TY  - JOUR
A1  - Schenk, Jörg A.
A1  - Fettke, Jörg
A1  - Lenz, Christine
A1  - Albers, Katharina
A1  - Mallwitz, Frank
A1  - Gajovic-Eichelmann, Nenad
A1  - Ehrentreich-Förster, Eva
A1  - Kusch, Emely
A1  - Sellrie, Frank
T1  - Secretory leukocyte protease inhibitor (SLPI) might contaminate murine monoclonal antibodies after purification on protein G
JF  - Journal of biotechnology
N2  - The large scale production of a monoclonal anti-progesterone antibody in serum free medium followed by affinity chromatography on protein G lead to a contamination of the antibody sample with a protein of about 14 kDa. This protein was identified by mass spectrometry as secretory leukocyte protease inhibitor (SLPI). This SLPI contamination lead to a failure of the fiber-optic based competitive fluorescence assay to detect progesterone in milk. Purification of the monoclonal antibody using protein A columns circumvented this problem.
KW  - Hybridoma
KW  - SLPI
KW  - Protein G
KW  - Progesterone
KW  - Serum-free
Y1  - 2012
U6  - https://doi.org/10.1016/j.jbiotec.2011.12.025
SN  - 0168-1656
VL  - 158
IS  - 1-2
SP  - 34
EP  - 35
PB  - Elsevier
CY  - Amsterdam
ER  -