TY  - JOUR
A1  - Näther, Astrid
A1  - Fösel, Bärbel U.
A1  - Nägele, Verena
A1  - Wüst, Pia K.
A1  - Weinert, Jan
A1  - Bonkowski, Michael
A1  - Alt, Fabian
A1  - Oelmann, Yvonne
A1  - Polle, Andrea
A1  - Lohaus, Gertrud
A1  - Gockel, Sonja
A1  - Hemp, Andreas
A1  - Kalko, Elisabeth K. V.
A1  - Linsenmair, Karl Eduard
A1  - Pfeiffer, Simone
A1  - Renner, Swen
A1  - Schöning, Ingo
A1  - Weisser, Wolfgang W.
A1  - Wells, Konstans
A1  - Fischer, Markus
A1  - Overmann, Jörg
A1  - Friedrich, Michael W.
T1  - Environmental factors affect acidobacterial communities below the subgroup level in Grassland and Forest Soils
JF  - Applied and environmental microbiology
N2  - In soil, Acidobacteria constitute on average 20% of all bacteria, are highly diverse, and are physiologically active in situ. However, their individual functions and interactions with higher taxa in soil are still unknown. Here, potential effects of land use, soil properties, plant diversity, and soil nanofauna on acidobacterial community composition were studied by cultivation-independent methods in grassland and forest soils from three different regions in Germany. The analysis of 16S rRNA gene clone libraries representing all studied soils revealed that grassland soils were dominated by subgroup Gp6 and forest soils by subgroup Gp1 Acidobacteria. The analysis of a large number of sites (n = 57) by 16S rRNA gene fingerprinting methods (terminal restriction fragment length polymorphism [T-RFLP] and denaturing gradient gel electrophoresis [DGGE]) showed that Acidobacteria diversities differed between grassland and forest soils but also among the three different regions. Edaphic properties, such as pH, organic carbon, total nitrogen, C/N ratio, phosphorus, nitrate, ammonium, soil moisture, soil temperature, and soil respiration, had an impact on community composition as assessed by fingerprinting. However, interrelations with environmental parameters among subgroup terminal restriction fragments (T-RFs) differed significantly, e.g., different Gp1 T-RFs correlated positively or negatively with nitrogen content. Novel significant correlations of Acidobacteria subpopulations (i.e., individual populations within subgroups) with soil nanofauna and vascular plant diversity were revealed only by analysis of clone sequences. Thus, for detecting novel interrelations of environmental parameters with Acidobacteria, individual populations within subgroups have to be considered.
Y1  - 2012
U6  - https://doi.org/10.1128/AEM.01325-12
SN  - 0099-2240
VL  - 78
IS  - 20
SP  - 7398
EP  - 7406
PB  - American Society for Microbiology
CY  - Washington
ER  -