TY  - JOUR
A1  - Choi, Youngeun
A1  - Kotthoff, Lisa
A1  - Olejko, Lydia
A1  - Resch-Genger, Ute
A1  - Bald, Ilko
T1  - DNA origami-based forster resonance energy-transfer Nanoarrays and their application as ratiometric sensors
JF  - ACS applied materials & interfaces
N2  - DNA origami nanostructures provide a platform where dye molecules can be arranged with nanoscale accuracy allowing to assemble multiple fluorophores without dye-dye aggregation. Aiming to develop a bright and sensitive ratiometric sensor system, we systematically studied the optical properties of nanoarrays of dyes built on DNA origami platforms using a DNA template that provides a high versatility of label choice at minimum cost. The dyes are arranged at distances, at which they efficiently interact by Forster resonance energy transfer (FRET). To optimize array brightness, the FRET efficiencies between the donor fluorescein (FAM) and the acceptor cyanine 3 were determined for different sizes of the array and for different arrangements of the dye molecules within the array. By utilizing nanoarrays providing optimum FRET efficiency and brightness, we subsequently designed a ratiometric pH nanosensor using coumarin 343 as a pH-inert FRET donor and FAM as a pH responsive acceptor. Our results indicate that the sensitivity of a ratiometric sensor can be improved simply by arranging the dyes into a well-defined array. The dyes used here can be easily replaced by other analyte-responsive dyes, demonstrating the huge potential of DNA nanotechnology for light harvesting, signal enhancement, and sensing schemes in life sciences.
KW  - DNA origami
KW  - nanoarray
KW  - FRET
KW  - ratiometric sensing
KW  - pH sensing
Y1  - 2018
U6  - https://doi.org/10.1021/acsami.8b03585
SN  - 1944-8244
SN  - 1944-8252
VL  - 10
IS  - 27
SP  - 23295
EP  - 23302
PB  - American Chemical Society
CY  - Washington
ER  -