TY  - GEN
A1  - Garcia, Sarahi L.
A1  - Buck, Moritz
A1  - Hamilton, Joshua J.
A1  - Wurzbacher, Christian
A1  - Grossart, Hans-Peter
A1  - McMahon, Katherine D.
A1  - Eiler, Alexander
T1  - Model communities hint at promiscuous metabolic linkages between ubiquitous free-living freshwater bacteria
T2  - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe
N2  - Genome streamlining is frequently observed in free-living aquatic microorganisms and results in physiological dependencies between microorganisms. However, we know little about the specificity of these microbial associations. In order to examine the specificity and extent of these associations, we established mixed cultures from three different freshwater environments and analyzed the cooccurrence of organisms using a metagenomic time series. Free-living microorganisms with streamlined genomes lacking multiple biosynthetic pathways showed no clear recurring pattern in their interaction partners. Free-living freshwater bacteria form promiscuous cooperative associations. This notion contrasts with the well-documented high specificities of interaction partners in host-associated bacteria. Considering all data together, we suggest that highly abundant free-living bacterial lineages are functionally versatile in their interactions despite their distinct streamlining tendencies at the single-cell level. This metabolic versatility facilitates interactions with a variable set of community members.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 714 
KW  - community
KW  - interactions
KW  - metagenomics
KW  - microbial ecology
KW  - mixed cultures
KW  - promiscuous
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-427299
IS  - 714
ER  - 
TY  - JOUR
A1  - Garcia, Sarahi L.
A1  - Buck, Moritz
A1  - McMahon, Katherine D.
A1  - Grossart, Hans-Peter
A1  - Eiler, Alexander
A1  - Warnecke, Falk
T1  - Auxotrophy and intrapopulation complementary in the "interactome' of a cultivated freshwater model community
JF  - Molecular ecology
N2  - Microorganisms are usually studied either in highly complex natural communities or in isolation as monoclonal model populations that we manage to grow in the laboratory. Here, we uncover the biology of some of the most common and yet-uncultured bacteria in freshwater environments using a mixed culture from Lake Grosse Fuchskuhle. From a single shotgun metagenome of a freshwater mixed culture of low complexity, we recovered four high-quality metagenome-assembled genomes (MAGs) for metabolic reconstruction. This analysis revealed the metabolic interconnectedness and niche partitioning of these naturally dominant bacteria. In particular, vitamin- and amino acid biosynthetic pathways were distributed unequally with a member of Crenarchaeota most likely being the sole producer of vitamin B12 in the mixed culture. Using coverage-based partitioning of the genes recovered from a single MAG intrapopulation metabolic complementarity was revealed pointing to social' interactions for the common good of populations dominating freshwater plankton. As such, our MAGs highlight the power of mixed cultures to extract naturally occurring interactomes' and to overcome our inability to isolate and grow the microbes dominating in nature.
KW  - community
KW  - cultures
KW  - interactions
KW  - metagenomics
KW  - populations
Y1  - 2015
U6  - https://doi.org/10.1111/mec.13319
SN  - 0962-1083
SN  - 1365-294X
VL  - 24
IS  - 17
SP  - 4449
EP  - 4459
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Garcia, Sarahi L.
A1  - Buck, Moritz
A1  - Hamilton, Joshua J.
A1  - Wurzbacher, Christian
A1  - Grossart, Hans-Peter
A1  - McMahon, Katherine D.
A1  - Eiler, Alexander
T1  - Model communities hint at promiscuous metabolic linkages between ubiquitous free-living freshwater bacteria
JF  - mSphere
N2  - Genome streamlining is frequently observed in free-living aquatic microorganisms and results in physiological dependencies between microorganisms. However, we know little about the specificity of these microbial associations. In order to examine the specificity and extent of these associations, we established mixed cultures from three different freshwater environments and analyzed the cooccurrence of organisms using a metagenomic time series. Free-living microorganisms with streamlined genomes lacking multiple biosynthetic pathways showed no clear recurring pattern in their interaction partners. Free-living freshwater bacteria form promiscuous cooperative associations. This notion contrasts with the well-documented high specificities of interaction partners in host-associated bacteria. Considering all data together, we suggest that highly abundant free-living bacterial lineages are functionally versatile in their interactions despite their distinct streamlining tendencies at the single-cell level. This metabolic versatility facilitates interactions with a variable set of community members.
KW  - community
KW  - interactions
KW  - metagenomics
KW  - microbial ecology
KW  - mixed cultures
KW  - promiscuous
Y1  - 2018
U6  - https://doi.org/10.1128/mSphere.00202-18
SN  - 2379-5042
VL  - 3
IS  - 3
PB  - American Society for Microbiology
CY  - Washington
ER  -