TY  - JOUR
A1  - Mazumder, Mostafizur
A1  - Brechun, Katherine E.
A1  - Kim, Yongjoo B.
A1  - Hoffmann, Stefan A.
A1  - Chen, Yih Yang
A1  - Keiski, Carrie-Lynn
A1  - Arndt, Katja Maren
A1  - McMillen, David R.
A1  - Woolley, G. Andrew
T1  - An Escherichia coli system for evolving improved light-controlled DNA-binding proteins
JF  - Protein engineering design & selection
N2  - Light-switchable proteins offer numerous opportunities as tools for manipulating biological systems with exceptional degrees of spatiotemporal control. Most designed light-switchable proteins currently in use have not been optimised using the randomisation and selection/screening approaches that are widely used in other areas of protein engineering. Here we report an approach for screening light-switchable DNA-binding proteins that relies on light-dependent repression of the transcription of a fluorescent reporter. We demonstrate that the method can be used to recover a known light-switchable DNA-binding protein from a random library.
KW  - directed evolution
KW  - fluorescent reporter
KW  - optogenetics
Y1  - 2015
U6  - https://doi.org/10.1093/protein/gzv033
SN  - 1741-0126
SN  - 1741-0134
VL  - 28
IS  - 9
SP  - 293
EP  - 302
PB  - Oxford Univ. Press
CY  - Oxford
ER  -