TY  - JOUR
A1  - Balazadeh, Salma
A1  - Siddiqui, Hamad
A1  - Allu, Annapurna Devi
A1  - Matallana-Ramirez, Lilian Paola
A1  - Caldana, Camila
A1  - Mehrnia, Mohammad
A1  - Zanor, Maria-Inés
A1  - Koehler, Barbara
A1  - Müller-Röber, Bernd
T1  - A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence
N2  - P>The onset and progression of senescence are under genetic and environmental control. The Arabidopsis thaliana NAC transcription factor ANAC092 (also called AtNAC2 and ORE1) has recently been shown to control age-dependent senescence, but its mode of action has not been analysed yet. To explore the regulatory network administered by ANAC092 we performed microarray-based expression profiling using estradiol-inducible ANAC092 overexpression lines. Approximately 46% of the 170 genes up-regulated upon ANAC092 induction are known senescence-associated genes, suggesting that the NAC factor exerts its role in senescence through a regulatory network that includes many of the genes previously reported to be senescence regulated. We selected 39 candidate genes and confirmed their time-dependent response to enhanced ANAC092 expression by quantitative RT-PCR. We also found that the majority of them (24 genes) are up-regulated by salt stress, a major promoter of plant senescence, in a manner similar to that of ANAC092, which itself is salt responsive. Furthermore, 24 genes like ANAC092 turned out to be stage-dependently expressed during seed growth with low expression at early and elevated expression at late stages of seed development. Disruption of ANAC092 increased the rate of seed germination under saline conditions, whereas the opposite occurred in respective overexpression plants. We also detected a delay of salinity-induced chlorophyll loss in detached anac092-1 mutant leaves. Promoter-reporter (GUS) studies revealed transcriptional control of ANAC092 expression during leaf and flower ageing and in response to salt stress. We conclude that ANAC092 exerts its functions during senescence and seed germination through partly overlapping target gene sets.
Y1  - 2010
UR  - http://www3.interscience.wiley.com/cgi-bin/issn?DESCRIPTOR=PRINTISSN&VALUE=0960-7412
U6  - https://doi.org/10.1111/j.1365-313X.2010.04151.x
SN  - 0960-7412
ER  - 
TY  - JOUR
A1  - Gliwicka, Marta
A1  - Balazadeh, Salma
A1  - Caldana, Camila
A1  - Müller-Röber, Bernd
A1  - Gaj, Malgorzata D.
T1  - The use of multi-qPCR platform and tan1 mutant in identification of TF genes involved in somatic embryogenesis in Arabidopsis
Y1  - 2009
UR  - http://www.ib.uj.edu.pl/abc/index.php?d=06
SN  - 0001-5296
ER  - 
TY  - THES
A1  - Balazadeh, Salma
T1  - Molecular and physiological analysis of leaf senescence in Arabidopsis thaliana
Y1  - 2008
ER  - 
TY  - THES
A1  - Balazadeh, Salma
T1  - New insights into the molecular mechanisms of leaf senescence
Y1  - 2015
ER  - 
TY  - GEN
A1  - Szarzynska, Bogna
A1  - Sobkowiak, Lukasz
A1  - Pant, Bikram Datt
A1  - Balazadeh, Salma
A1  - Scheible, Wolf-Rüdiger
A1  - Müller-Röber, Bernd
A1  - Jarmolowski, Artur
A1  - Szweykowska-Kulinska, Zofia
T1  - Gene structures and processing of Arabidopsis thaliana HYL1-dependent pri-miRNAs
N2  - Arabidopsis thaliana HYL1 is a nuclear doublestranded RNA-binding protein involved in the maturation of pri-miRNAs. A quantitative real-time PCR platform for parallel quantification of 176 primiRNAs was used to reveal strong accumulation of 57 miRNA precursors in the hyl1 mutant that completely lacks HYL1 protein. This approach enabled us for the first time to pinpoint particular members of MIRNA family genes that require HYL1 activity for efficient maturation of their precursors. Moreover, the accumulation of miRNA precursors in the hyl1 mutant gave us the opportunity to carry out 3’ and 5’ RACE experiments which revealed that some of these precursors are of unexpected length. The alignment of HYL1- dependent miRNA precursors to A. thaliana genomic sequences indicated the presence of introns in 12 out of 20 genes studied. Some of the characterized intron-containing pri-miRNAs undergo alternative splicing such as exon skipping or usage of alternative 5’ splice sites suggesting that this process plays a role in the regulation of miRNA biogenesis. In the hyl1 mutant intron-containing pri-miRNAs accumulate alongside spliced primiRNAs suggesting the recruitment of HYL1 into the miRNA precursor maturation pathway before their splicing occurs.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 138 
KW  - Binding-protein hyl1
KW  - Abscisic-acid
KW  - Flowering time
KW  - Micro-RNA
KW  - Serrate
Y1  - 2009
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45085
ER  -