TY - JOUR A1 - Puppe, Daniel A1 - Höhn, Axel A1 - Kaczorek, Danuta A1 - Wanner, Manfred A1 - Sommer, Michael T1 - As time goes by-Spatiotemporal changes of biogenic Si pools in initial soils of an artificial catchment in NE Germany JF - Applied soil ecology : a section of agriculture, ecosystems & environment KW - Biogenic silica KW - Diatom frustule KW - Testate amoeba shell KW - Sponge spicule KW - Initial ecosystem Y1 - 2016 U6 - https://doi.org/10.1016/j.apsoil.2016.01.020 SN - 0929-1393 SN - 1873-0272 VL - 105 SP - 9 EP - 16 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Zimmermann, Andreas T1 - Art. 116 Abs. 2 GG - ein verfassungsrechtliches Auslaufmodell JF - Die Öffentliche Verwaltung N2 - Zur Wiedergutmachung nationalsozialistischen Unrechts garantiert Art. 116 Abs. 2 GG Abkömmlingen von in diskriminierender Weise ausgebürgerten Deutschen die deutsche Staatsangehörigkeit. Dadurch soll der Zustand wiederhergestellt werden, der ohne die Ausbürgerung bestehen würde. Daher wird insoweit regelmäßig auf das geltende Staatsangehörigkeitsrecht abgestellt. § 4 Abs. 4 StAG hat im Jahr 2000 eine Beschränkung der Weitergabe der deutschen Staatsangehörigkeit für im Ausland geborene Kinder deutscher Staatsangehöriger eingeführt, die selbst bereits im Ausland geboren wurden. Dadurch wird möglicherweise der Anwendungsbereich des Art. 116 Abs. 2 GG dauerhaft signifikant eingeschränkt; unter Umständen wird die Norm gar obsolet. Vor diesem Hintergrund wird das Spannungsverhältnis zwischen der uneingeschränkten Anwendung des § 4 Abs. 4 StAG mit dem verfassungsrechtlich verbürgerten Recht auf Wiedereinbürgerung von Kindern zu Unrecht ausgebürgerter Deutscher erörtert. Y1 - 2016 SN - 0029-859X VL - 3 PB - Kohlhammer CY - Stuttgart ER - TY - JOUR A1 - Hanke-Gogokhia, Christin A1 - Wu, Zhijian A1 - Gerstner, Cecilia D. A1 - Frederick, Jeanne M. A1 - Zhang, Houbin A1 - Baehr, Wolfgang T1 - Arf-like Protein 3 (ARL3) Regulates Protein Trafficking and Ciliogenesis in Mouse Photoreceptors JF - The journal of biological chemistry N2 - Arf-like protein 3 (ARL3) is a ubiquitous small GTPase expressed in ciliated cells of plants and animals. Germline deletion of Arl3 in mice causes multiorgan ciliopathy reminiscent of Bardet-Biedl or Joubert syndromes. As photoreceptors are elegantly compartmentalized and have cilia, we probed the function of ARL3 (ADP-ribosylation factor (Arf)-like 3 protein) by generating rod photoreceptor-specific (prefix (rod)) and retina-specific (prefix (ret)) Arl3 deletions. In predegenerate (rod)Arl3(-/-) mice, lipidated phototransduction proteins showed trafficking deficiencies, consistent with the role of ARL3 as a cargo displacement factor for lipid-binding proteins. By contrast, (ret)Arl3(-/-) rods and cones expressing Cre recombinase during embryonic development formed neither connecting cilia nor outer segments and degenerated rapidly. Absence of cilia infers participation of ARL3 in ciliogenesis and axoneme formation. Ciliogenesis was rescued, and degeneration was reversed in part by subretinal injection of adeno-associated virus particles expressing ARL3-EGFP. The conditional knock-out phenotypes permitted identification of two ARL3 functions, both in the GTP-bound form as follows: one as a regulator of intraflagellar transport participating in photoreceptor ciliogenesis and the other as a cargo displacement factor transporting lipidated protein to the outer segment. Surprisingly, a farnesylated inositol polyphosphate phosphatase only trafficked from the endoplasmic reticulum to the Golgi, thereby excluding it from a role in photoreceptor cilia physiology. KW - animal model KW - gene knock-out KW - lipid-binding protein KW - photoreceptor KW - protein trafficking (Golgi) KW - retinal degeneration Y1 - 2016 U6 - https://doi.org/10.1074/jbc.M115.710954 SN - 0021-9258 SN - 1083-351X VL - 291 SP - 7142 EP - 7155 PB - American Society for Biochemistry and Molecular Biology CY - Bethesda ER - TY - JOUR A1 - Brzezinka, Krzysztof A1 - Altmann, Simone A1 - Czesnick, Hjördis A1 - Nicolas, Philippe A1 - Gorka, Michal A1 - Benke, Eileen A1 - Kabelitz, Tina A1 - Jähne, Felix A1 - Graf, Alexander A1 - Kappel, Christian A1 - Bäurle, Isabel T1 - Arabidopsis FORGETTER1 mediates stress-induced chromatin memory through nucleosome remodeling JF - eLife N2 - Plants as sessile organisms can adapt to environmental stress to mitigate its adverse effects. As part of such adaptation they maintain an active memory of heat stress for several days that promotes a more efficient response to recurring stress. We show that this heat stress memory requires the activity of the FORGETTER1 (FGT1) locus, with fgt1 mutants displaying reduced maintenance of heat-induced gene expression. FGT1 encodes the Arabidopsis thaliana orthologue of Strawberry notch (Sno), and the protein globally associates with the promoter regions of actively expressed genes in a heat-dependent fashion. FGT1 interacts with chromatin remodelers of the SWI/ SNF and ISWI families, which also display reduced heat stress memory. Genomic targets of the BRM remodeler overlap significantly with FGT1 targets. Accordingly, nucleosome dynamics at loci with altered maintenance of heat-induced expression are affected in fgt1. Together, our results suggest that by modulating nucleosome occupancy, FGT1 mediates stress-induced chromatin memory. Y1 - 2016 U6 - https://doi.org/10.7554/eLife.17061 SN - 2050-084X VL - 5 PB - eLife Sciences Publications CY - Cambridge ER - TY - JOUR A1 - Sun, Jing A1 - Cernoch, Peter A1 - Völkel, Antje A1 - Wei, Yuhan A1 - Ruokolainen, Janne A1 - Schlaad, Helmut T1 - Aqueous Self-Assembly of a Protein-Mimetic Ampholytic Block Copolypeptide JF - Macromolecules : a publication of the American Chemical Society N2 - This report describes the aggregation behavior of an ABC-type ampholytic block copolypeptide, poly(ethylene oxide)-block-poly(L-lysine)-block-poly(L-glutamate), in aqueous media in dependence of pH. Polypeptide secondary structures and self-assemblies are investigated by circular dichroism (CD), Fourier transform infrared (FT-IR) and NMR spectroscopy, zeta potential measurements, analytical ultracentrifugation (AUC), dynamic/static light scattering (DLS/SLS), and cryogenic transmission electron microscopy (cryoTEM). The polymer chains tend to form vesicles when the hydrophobic polypeptide helix is located at the chain end (acidic pH) and are existing as single chains when it is located in the center and flanked by the two hydrophilic segments (basic pH). Precipitation occurs in the intermediate pH range due to polyion complexation of the charged polypeptide segments. Y1 - 2016 U6 - https://doi.org/10.1021/acs.macromol.6b00817 SN - 0024-9297 SN - 1520-5835 VL - 49 SP - 5494 EP - 5501 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Gianniotis, Nikolaos A1 - Schnoerr, Christoph A1 - Molkenthin, Christian A1 - Bora, Sanjay Singh T1 - Approximate variational inference based on a finite sample of Gaussian latent variables JF - Pattern Analysis & Applications N2 - Variational methods are employed in situations where exact Bayesian inference becomes intractable due to the difficulty in performing certain integrals. Typically, variational methods postulate a tractable posterior and formulate a lower bound on the desired integral to be approximated, e.g. marginal likelihood. The lower bound is then optimised with respect to its free parameters, the so-called variational parameters. However, this is not always possible as for certain integrals it is very challenging (or tedious) to come up with a suitable lower bound. Here, we propose a simple scheme that overcomes some of the awkward cases where the usual variational treatment becomes difficult. The scheme relies on a rewriting of the lower bound on the model log-likelihood. We demonstrate the proposed scheme on a number of synthetic and real examples, as well as on a real geophysical model for which the standard variational approaches are inapplicable. KW - Bayesian inference KW - Posterior estimation KW - Expectation maximisation Y1 - 2016 U6 - https://doi.org/10.1007/s10044-015-0496-9 SN - 1433-7541 SN - 1433-755X VL - 19 SP - 475 EP - 485 PB - Springer CY - New York ER - TY - JOUR A1 - Grossi, Giuseppe A1 - Reichard, Christoph A1 - Ruggiero, Pasquale T1 - Appropriateness and Use of Performance Information in the Budgeting Process: Some Experiences from German and Italian Municipalities JF - Sports medicine N2 - This article contributes to the debate on the use of performance information in the context of public sector performance management. Based on case studies, the authors analyze the appropriateness of the performance information provided in the newly established performance budgets of municipalities in Germany and Italy. They also examine the interest of politicians and senior managers in using such information for decision-making and monitoring within the municipal budget cycle. They find that the use of performance information is generally quite modest, and that the interest of different local actors varies to a great extent. Politicians are generally less interested in such information than top managers, particularly chief financial officers. The results are discussed by applying a theoretical framework based on institutional and legitimacy theories, and are compared with the literature on performance information use. KW - Italy KW - Germany KW - local government KW - performance budget KW - performance information use KW - performance management Y1 - 2016 U6 - https://doi.org/10.1080/15309576.2015.1137770 SN - 1530-9576 SN - 1557-9271 VL - 39 SP - 581 EP - 606 PB - Springer CY - Abingdon ER - TY - JOUR A1 - Roggenbuck, Dirk A1 - Borghi, Maria Orietta A1 - Somma, Valentina A1 - Buettner, Thomas A1 - Schierack, Peter A1 - Hanack, Katja A1 - Grossi, Claudia A1 - Bodio, Caterina A1 - Macor, Paolo A1 - von Landenberg, Philipp A1 - Boccellato, Francesco A1 - Mahler, Michael A1 - Meroni, Pier Luigi T1 - Antiphospholipid antibodies detected by line immunoassay differentiate among patients with antiphospholipid syndrome, with infections and asymptomatic carriers JF - IEEE transactions on geoscience and remote sensing N2 - Background: Antiphospholipid antibodies (aPL) can be detected in asymptomatic carriers and infectious patients. The aim was to investigate whether a novel line immunoassay (LIA) differentiates between antiphospholipid syndrome (APS) and asymptomatic aPL+ carriers or patients with infectious diseases (infectious diseases controls (IDC)). Methods: Sixty-one patients with APS (56 primary, 22/56 with obstetric events only, and 5 secondary), 146 controls including 24 aPL+ asymptomatic carriers and 73 IDC were tested on a novel hydrophobic solid phase coated with cardiolipin (CL), phosphatic acid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, beta2-glycoprotein I (beta 2GPI), prothrombin, and annexin V. Samples were also tested by anti-CL and anti-beta 2GPI ELISAs and for lupus anticoagulant activity. Human monoclonal antibodies (humoAbs) against human beta 2GPI or PL alone were tested on the same LIA substrates in the absence or presence of human serum, purified human beta 2GPI or after CL-micelle absorption. Results: Comparison of LIA with the aPL-classification assays revealed good agreement for IgG/IgM a beta 2GPI and aCL. Anti-CL and anti-beta 2GPI IgG/IgM reactivity assessed by LIA was significantly higher in patients with APS versus healthy controls and IDCs, as detected by ELISA. IgG binding to CL and beta 2GPI in the LIA was significantly lower in aPL+ carriers and Venereal Disease Research Laboratory test (VDRL) + samples than in patients with APS. HumoAb against domain 1 recognized beta 2GPI bound to the LIA-matrix and in anionic phospholipid (PL) complexes. Absorption with CL micelles abolished the reactivity of a PL-specific humoAb but did not affect the binding of anti-beta 2GPI humoAbs. Conclusions: The LIA and ELISA have good agreement in detecting aPL in APS, but the LIA differentiates patients with APS from infectious patients and asymptomatic carriers, likely through the exposure of domain 1. KW - Antiphospholipid syndrome KW - Antiphospholipid antibody KW - Phospholipid binding proteins KW - Beta2-glycoprotein I KW - Line immunoassay Y1 - 2016 U6 - https://doi.org/10.1186/s13075-016-1018-x SN - 1478-6354 SN - 1478-6362 VL - 18 PB - BioMed Central CY - London ER - TY - JOUR A1 - Baraza, Lilechi D. A1 - Neser, Wekesa A1 - Jackson, Korir Cheruiyot A1 - Fredrick, Juma B. A1 - Dennis, Ochieno A1 - Wairimu, Kamau R. A1 - Keya, Aggrey Osogo A1 - Heydenreich, Matthias T1 - Antimicrobial Coumarins from the Oyster Culinary-Medicinal Mushroom, Pleurotus ostreatus (Agaricomycetes), from Kenya JF - International journal of medicinal mushrooms N2 - Pleurotus ostreatus has been widely used as food because of its nutritional and medicinal properties. These have been attributed to the presence of macronutrients, minerals, vitamins, and amino acids, among other secondary metabolites. There are, however, few reports on the antimicrobial activities of different classes of purified compounds from P. ostreatus. This led to the current study, the objective of which was to chemically characterize the antibiotic activities of P. ()streams against selected human pathogenic bacteria and endophytic fungi. Chemical structures were determined using spectroscopic methods and by comparison with values of related structures reported in the literature. Pure compounds from P. ostreatus were tested in vitro against pathogenic bacteria (Staphylococcus aureus and Escherichia coli) and endophytic fungi (Pencillium digitatum and Fusarium prolferatum). A new compound, (E)-5,7-dimethoxy-6-(3-methylbuta-1,3-dienyl)-2H-chromen-2-one (5-methoxy-(E)-suberodiene) (compound 2), along with ergosterol (compound I.) and 5,7-dimethoxy-6-(3-methylbut-2-enyl)-2H-chromen-2-one (toddaculin; compound 3), were isolated from the fruiting bodies of P. ostreatus. The growth of S. aureus,E proliferatum, and P. digitatum colonies was inhibited in media containing compound 2, with minimum inhibitory concentrations closely comparable to those of conventional antibiotics. KW - Escherichia coli KW - Fusarium proliferatum KW - medicinal mushrooms KW - Penicillium digitatum KW - Pleurotus ostreatus KW - Staphylococcus aureus Y1 - 2016 U6 - https://doi.org/10.1615/IntJMedMushrooms.v18.i10.60 SN - 1521-9437 SN - 1940-4344 VL - 18 SP - 905 EP - 913 PB - Begell House CY - Danbury ER - TY - JOUR A1 - Kunnus, Kristjan A1 - Josefsson, Ida A1 - Rajkovic, Ivan A1 - Schreck, Simon A1 - Quevedo, Wilson A1 - Beye, Martin A1 - Grübel, Sebastian A1 - Scholz, Mirko A1 - Nordlund, Dennis A1 - Zhang, Wenkai A1 - Hartsock, Robert W. A1 - Gaffney, Kelly J. A1 - Schlotter, William F. A1 - Turner, Joshua J. A1 - Kennedy, Brian A1 - Hennies, Franz A1 - Techert, Simone A1 - Wernet, Philippe A1 - Odelius, Michael A1 - Föhlisch, Alexander T1 - Anti-Stokes resonant x-ray Raman scattering for atom specific and excited state selective dynamics JF - NEW JOURNAL OF PHYSICS N2 - Ultrafast electronic and structural dynamics of matter govern rate and selectivity of chemical reactions, as well as phase transitions and efficient switching in functional materials. Since x-rays determine electronic and structural properties with elemental, chemical, orbital and magnetic selectivity, short pulse x-ray sources have become central enablers of ultrafast science. Despite of these strengths, ultrafast x-rays have been poor at picking up excited state moieties from the unexcited ones. With time-resolved anti-Stokes resonant x-ray Raman scattering (AS-RXRS) performed at the LCLS, and ab initio theory we establish background free excited state selectivity in addition to the elemental, chemical, orbital and magnetic selectivity of x-rays. This unparalleled selectivity extracts low concentration excited state species along the pathway of photo induced ligand exchange of Fe(CO)(5) in ethanol. Conceptually a full theoretical treatment of all accessible insights to excited state dynamics with AS-RXRS with transform-limited x-ray pulses is given-which will be covered experimentally by upcoming transform-limited x-ray sources. KW - ultrafast photochemistry KW - excited state selectivity KW - anti-Stokes resonant x-ray raman scattering KW - free electron lasers KW - resonant inelastic x-ray scattering Y1 - 2016 U6 - https://doi.org/10.1088/1367-2630/18/10/103011 SN - 1367-2630 VL - 18 PB - IOP Publ. Ltd. CY - Bristol ER -