TY  - JOUR
A1  - Sachse, Rita
A1  - Wüstenhagen, Doreen Anja
A1  - Samalikova, Maria
A1  - Gerrits, Michael
A1  - Bier, Frank Fabian
A1  - Kubick, Stefan
T1  - Synthesis of membrane proteins in eukaryotic cell-free systems
JF  - Engineering in life sciences : Industry, Environment, Plant, Food
N2  - Cell-free protein synthesis (CFPS) is a valuable method for the fast expression of difficult-to-express proteins as well as posttranslationally modified proteins. Since cell-free systems circumvent possible cytotoxic effects caused by protein overexpression in living cells, they significantly enlarge the scale and variety of proteins that can be characterized. We demonstrate the high potential of eukaryotic CFPS to express various types of membrane proteins covering a broad range of structurally and functionally diverse proteins. Our eukaryotic cell-free translation systems are capable to provide high molecular weight membrane proteins, fluorescent-labeled membrane proteins, as well as posttranslationally modified proteins for further downstream analysis.
KW  - Cell-free protein expression
KW  - In vitro protein synthesis
KW  - Labeled membrane proteins
KW  - Synthetic glycoprotein
Y1  - 2013
U6  - https://doi.org/10.1002/elsc.201100235
SN  - 1618-0240
VL  - 13
IS  - 1
SP  - 39
EP  - 48
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -