TY  - JOUR
A1  - Baessler, Olivia Y.
A1  - Weiss, Julia
A1  - Wienkoop, Stefanie
A1  - Lehmann, Karola
A1  - Scheler, Christian
A1  - Doelle, Sabine
A1  - Schwarz, Dietmar
A1  - Franken, Philipp
A1  - George, Eckhard
A1  - Worm, Margitta
A1  - Weckwerth, Wolfram
T1  - Evidence for novel tomato seed allergens : IgE-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling
N2  - Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomato-sensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins.
Y1  - 2009
UR  - http://pubs.acs.org/journal/jprobs
U6  - https://doi.org/10.1021/Pr800186d
SN  - 1535-3893
ER  - 
TY  - JOUR
A1  - Cosme, Marco
A1  - Franken, Philipp
A1  - Mewis, Inga
A1  - Baldermann, Susanne
A1  - Wurst, Susanne
T1  - Arbuscular mycorrhizal fungi affect glucosinolate and mineral element composition in leaves of Moringa oleifera
JF  - Mycorrhiza
N2  - Moringa is a mycorrhizal crop cultivated in the tropics and subtropics and appreciated for its nutritive and health-promoting value. As well as improving plant mineral nutrition, arbuscular mycorrhizal fungi (AMF) can affect plant synthesis of compounds bioactive against chronic diseases in humans. Rhizophagus intraradices and Funneliformis mosseae were used in a full factorial experiment to investigate the impact of AMF on the accumulation of glucosinolates, flavonoids, phenolic acids, carotenoids, and mineral elements in moringa leaves. Levels of glucosinolates were enhanced, flavonoids and phenolic acids were not affected, levels of carotenoids (including provitamin A) were species-specifically reduced, and mineral elements were affected differently, with only Cu and Zn being increased by the AMF. This study presents novel results on AMF effects on glucosinolates in leaves and supports conclusions that the impacts of these fungi on microelement concentrations in edible plants are species dependent. The nonspecific positive effects on glucosinolates and the species-specific negative effects on carotenoids encourage research on other AMF species to achieve general benefits on bioactive compounds in moringa.
KW  - Arbuscularmycorrhizal fungi
KW  - Moringa oleifera
KW  - Crop quality
KW  - Health-promoting compounds
KW  - Microelements
Y1  - 2014
U6  - https://doi.org/10.1007/s00572-014-0574-7
SN  - 0940-6360
SN  - 1432-1890
VL  - 24
IS  - 7
SP  - 565
EP  - 570
PB  - Springer
CY  - New York
ER  -