TY  - GEN
A1  - Lou, Ying
A1  - Ma, Hui
A1  - Lin, Wen-Hui
A1  - Chu, Zhao-Quing
A1  - Müller-Röber, Bernd
A1  - Xu, Zhi-Hong
A1  - Xue, Hong-Wei
T1  - The highly charged region of plant beta-type phosphatidylinositol 4-kinase is involved in membrane targeting and phospholipid binding
N2  - In Arabidopsis thaliana and Oryza sativa, two types of PI 4-kinase (PI4Ks) have been isolated and functionally characterized. The alpha-type PI4Ks (similar to 220 kDa) contain a PH domain, which is lacking in beta-type PI4Ks (similar to 120 kDa). beta-Type PI4Ks, exemplified by Arabidopsis AtPI4K beta and rice OsPI4K2, contain a highly charged repetitive segment designated PPC (Plant PI4K Charged) region, which is an unique domain only found in plant beta-type PI4Ks at present. The PPC region has a length of similar to 300 amino acids and harboring 11 (AtPI4K beta) and 14 (OsPI4K2) repeats, respectively, of a 20-aa motif. Studies employing a modified yeast-based "Sequence of Membrane- Targeting Detection'' system demonstrate that the PPC(OsPI4K2) region, as well as the former 8 and latter 6 repetitive motifs within the PPC region, are able to target fusion proteins to the plasma membrane. Further detection on the transiently expressed GFP fusion proteins in onion epidermal cells showed that the PPC(OsPI4K2) region alone, as well as the region containing repetitive motifs 1-8, was able to direct GFP to the plasma membrane, while the regions containing less repetitive motifs, i.e. 6, 4, 2 or single motif(s) led to predominantly intracellular localization. Agrobacterium-mediated transient expression of PPC-GFP fusion protein further confirms the membrane-targeting capacities of PPC region. In addition, the predominant plasma membrane localization of AtPI4Kb was mediated by the PPC region. Recombinant PPC peptide, expressed in E. coli, strongly binds phosphatidic acid, PI and PI4P, but not phosphatidylcholine, PI5P, or PI(4,5) P-2 in vitro, providing insights into potential mechanisms for regulating sub- cellular localization and lipid binding for the plant beta-type PI4Ks
Y1  - 2006
UR  - http://www.springerlink.com/content/100330
U6  - https://doi.org/10.1007/s11103-005-5548-x
SN  - 0167-4412
ER  - 
TY  - GEN
A1  - Köchy, Martin
T1  - Photodegradation of grass litter in semi-arid grasslands : a global perspective
N2  - In a recent contribution in Nature (vol. 442, pp. 555-558) Austin & Vivanco showed that sunlight is the dominant factor for decomposition of grass litter in a semi-arid grassland in Argentine. The quantification of this effect was portrayed as a novel finding. I put this result in the context of three other publications from as early as 1980 that quantified photodegradation. My synopsis shows that photodegradation is an important process in semi-arid grasslands in South America, North America and eastern Europe.
KW  - Laubstreu
KW  - semi-arides Grasland
KW  - abiotische Zersetzung
KW  - UV-Licht
KW  - Schatten
KW  - leaf litter
KW  - semi-arid grassland
KW  - abiotic decomposition
KW  - UV radiation
KW  - shade
Y1  - 2006
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-12006
ER  -