TY  - JOUR
A1  - Lisdat, Fred
A1  - Wollenberger, Ursula
A1  - Paeschke, Manfred
A1  - Scheller, Frieder W.
T1  - Sensitive catecholamine measurement using a monoenzymatic recycling system
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Lisdat, Fred
A1  - Dronov, Roman
A1  - Möhwald, Helmuth
A1  - Scheller, Frieder W.
A1  - Kurth, Dirk G.
T1  - Self-assembly of electro-active protein architectures on electrodes for the construction of biomimetic signal chains
N2  - The layer-by-layer adsorption technique based on the consecutive deposition of oppositely charged species is for the preparation of protein multilayers with fully electro-active protein molecules. The methodology was established with cytochrome c and the polyelectrolyte sulfonated polyaniline (PASA). The technique is also useful for the construction of bi-protein architectures confining protein-protein communication to an electrode. Following natural examples of protein complexes with defined signal transfer, cytochrome c was arranged with enzymes such as xanthine oxidase, bilirubin oxidase, laccase, and sulfite oxidase in self-assembled multilayer architectures. Thus, biomimetic signal chains from the enzyme substrate via the enzyme and cytochrome c towards the electrode can be established. Communication between proteins immobilised in multiple layers on the electrode can be achieved by in situ generation of small shuttle molecules or more advantageously by direct interprotein electron transfer. This allows the construction of new sensing electrodes, the properties of which can be tuned by the number of deposited protein layers. The mechanism of electron transfer within such protein assemblies on gold electrodes will be discussed.
Y1  - 2009
UR  - http://xlink.rsc.org/jumptojournal.cfm?journal_code=CC
U6  - https://doi.org/10.1039/B813559b
SN  - 1359-7345
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Wollenberger, Ursula
A1  - Warsinke, Axel
A1  - Lisdat, Fred
T1  - Research and development in biosensors
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Lisdat, Fred
A1  - Ho, Wah O.
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
A1  - Richter, Torsten
A1  - Bilitewski, Ursula
T1  - Recycling systems based on screen-printed electrodes
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Schubert, Florian
A1  - Pfeiffer, Dorothea
A1  - Scheller, Frieder W.
T1  - Recycling sensors based on kinases : proceedings of Mosbach Symposion on Biochemical Technology
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Kleinjung, Frank
A1  - Scheller, Frieder W.
T1  - Real time measurement of nucleic acid hybridization using evanescent wave sensors - step towards the genosensor
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Welzel, H.-P.
A1  - Kossmehl, G.
A1  - Engelmann, G.
A1  - Neumann, B.
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
A1  - Schröder, W.
T1  - Reactive groups on polymer covered electrodes, 4. Lactate-oxidase-biosensor based on electrodes modifies by polyphiophene
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Rose, Andreas
A1  - Pfeiffer, Dorothea
A1  - Scheller, Frieder W.
A1  - Wollenberger, Ursula
T1  - Quinoprotein glucose dehydrogenasemodified thick-film electrodes for the amperometric detection of phenolic compounds in flow injection analysis
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Lisdat, Fred
A1  - Scheller, Frieder W.
T1  - Principles of sensorial radical detection - a minireview
Y1  - 2000
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Schubert, Frank
A1  - Federowitz, J.
T1  - Present state and frontiers in biosensorics
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Heiduschka, P.
T1  - Preparation of an electrode surface with a high density of binding sites by an electrochemical reduction of a poly (nitrophenol) film
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Jin, Wen
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - PQQ as redox shuttle for quinoprotein glucose dehydrogenase
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Ghindilis, A. L.
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Potentiometric enzyme electrodes based on substrate recycling and mediatorless bioelectrocatalysis
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Halamek, Jan
A1  - Makower, Alexander
A1  - Knösche, Kristina
A1  - Skladal, Petr
A1  - Scheller, Frieder W.
T1  - Piezoelectric affinity sensors for cocaine and cholinesterase inhibitors
N2  - We report here the development of piezoelectric affinity sensors for cocaine and cholinesterase inhibitors based on the formation of affinity complexes between an immobilized cocaine derivative and an anti-cocaine antibody or cholinesterase. For both binding reactions benzoylecgonine-1,8-diamino-3,4-dioxaoctane (BZE-DADOO) was immobilized on the surface of the sensor. For immobilization. pre-conjugated BZE-DADOO with 11-mercaptomonoundecanoic acid (MUA) via 2- (5-norbornen-2,3-dicarboximide)-1,1,3,3-tetramethyluronium-tetrafluoro borate (TNTU) allowed the formation of a chemisorbed monolayer on the piezosensor surface. The detection of cocaine was based oil a competitive assay. The change of frequency measured after 300 s of the binding reaction was used as the signal. The maximum binding of the antibody resulted in a frequency decrease of 35 Hz (with an imprecision 3%, n = 3) while the presence of 100 pmol I-1 cocaine decreased the binding by 11%. The limit of detection was consequently below 100 pmol I-1 for cocaine. The total time of one analysis was 15 min. This BZE-DADOO-modified sensor was adapted for the detection of organophosphates. BZE-DADOO - a competitive inhibitor - served as binding element for cholinesterase in a competitive assay. (C) 2004 Elsevier B.V. All rights reserved
Y1  - 2005
ER  - 
TY  - JOUR
A1  - Bognár, Zsófia
A1  - Supala, Eszter
A1  - Yarman, Aysu
A1  - Zhang, Xiaorong
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
A1  - Gyurcsanyi, Róbert E.
T1  - Peptide epitope-imprinted polymer microarrays for selective protein recognition
BT  - application for SARS-CoV-2 RBD protein
JF  - Chemical science / RSC, Royal Society of Chemistry
N2  - We introduce a practically generic approach for the generation of epitope-imprinted polymer-based microarrays for protein recognition on surface plasmon resonance imaging (SPRi) chips. The SPRi platform allows the subsequent rapid screening of target binding kinetics in a multiplexed and label-free manner. The versatility of such microarrays, both as synthetic and screening platform, is demonstrated through developing highly affine molecularly imprinted polymers (MIPs) for the recognition of the receptor binding domain (RBD) of SARS-CoV-2 spike protein. A characteristic nonapeptide GFNCYFPLQ from the RBD and other control peptides were microspotted onto gold SPRi chips followed by the electrosynthesis of a polyscopoletin nanofilm to generate in one step MIP arrays. A single chip screening of essential synthesis parameters, including the surface density of the template peptide and its sequence led to MIPs with dissociation constants (K-D) in the lower nanomolar range for RBD, which exceeds the affinity of RBD for its natural target, angiotensin-convertase 2 enzyme. Remarkably, the same MIPs bound SARS-CoV-2 virus like particles with even higher affinity along with excellent discrimination of influenza A (H3N2) virus. While MIPs prepared with a truncated heptapeptide template GFNCYFP showed only a slightly decreased affinity for RBD, a single mismatch in the amino acid sequence of the template, i.e. the substitution of the central cysteine with a serine, fully suppressed the RBD binding.
Y1  - 2021
U6  - https://doi.org/10.1039/d1sc04502d
SN  - 2041-6539
VL  - 13
IS  - 5
SP  - 1263
EP  - 1269
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Kleuser, U.
A1  - Stöcklein, Walter F. M.
A1  - Pieper-Fürst, U.
A1  - Scheller, Frieder W.
T1  - Partikelverstärkte Oberflächenplasmonresonanz für die Quantifizierung von Matrix Metalloproteinase-2
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Wollenberger, Ursula
A1  - Pfeiffer, Dorothea
A1  - Schubert, Florian
T1  - Overview of biosensor technology : proceedings of Mosbach Symposion on Biochemical Technology
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - Organic solvent modified enzyme-liked immunoassay for the detection of triazine herbicides
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Gajovic, Nenad
A1  - Binyamin, Gary
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
A1  - Heller, A.
T1  - Operation of a miniature redox hydrogel-based pyruvate sensor in undiluted deoxygenated calf serum
Y1  - 2000
ER  - 
TY  - JOUR
A1  - Nitsche, Andreas
A1  - Kurth, Andreas
A1  - Dunkhorst, Anna
A1  - Pänke, Oliver
A1  - Sielaff, Hendrik
A1  - Junge, Wolfgang
A1  - Muth, Doreen
A1  - Scheller, Frieder W.
A1  - Stöcklein, Walter F. M.
A1  - Pauli, Georg
A1  - Kage, Andreas
T1  - One-step selection of vaccinia virus binding DNA-aptamers by MonoLEX
Y1  - 2007
UR  - http://www.biomedcentral.com/1472-6750/7
U6  - https://doi.org/10.1186/1472-6750-7-48
ER  - 
TY  - JOUR
A1  - Lisdat, Fred
A1  - Ge, Bixia
A1  - Scheller, Frieder W.
T1  - Oligonucleotide-modified electrodes for fast electron transfer to cytochrome c
Y1  - 1999
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Fürste, J. P.
A1  - Kleinjung, Frank
A1  - Erdmann, V. A.
A1  - Scheller, Frieder W.
T1  - Nukleinsäuren als Basis für Biosensoren
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Lisdat, Fred
A1  - Ge, Bixia
A1  - Krause, B.
A1  - Ehrlich, A.
A1  - Bienert, H.
A1  - Scheller, Frieder W.
T1  - Nucleic acid-promoted electron transfer to cytochrome c
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Freaney, R.
A1  - MacShane, A.
A1  - Keaveny, T. V.
A1  - MacKenna, M.
A1  - Rabenstein, K.
A1  - Scheller, Frieder W.
A1  - Pfeiffer, Dorothea
A1  - Urban, G.
A1  - Moser, I.
A1  - Jobst, G.
A1  - Manz, A.
A1  - Verpoorte, E.
A1  - Widmer, M. W.
A1  - Diamond, D.
A1  - Dempsey, E.
A1  - deViteri, F. J. S.
A1  - Smyth, M.
T1  - Novel instrumentation for real-time monitoring using miniaturized flow systems with integrated biosensors
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Kleinjung, Frank
A1  - Klußmann, S.
A1  - Erdmann, V. A.
A1  - Scheller, Frieder W.
A1  - Fürste, J. P.
A1  - Bier, Frank Fabian
T1  - Novel binders in biosensorics : hight affinity RNA for smal analytes
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Kleinjung, Frank
A1  - Bier, Frank Fabian
A1  - Markower, Alexander
A1  - Neumann, Barbara
A1  - Wollenberger, Ursula
A1  - Kurochkin, Iliya N.
A1  - Eremenko, Arkadi V.
A1  - Barmin, Anatoli V.
A1  - Klußmann, Sven
A1  - Fürste, Jens-Peter
A1  - Erdmann, Volker A.
A1  - Mansuy, D.
T1  - New recognition elements in biosensing
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
T1  - New recognition elements for bioanalytics
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Makower, Alexander
A1  - Halámek, Jan
A1  - Skládal, Petr
A1  - Kernchen, Frank
A1  - Scheller, Frieder W.
T1  - New principle of direct real-time monitoring of the interaction of cholinesterase and its inhibitors by piezoelectric biosensor
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Bauer, Christian G.
A1  - Kühn, A.
A1  - Gajovic, Nenad
A1  - Skorobogatko, O. V.
A1  - Holt, P. J.
A1  - Bruce, N. C.
A1  - Makower, Alexander
A1  - Lowe, Ch. R.
A1  - Scheller, Frieder W.
T1  - New enzymen sensors for morphine and codeine based on morphine dehydrogenase and laccase
Y1  - 1999
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
T1  - Neue Dimensionen der Biosensorik
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Ghindilis, A. L.
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Nanomolar determination of the ferrocene derivatives using a recycling enzyme electrode : development of the redox label immunoassay
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Schmidt, Peter Michael
A1  - Matthes, E.
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
T1  - Nachweis der Telomeraseaktivität in Zellkulturen mittels eines faseroptischen Sensors
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Kröning, Steffen
A1  - Scheller, Frieder W.
A1  - Wollenberger, Ursula
A1  - Lisdat, Fred
T1  - Myoglobin-Clay Electrode for Nitric Oxide (NO) Detection in Solution
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Wollenberger, Ursula
A1  - Schubert, Florian
A1  - Pfeiffer, Dorothea
A1  - Markower, Alexander
A1  - McNeil, C. J.
T1  - Multienzyme biosensors : coupled enzyme reactions and enzyme activation
Y1  - 1993
ER  - 
TY  - JOUR
A1  - Peng, Lei
A1  - Yarman, Aysu
A1  - Jetzschmann, Katharina J.
A1  - Jeoung, Jae-Hun
A1  - Schad, Daniel
A1  - Dobbek, Holger
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Molecularly Imprinted Electropolymer for a Hexameric Heme Protein with Direct Electron Transfer and Peroxide Electrocatalysis
JF  - SENSORS
N2  - For the first time a molecularly imprinted polymer (MIP) with direct electron transfer (DET) and bioelectrocatalytic activity of the target protein is presented. Thin films of MIPs for the recognition of a hexameric tyrosine-coordinated heme protein (HTHP) have been prepared by electropolymerization of scopoletin after oriented assembly of HTHP on a self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) on gold electrodes. Cavities which should resemble the shape and size of HTHP were formed by template removal. Rebinding of the target protein sums up the recognition by non-covalent interactions between the protein and the MIP with the electrostatic attraction of the protein by the SAM. HTHP bound to the MIP exhibits quasi-reversible DET which is reflected by a pair of well pronounced redox peaks in the cyclic voltammograms (CVs) with a formal potential of -184.4 +/- 13.7 mV vs. Ag/AgCl (1 M KCl) at pH 8.0 and it was able to catalyze the cathodic reduction of peroxide. At saturation the MIP films show a 12-fold higher electroactive surface concentration of HTHP than the non-imprinted polymer (NIP).
KW  - hydrogen peroxide
KW  - bioelectrocatalysis
KW  - molecularly imprinted polymers
KW  - direct electron transfer
KW  - self-assembled monolayer
Y1  - 2016
U6  - https://doi.org/10.3390/s16030272
SN  - 1424-8220
VL  - 16
SP  - 1343
EP  - 1364
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Knösche, Kristina
A1  - Halámek, Jan
A1  - Makower, Alexander
A1  - Fournier, Didier
A1  - Scheller, Frieder W.
T1  - Molecular recognition of cocaine by acetylcholinesterases for affinity purification and bio-sensing
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Jetzschmann, Katharina J.
A1  - Yarman, Aysu
A1  - Rustam, L.
A1  - Kielb, P.
A1  - Urlacher, V. B.
A1  - Fischer, A.
A1  - Weidinger, I. M.
A1  - Wollenberger, Ulla
A1  - Scheller, Frieder W.
T1  - Molecular LEGO by domain-imprinting of cytochrome P450 BM3
JF  - Colloids and surfaces : an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin ; B, Biointerfaces
N2  - Hypothesis: Electrosynthesis of the MIP nano-film after binding of the separated domains or holocytochrome BM3 via an engineered anchor should result in domain-specific cavities in the polymer layer. Experiments: Both the two domains and the holo P450 BM3 have been bound prior polymer deposition via a N-terminal engineered his6-anchor to the electrode surface. Each step of MIP preparation was characterized by cyclic voltammetry of the redox-marker ferricyanide. Rebinding after template removal was evaluated by quantifying the suppression of the diffusive permeability of the signal for ferricyanide and by the NADH-dependent reduction of cytochrome c by the reductase domain (BMR). Findings: The working hypothesis is verified by the discrimination of the two domains by the respective MIPs: The holoenzyme P450 BM3 was ca. 5.5 times more effectively recognized by the film imprinted with the oxidase domain (BMO) as compared to the BMR-MIP or the non-imprinted polymer (NIP). Obviously, a cavity is formed during the imprinting process around the hiss-tag-anchored BMR which cannot accommodate the broader BMO or the P450 BM3. The affinity of the MIP towards P450 BM3 is comparable with that to the monomer in solution. The hiss-tagged P450 BM3 binds (30 percent) stronger which shows the additive effect of the interaction with the MIP and the binding to the electrode.
KW  - Molecularly imprinted polymers
KW  - Protein imprinting
KW  - Electropolymerization
KW  - Cytochrome P450
Y1  - 2018
U6  - https://doi.org/10.1016/j.colsurfb.2018.01.047
SN  - 0927-7765
SN  - 1873-4367
VL  - 164
SP  - 240
EP  - 246
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Bosserdt, Maria
A1  - Gajovic-Eichelman, Nenad
A1  - Scheller, Frieder W.
T1  - Modulation of direct electron transfer of cytochrome c by use of a molecularly imprinted thin film
JF  - Analytical & bioanalytical chemistry
N2  - We describe the preparation of a molecularly imprinted polymer film (MIP) on top of a self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) on gold, where the template cytochrome c (cyt c) participates in direct electron transfer (DET) with the underlying electrode. To enable DET, a non-conductive polymer film is electrodeposited from an aqueous solution of scopoletin and cyt c on to the surface of a gold electrode previously modified with MUA. The electroactive surface concentration of cyt c was 0.5 pmol cm(-2). In the absence of the MUA layer, no cyt c DET was observed and the pseudo-peroxidatic activity of the scopoletin-entrapped protein, assessed via oxidation of Ampliflu red in the presence of hydrogen peroxide, was only 30 % of that for the MIP on MUA. This result indicates that electrostatic adsorption of cyt c by the MUA-SAM substantially increases the surface concentration of cyt c during the electrodeposition step, and is a prerequisite for the productive orientation required for DET. After template removal by treatment with sulfuric acid, rebinding of cyt c to the MUA-MIP-modified electrode occurred with an affinity constant of 100,000 mol(-1) L, a value three times higher than that determined by use of fluorescence titration for the interaction between scopoletin and cyt c in solution. The DET of cyt c in the presence of myoglobin, lysozyme, and bovine serum albumin (BSA) reveals that the MIP layer suppresses the effect of competing proteins.
KW  - Cytochrome c
KW  - Molecularly imprinted polymer film
KW  - Mercaptoundecanoic acid
KW  - Direct electron transfer
KW  - Scopoletin (7-hydroxy-6-methoxycoumarin)
Y1  - 2013
U6  - https://doi.org/10.1007/s00216-013-7009-8
SN  - 1618-2642
VL  - 405
IS  - 20
SP  - 6437
EP  - 6444
PB  - Springer
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Lehmann, Claudia
A1  - Wollenberger, Ursula
A1  - Brigelius-Flohé, Regina
A1  - Scheller, Frieder W.
T1  - Modified gold electrodes for electrochemical studies of the reaction phospholipid hydroperoxide glutathione peroxidas with glutathione and glutathione disulfide
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Yarman, Aysu
A1  - Scheller, Frieder W.
T1  - MIP-esterase/Tyrosinase Combinations for Paracetamol and Phenacetin
JF  - Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis
N2  - A new electrochemical MIP sensor for the most frequently used drug paracetamol (PAR) was prepared by electropolymerization of mixtures containing the template molecule and the functional monomers ophenylenediamine, resorcinol and aniline. The imprinting factor of 12 reflects the effective target binding to the MIP as compared with the non-imprinted electropolymer. Combination of the MIP with a nonspecific esterase allows the measurement of phenacetin - another analgesic drug. In the second approach the PAR containing sample solution was pretreated with tyrosinase in order to prevent electrochemical interferences by ascorbic acid and uric acid. Interference-free indication at a very low electrode potential without fouling of the electrode surface was achieved with the o-phenylenediamine: resorcinol-based MIP.
KW  - Paracetamol
KW  - Molecularly imprinted polymers
KW  - Electropolymerization
KW  - Tyrosinase
KW  - Esterase
KW  - Phenacetin
Y1  - 2016
U6  - https://doi.org/10.1002/elan.201600042
SN  - 1040-0397
SN  - 1521-4109
VL  - 28
SP  - 2222
EP  - 2227
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Riedel, K.
A1  - Beyersdorf-Radeck, Baerbel
A1  - Neumann, B.
A1  - Scheller, Frieder W.
A1  - Schmid, Rolf D.
T1  - Microbial sensors for determination of aromatics and their chloro derivatives. Part III: Determination of chlorinated phenols using a biosensor containing Trichosporon beigelii (cutaneum)
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Kleinjung, Frank
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Messungen an Nukleinsäuren mittels evaneszenten Feldes
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Frasca, Stefano
A1  - von Graberg, Till
A1  - Feng, Jiu-Ju
A1  - Thomas, Arne
A1  - Smarsly, Bernd M.
A1  - Weidinger, Inez M.
A1  - Scheller, Frieder W.
A1  - Hildebrandt, Peter
A1  - Wollenberger, Ursula
T1  - Mesoporous indium tin oxide as a novel platform for bioelectronics
N2  - Stable immobilization and reversible electrochemistry of cytochrome c in a tranparent indium tin oxide film with a well-defined mesoporosity (mpITO) is demonstrated. the transparency and good conductivity, in combination with the large surface area of mpITO, allow the incorporation of a high amount of elelctroactive biomolecules and their electrochemical and spectroscopic investigation. UV/Vis and resonance Raman spectroscopy, in combination with direct protein voltammetry are employed for the characterization of cytochrome c immobilized in the mpITO and reveal no perturbant of the structural of the integrity of the redox protein. The potential of this modified material as a biosensor detection of superoxide anions is also demonstrated.
Y1  - 2010
UR  - http://www3.interscience.wiley.com/journal/122208635/home
U6  - https://doi.org/10.1002/cctc.201000047
SN  - 1867-3880
ER  - 
TY  - JOUR
A1  - Stöllner, Daniela
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
A1  - Warsinke, Axel
T1  - Membrane-immobilized haptoglobin as affinity matrix for a hemoglobin-A1c-immunosensor
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Kulys, J.
A1  - Drungiliene, A.
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Membrane covered carbon paste electrode for the electrochemical determination of perioxidase and microperoxidase in a flow system
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Eremenko, A. V.
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Measurement of nanomolar diphenols by substrate recycling coupled to a pH- sensitive electrode
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Schulmeister, Thomas
A1  - Rose, Jürgen
A1  - Scheller, Frieder W.
T1  - Mathematical modelling of exponential amplification in membrane-based enzyme sensors
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
T1  - Laccase : a marker enzyme for solvent modified immunoassays
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Ghindilis, A. L.
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Laccase - glucose dehydrogenase recycling enzyme electrode based on potentiometric mediatorless electrocatalytic detection
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Label-free observation of DNA-hybridisation and endonuclease activity on a wave guide surface using a grating coupler
Y1  - 1996
ER  -