TY - JOUR A1 - Müller, Christina Magdalena A1 - Huwe, Björn A1 - Wissemann, Volker A1 - Joshi, Jasmin Radha A1 - Gemeinholzer, Birgit T1 - Conservation genetic assessment of four plant species in a small replica of a steppe ecosystem >30 years after establishment JF - Biodiversity and conservation KW - Ex situ/in situ population genetic comparison KW - ISSR KW - Conservation genetics KW - Sand dune steppe and grassland vegetation KW - Botanic gardens Y1 - 2017 U6 - https://doi.org/10.1007/s10531-017-1381-1 SN - 0960-3115 SN - 1572-9710 VL - 26 SP - 2699 EP - 2716 PB - Springer CY - Dordrecht ER - TY - JOUR A1 - Dolgener, Nicola A1 - Schröder, Christiane A1 - Schneeweiss, N. A1 - Tiedemann, Ralph T1 - Genetic population structure of the Fire-bellied toad Bombina bombina in an area of high population density implications for conservation JF - Hydrobiologia : acta hydrobiologica, hydrographica, limnologica et protistologica N2 - In this study, we report the genetic population structure of the Fire-bellied toad Bombina bombina in Brandenburg (East Germany) in the context of conservation. We analysed 298 samples originating from 11 populations in Brandenburg using mitochondrial control region sequences and six polymorphic microsatellite loci. For comparison, we included one population each from Poland and Ukraine into our analysis. Within Brandenburg, we detected a moderate variability in the mitochondrial control region (19 different haplotypes) and at microsatellite loci (9-12 alleles per locus). These polymorphisms revealed a clear population structure among toads in Brandenburg, despite a relatively high overall population density and the moderate size of single populations (100-2000 individuals). The overall genetic population structure is consistent with a postglacial colonization from South East-Europe and a subsequent population expansion. Based on genetic connectivity, we infer Management Units (MUs) as targets for conservation. Our genetic survey identified MUs, within which human infrastructure is currently preventing any genetic exchange. We also detect an unintentional translocation from South East to North West Brandenburg, presumably in the course of fish stocking activities. Provided suitable conservation measures are taken, Brandenburg should continue to harbor large populations of this critically endangered species. KW - Conservation genetics KW - Fire-bellied toad KW - Fragmentation KW - Mitochondrial DNA KW - Microsatellites KW - Translocation Y1 - 2012 U6 - https://doi.org/10.1007/s10750-012-1016-1 SN - 0018-8158 VL - 689 IS - 1 SP - 111 EP - 120 PB - Springer CY - Dordrecht ER - TY - JOUR A1 - Sammler, Svenja A1 - Ketmaier, Valerio A1 - Havenstein, Katja A1 - Krause, Ulrike A1 - Curio, Eberhard A1 - Tiedemann, Ralph T1 - Mitochondrial control region I and microsatellite analyses of endangered Philippine hornbill species (Aves; Bucerotidae) detect gene flow between island populations and genetic diversity loss JF - BMC evolutionary biology N2 - Background: The Visayan Tarictic Hornbill (Penelopides panini) and the Walden's Hornbill (Aceros waldeni) are two threatened hornbill species endemic to the western islands of the Visayas that constitute - between Luzon and Mindanao - the central island group of the Philippine archipelago. In order to evaluate their genetic diversity and to support efforts towards their conservation, we analyzed genetic variation in similar to 600 base pairs (bp) of the mitochondrial control region I and at 12-19 nuclear microsatellite loci. The sampling covered extant populations, still occurring only on two islands (P. panini: Panay and Negros, A. waldeni: only Panay), and it was augmented with museum specimens of extinct populations from neighboring islands. For comparison, their less endangered (= more abundant) sister taxa, the Luzon Tarictic Hornbill (P. manillae) from the Luzon and Polillo Islands and the Writhed Hornbill (A. leucocephalus) from Mindanao Island, were also included in the study. We reconstructed the population history of the two Penelopides species and assessed the genetic population structure of the remaining wild populations in all four species. Results: Mitochondrial and nuclear data concordantly show a clear genetic separation according to the island of origin in both Penelopides species, but also unravel sporadic over-water movements between islands. We found evidence that deforestation in the last century influenced these migratory events. Both classes of markers and the comparison to museum specimens reveal a genetic diversity loss in both Visayan hornbill species, P. panini and A. waldeni, as compared to their more abundant relatives. This might have been caused by local extinction of genetically differentiated populations together with the dramatic decline in the abundance of the extant populations. Conclusions: We demonstrated a loss in genetic diversity of P. panini and A. waldeni as compared to their sister taxa P. manillae and A. leucocephalus. Because of the low potential for gene flow and population exchange across islands, saving of the remaining birds of almost extinct local populations - be it in the wild or in captivity - is particularly important to preserve the species' genetic potential. KW - Biogeography KW - Bucerotidae KW - Conservation genetics KW - Genetic diversity loss KW - Microsatellites KW - Mitochondrial control region I KW - Philippine archipelago KW - Phylogeography Y1 - 2012 U6 - https://doi.org/10.1186/1471-2148-12-203 SN - 1471-2148 VL - 12 IS - 25 PB - BioMed Central CY - London ER -