Marlene
A1  - Düring, Rolf-Alexander
A1  - Grossart, Hans-Peter
T1  - Proper experimental design requires randomization/balancing of molecular ecology experiments
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Properly designed (randomized and/or balanced) experiments are standard in ecological research. Molecular methods are increasingly used in ecology, but studies generally do not report the detailed design of sample processing in the laboratory. This may strongly influence the interpretability of results if the laboratory procedures do not account for the confounding effects of unexpected laboratory events. We demonstrate this with a simple experiment where unexpected differences in laboratory processing of samples would have biased results if randomization in DNA extraction and PCR steps do not provide safeguards. We emphasize the need for proper experimental design and reporting of the laboratory phase of molecular ecology research to ensure the reliability and interpretability of results.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 616 
KW  - batch effect
KW  - bias
KW  - DNA extraction
KW  - environmental DNA
KW  - laboratory practice
KW  - lake community
KW  - metabarcoding
KW  - nondemonic intrusions
KW  - PCR
KW  - sediment
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-423878
SN  - 1866-8372
IS  - 616
ER  -