TY - JOUR A1 - Lisdat, Fred A1 - Wollenberger, Ursula A1 - Paeschke, Manfred A1 - Scheller, Frieder W. T1 - Sensitive catecholamine measurement using a monoenzymatic recycling system Y1 - 1998 ER - TY - JOUR A1 - Lisdat, Fred A1 - Dronov, Roman A1 - Möhwald, Helmuth A1 - Scheller, Frieder W. A1 - Kurth, Dirk G. T1 - Self-assembly of electro-active protein architectures on electrodes for the construction of biomimetic signal chains N2 - The layer-by-layer adsorption technique based on the consecutive deposition of oppositely charged species is for the preparation of protein multilayers with fully electro-active protein molecules. The methodology was established with cytochrome c and the polyelectrolyte sulfonated polyaniline (PASA). The technique is also useful for the construction of bi-protein architectures confining protein-protein communication to an electrode. Following natural examples of protein complexes with defined signal transfer, cytochrome c was arranged with enzymes such as xanthine oxidase, bilirubin oxidase, laccase, and sulfite oxidase in self-assembled multilayer architectures. Thus, biomimetic signal chains from the enzyme substrate via the enzyme and cytochrome c towards the electrode can be established. Communication between proteins immobilised in multiple layers on the electrode can be achieved by in situ generation of small shuttle molecules or more advantageously by direct interprotein electron transfer. This allows the construction of new sensing electrodes, the properties of which can be tuned by the number of deposited protein layers. The mechanism of electron transfer within such protein assemblies on gold electrodes will be discussed. Y1 - 2009 UR - http://xlink.rsc.org/jumptojournal.cfm?journal_code=CC U6 - https://doi.org/10.1039/B813559b SN - 1359-7345 ER - TY - JOUR A1 - Scheller, Frieder W. A1 - Wollenberger, Ursula A1 - Warsinke, Axel A1 - Lisdat, Fred T1 - Research and development in biosensors Y1 - 2001 ER - TY - JOUR A1 - Lisdat, Fred A1 - Ho, Wah O. A1 - Wollenberger, Ursula A1 - Scheller, Frieder W. A1 - Richter, Torsten A1 - Bilitewski, Ursula T1 - Recycling systems based on screen-printed electrodes Y1 - 1998 ER - TY - JOUR A1 - Wollenberger, Ursula A1 - Schubert, Florian A1 - Pfeiffer, Dorothea A1 - Scheller, Frieder W. T1 - Recycling sensors based on kinases : proceedings of Mosbach Symposion on Biochemical Technology Y1 - 1996 ER - TY - JOUR A1 - Bier, Frank Fabian A1 - Kleinjung, Frank A1 - Scheller, Frieder W. T1 - Real time measurement of nucleic acid hybridization using evanescent wave sensors - step towards the genosensor Y1 - 1997 ER - TY - JOUR A1 - Welzel, H.-P. A1 - Kossmehl, G. A1 - Engelmann, G. A1 - Neumann, B. A1 - Wollenberger, Ursula A1 - Scheller, Frieder W. A1 - Schröder, W. T1 - Reactive groups on polymer covered electrodes, 4. Lactate-oxidase-biosensor based on electrodes modifies by polyphiophene Y1 - 1996 ER - TY - JOUR A1 - Rose, Andreas A1 - Pfeiffer, Dorothea A1 - Scheller, Frieder W. A1 - Wollenberger, Ursula T1 - Quinoprotein glucose dehydrogenasemodified thick-film electrodes for the amperometric detection of phenolic compounds in flow injection analysis Y1 - 2001 ER - TY - JOUR A1 - Lisdat, Fred A1 - Scheller, Frieder W. T1 - Principles of sensorial radical detection - a minireview Y1 - 2000 ER - TY - JOUR A1 - Scheller, Frieder W. A1 - Schubert, Frank A1 - Federowitz, J. T1 - Present state and frontiers in biosensorics Y1 - 1997 ER - TY - JOUR A1 - Scheller, Frieder W. A1 - Heiduschka, P. T1 - Preparation of an electrode surface with a high density of binding sites by an electrochemical reduction of a poly (nitrophenol) film Y1 - 1994 ER - TY - JOUR A1 - Jin, Wen A1 - Wollenberger, Ursula A1 - Scheller, Frieder W. T1 - PQQ as redox shuttle for quinoprotein glucose dehydrogenase Y1 - 1998 ER - TY - JOUR A1 - Ghindilis, A. L. A1 - Makower, Alexander A1 - Scheller, Frieder W. T1 - Potentiometric enzyme electrodes based on substrate recycling and mediatorless bioelectrocatalysis Y1 - 1995 ER - TY - JOUR A1 - Halamek, Jan A1 - Makower, Alexander A1 - Knösche, Kristina A1 - Skladal, Petr A1 - Scheller, Frieder W. T1 - Piezoelectric affinity sensors for cocaine and cholinesterase inhibitors N2 - We report here the development of piezoelectric affinity sensors for cocaine and cholinesterase inhibitors based on the formation of affinity complexes between an immobilized cocaine derivative and an anti-cocaine antibody or cholinesterase. For both binding reactions benzoylecgonine-1,8-diamino-3,4-dioxaoctane (BZE-DADOO) was immobilized on the surface of the sensor. For immobilization. pre-conjugated BZE-DADOO with 11-mercaptomonoundecanoic acid (MUA) via 2- (5-norbornen-2,3-dicarboximide)-1,1,3,3-tetramethyluronium-tetrafluoro borate (TNTU) allowed the formation of a chemisorbed monolayer on the piezosensor surface. The detection of cocaine was based oil a competitive assay. The change of frequency measured after 300 s of the binding reaction was used as the signal. The maximum binding of the antibody resulted in a frequency decrease of 35 Hz (with an imprecision 3%, n = 3) while the presence of 100 pmol I-1 cocaine decreased the binding by 11%. The limit of detection was consequently below 100 pmol I-1 for cocaine. The total time of one analysis was 15 min. This BZE-DADOO-modified sensor was adapted for the detection of organophosphates. BZE-DADOO - a competitive inhibitor - served as binding element for cholinesterase in a competitive assay. (C) 2004 Elsevier B.V. All rights reserved Y1 - 2005 ER - TY - JOUR A1 - Bognár, Zsófia A1 - Supala, Eszter A1 - Yarman, Aysu A1 - Zhang, Xiaorong A1 - Bier, Frank Fabian A1 - Scheller, Frieder W. A1 - Gyurcsanyi, Róbert E. T1 - Peptide epitope-imprinted polymer microarrays for selective protein recognition BT - application for SARS-CoV-2 RBD protein JF - Chemical science / RSC, Royal Society of Chemistry N2 - We introduce a practically generic approach for the generation of epitope-imprinted polymer-based microarrays for protein recognition on surface plasmon resonance imaging (SPRi) chips. The SPRi platform allows the subsequent rapid screening of target binding kinetics in a multiplexed and label-free manner. The versatility of such microarrays, both as synthetic and screening platform, is demonstrated through developing highly affine molecularly imprinted polymers (MIPs) for the recognition of the receptor binding domain (RBD) of SARS-CoV-2 spike protein. A characteristic nonapeptide GFNCYFPLQ from the RBD and other control peptides were microspotted onto gold SPRi chips followed by the electrosynthesis of a polyscopoletin nanofilm to generate in one step MIP arrays. A single chip screening of essential synthesis parameters, including the surface density of the template peptide and its sequence led to MIPs with dissociation constants (K-D) in the lower nanomolar range for RBD, which exceeds the affinity of RBD for its natural target, angiotensin-convertase 2 enzyme. Remarkably, the same MIPs bound SARS-CoV-2 virus like particles with even higher affinity along with excellent discrimination of influenza A (H3N2) virus. While MIPs prepared with a truncated heptapeptide template GFNCYFP showed only a slightly decreased affinity for RBD, a single mismatch in the amino acid sequence of the template, i.e. the substitution of the central cysteine with a serine, fully suppressed the RBD binding. Y1 - 2021 U6 - https://doi.org/10.1039/d1sc04502d SN - 2041-6539 VL - 13 IS - 5 SP - 1263 EP - 1269 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Kleuser, U. A1 - Stöcklein, Walter F. M. A1 - Pieper-Fürst, U. A1 - Scheller, Frieder W. T1 - Partikelverstärkte Oberflächenplasmonresonanz für die Quantifizierung von Matrix Metalloproteinase-2 Y1 - 2004 ER - TY - JOUR A1 - Scheller, Frieder W. A1 - Wollenberger, Ursula A1 - Pfeiffer, Dorothea A1 - Schubert, Florian T1 - Overview of biosensor technology : proceedings of Mosbach Symposion on Biochemical Technology Y1 - 1996 ER - TY - JOUR A1 - Stöcklein, Walter F. M. A1 - Warsinke, Axel A1 - Scheller, Frieder W. T1 - Organic solvent modified enzyme-liked immunoassay for the detection of triazine herbicides Y1 - 1997 ER - TY - JOUR A1 - Gajovic, Nenad A1 - Binyamin, Gary A1 - Warsinke, Axel A1 - Scheller, Frieder W. A1 - Heller, A. T1 - Operation of a miniature redox hydrogel-based pyruvate sensor in undiluted deoxygenated calf serum Y1 - 2000 ER - TY - JOUR A1 - Nitsche, Andreas A1 - Kurth, Andreas A1 - Dunkhorst, Anna A1 - Pänke, Oliver A1 - Sielaff, Hendrik A1 - Junge, Wolfgang A1 - Muth, Doreen A1 - Scheller, Frieder W. A1 - Stöcklein, Walter F. M. A1 - Pauli, Georg A1 - Kage, Andreas T1 - One-step selection of vaccinia virus binding DNA-aptamers by MonoLEX Y1 - 2007 UR - http://www.biomedcentral.com/1472-6750/7 U6 - https://doi.org/10.1186/1472-6750-7-48 ER -