TY - THES
A1 - Pietzker, Christian
T1 - In-situ Wachstumsuntersuchungen beim reaktiven Anlassen von Cu, In Schichten in elementarem Schwefel
N2 - In dieser Arbeit wurde das reaktive Anlassen von dünnen Kupfer-Indium-Schichten in elementarem Schwefel mittels energiedispersiver Röntgenbeugung untersucht. Durch die simultane Aufnahme der Röntgenspektren und der Messung der diffusen Reflexion von Laserlicht der Wellenlänge 635 nm an der Oberfläche der Probe während des Schichtwachstums von CuInS2 konnte eine Methode zur Prozesskontrolle für ein Herstellungsverfahren von CuInS2 etabliert werden. Die Bildung von CuInS2 aus Kupfer-Indium-Vorläuferschichten wird dominiert von Umwandlungen der intermetallischen Phasen. CuInS2 wächst innerhalb der Aufheizperiode ab einer Temperatur von ca. 200°C aus der Phase Cu11In9. Jedoch zerfällt letztere metallische Phase in Cu16In9 und flüssiges Indium bei einer Temperatur von ca. 310°C. Das flüssige Indium reagiert im Falle von Kupferarmut mit dem Schwefel und führt zu einem zusätzlichen Reaktionspfad über InS zu CuIn5S8. Unter Präparationsbedingungen mit Kupferüberschuss wird das Indium in einer intermetallischen Phase gebunden.Erstmals konnte die Phase Digenite bei Temperaturen über 240°C beobachtet werden. Beim Abkühlen auf Raumtemperatur wandelt sich diese Phase unter dem Verbrauch von Schwefel in Covellite um.Für Proben mit Kupferüberschuss konnte eine Wachstumskinetik proportional zur Temperatur beobachtet werden. Dieses Verhalten wurde durch eine stress-induzierte Diffusion als dominierenden Reaktionsmechanismus interpretiert. Dabei werden während der Bildung von CuInS2 durch unterschiedliche Ausdehnungen der metallischen und sulfidischen Schichten eine Spannung in der CuInS2-Schicht induziert, die nach Überschreiten einer Grenzspannung zu Rissen in der CuInS2-Schicht führt. Entlang dieser Risse findet ein schneller Transport der Metalle zur Oberfläche, wo diese mit dem Schwefel reagieren können, statt. Die Risse heilen durch die Bildung neuen Sulfids wieder aus.
N2 - In this work the reactive annealing of thin copper and indium films in elemental sulphur was investigated by energy dispersive X-ray diffraction. Measuring simultanously laser light diffusively reflected from the growth surface, a simple method for process monitoring could be established. The process monitoring using 635 nm laser light can now independently be used in production.The growth of CuInS2 from copper-indium precursors is dominated by transitions between intermetallic phases. CuInS2 growths in the heat up period above 200 °C from the phase Cu11In9. However the latter metallic phase decomposes into Cu16In9 and liquid indium at a temperature of 310 °C. The liquid indium reacts in the case of copper deficiency with sulphur to InS. This leads to an additional reaction path via InS to CuIn5S8. Under preparation conditions with copper excess to the contrary, indium is bound in an intermetallic phase.For the first time the phase Digenite could be observed in a growth experiment at temperatures above 240 °C. During cool down to room temperature this phase transforms to Covellite by consumption of sulphur.For samples with copper excess a growth kinetic proportional to the temperature was observed. This behaviour is interpreted by a stress induced reaction mechanism: During the formation of CuInS2, strain in the CuInS2 thin film is induced due to different expansion coefficients of the metallic and sulphurous phases. After transgression of a certain strain limit, cracks within CuInS2 are formed. Along these cracks, fast transport of metallic species to the surface can occur. There these species can react with the sulphur. The cracks can heal up by the formation of new sulphides.
KW - CuInS2
KW - Chalkopyrit
KW - Wachstum
KW - EDXRD
KW - LLS
KW - elementarer Schwefel
KW - reaktives Anlassen
KW - CuInS2
KW - Chalcopyrite
KW - growth
KW - EDXRD
KW - LLS
KW - elemental sulphur
KW - reactive annealing
Y1 - 2003
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-0001219
ER -
TY - THES
A1 - Geißler, Katja
T1 - Lebensstrategien seltener Stromtalpflanzen : autökologische Untersuchung von Cnidium dubium, Gratiola officinalis und Juncus atratus unter besonderer Berücksichtigung ihrer Stressresistenz
T1 - Life strategies of rare river corridor plants : autecological investigation of Cnidium dubium, Gratiola officinalis and Juncus atratus with special consideration of their stress resistance
N2 - Die vorliegende Dissertation behandelt die Ökologie von Cnidium dubium (Schkuhr) Thell. (Sumpf-Brenndolde), Gratiola officinalis L. (Gottes-Gnadenkraut) und Juncus atratus Krocker (Schwarze Binse), drei gefährdeten Arten, die als sogenannte Stromtalpflanzen in Mitteleuropa in ihrem Vorkommen eng an die Flussauen gebunden sind. Die Arbeit basiert auf verschiedenen Simulationsexperimenten und Feldstudien in der Unteren Havelniederung, einem „Feuchtgebiet von internationaler Bedeutung“. Sie behandelt Themenkomplexe wie das Samenbankverhalten, die Samenkeimung, die Stickstofflimitierung, die Konkurrenzkraft, das Verhalten der Pflanzen nach einer Sommertrockenheit und nach einer Winter/Frühjahrsüberflutung. Ferner widmet sie sich der Populationsbiologie der Arten und dem Verhalten der Pflanzen nach besonderen Störungsereignissen wie Mahd, Herbivorie und der Sommerflut 2002. Der Leser erfährt, wie die Pflanzen in verschiedenen Lebensphasen auf die auentypische Umwelt reagieren und erhält umfassende Einblicke in physiologische Mechanismen, die der Anpassung an die typischen Bedingungen einer mitteleuropäischen Flussaue dienen. Eine Interpretation der Ergebnisse zeigt auf, welche der spezifischen Eigenschaften zur Gefährdung der drei Stromtalarten beitragen. Die Arbeit ist für den Arten-, Biotop- und Landschaftsschutz interessant. Darüber hinaus bietet sie zahlreiche Anknüpfungspunkte zur ökophysiologischen Grundlagenforschung. Die verstärkte Nutzung physiologischer Methoden bei der Klärung ökologischer Fragestellungen wird angeregt.
N2 - The thesis deals with the ecology of three endangered European river corridor angiosperms Cnidium dubium (Schkuhr) Thell., Gratiola officinalis L. und Juncus atratus Krocker. The study is based on different experimental approaches and field surveys in a wetland along the Lower Havel River, a designated German Ramsar-site (Wetland of International Importance). This involves the examination of aspects of seed bank dynamics, germination, nitrogen limitation, competitive ability, and the response of plants to summer drought and/or winter/spring flooding. The thesis continues with a detailed study of the population biology of the species at natural sites and the response of these plants to specific disturbances like mowing, herbivory and the severe summer flooding in 2002. The reader learns about the traits of the three plant species to tolerate the typical conditions their natural sites are exposed to in different phases of their life cycle. He gets a comprehensive look at physiological means by which plants can adapt to the prevailing conditions of European river lowlands. The interpretation of the results is used to reveal specific plant traits, which may contribute to the endangerment of the three river corridor plants. As such, this thesis is interesting for protection of species, biotopes and landscapes. Furthermore, it provides numerous close connections to fundamental research from an ecophysiological perspective. The increased use of physiological methods is recommended in order to be able to adequately resolve ecological problems.
KW - untere Havelniederung
KW - seltene Pflanzen
KW - Stoffwechsel
KW - Wachstum
KW - Samen
KW - Hypoxie
KW - Trockenstress
KW - Konkurrenz
KW - Mahd
KW - lower Havel river wetland
KW - rare plants
KW - metabolism
KW - growth
KW - seeds
KW - hypoxia
KW - drought stress
KW - competition
KW - mowing
Y1 - 2008
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-17468
ER -
TY - JOUR
A1 - Arvidsson, Samuel Janne
A1 - Perez-Rodriguez, Paulino
A1 - Müller-Röber, Bernd
T1 - A growth phenotyping pipeline for Arabidopsis thaliana integrating image analysis and rosette area modeling for robust quantification of genotype effects
JF - New phytologist : international journal of plant science
N2 - To gain a deeper understanding of the mechanisms behind biomass accumulation, it is important to study plant growth behavior. Manually phenotyping large sets of plants requires important human resources and expertise and is typically not feasible for detection of weak growth phenotypes. Here, we established an automated growth phenotyping pipeline for Arabidopsis thaliana to aid researchers in comparing growth behaviors of different genotypes.
The analysis pipeline includes automated image analysis of two-dimensional digital plant images and evaluation of manually annotated information of growth stages. It employs linear mixed-effects models to quantify genotype effects on total rosette area and relative leaf growth rate (RLGR) and ANOVAs to quantify effects on developmental times.
Using the system, a single researcher can phenotype up to 7000 plants d(-1). Technical variance is very low (typically < 2%). We show quantitative results for the growth-impaired starch-excessmutant sex4-3 and the growth-enhancedmutant grf9.
We show that recordings of environmental and developmental variables reduce noise levels in the phenotyping datasets significantly and that careful examination of predictor variables (such as d after sowing or germination) is crucial to avoid exaggerations of recorded phenotypes and thus biased conclusions.
KW - development
KW - growth
KW - leaf area
KW - modeling
KW - phenotyping
Y1 - 2011
U6 - https://doi.org/10.1111/j.1469-8137.2011.03756.x
SN - 0028-646X
VL - 191
IS - 3
SP - 895
EP - 907
PB - Wiley-Blackwell
CY - Malden
ER -
TY - GEN
A1 - Lenhard, Michael
T1 - All's well that ends well
BT - arresting cell proliferation in leaves
T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2 - The transition from cell proliferation to cell expansion is critical for determining leaf size. Andriankaja et al. (2012) demonstrate that in leaves of dicotyledonous plants, a basal proliferation zone is maintained for several days before abruptly disappearing, and that chloroplast differentiation is required to trigger the onset of cell expansion.
T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 906
KW - arabidopsis-thaliana
KW - genetic-control
KW - growth
KW - size
KW - curvature
Y1 - 2020
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-438035
SN - 1866-8372
IS - 906
SP - 9
EP - 11
ER -
TY - JOUR
A1 - Ribeiro, Dimas M.
A1 - Araujo, Wagner L.
A1 - Fernie, Alisdair R.
A1 - Schippers, Jos H. M.
A1 - Müller-Röber, Bernd
T1 - Translatome and metabolome effects triggered by gibberellins during rosette growth in Arabidopsis
JF - Journal of experimental botany
N2 - Although gibberellins (GAs) are well known for their growth control function, little is known about their effects on primary metabolism. Here the modulation of gene expression and metabolic adjustment in response to changes in plant (Arabidopsis thaliana) growth imposed on varying the gibberellin regime were evaluated. Polysomal mRNA populations were profiled following treatment of plants with paclobutrazol (PAC), an inhibitor of GA biosynthesis, and gibberellic acid (GA(3)) to monitor translational regulation of mRNAs globally. Gibberellin levels did not affect levels of carbohydrates in plants treated with PAC and/or GA(3). However, the tricarboxylic acid cycle intermediates malate and fumarate, two alternative carbon storage molecules, accumulated upon PAC treatment. Moreover, an increase in nitrate and in the levels of the amino acids was observed in plants grown under a low GA regime. Only minor changes in amino acid levels were detected in plants treated with GA(3) alone, or PAC plus GA(3). Comparison of the molecular changes at the transcript and metabolite levels demonstrated that a low GA level mainly affects growth by uncoupling growth from carbon availability. These observations, together with the translatome changes, reveal an interaction between energy metabolism and GA-mediated control of growth to coordinate cell wall extension, secondary metabolism, and lipid metabolism.
KW - Gibberellin
KW - growth
KW - paclobutrazol
KW - primary metabolism
KW - translatome
Y1 - 2012
U6 - https://doi.org/10.1093/jxb/err463
SN - 0022-0957
VL - 63
IS - 7
SP - 2769
EP - 2786
PB - Oxford Univ. Press
CY - Oxford
ER -
TY - JOUR
A1 - Lisso, Janina
A1 - Schröder, Florian
A1 - Müssig, Carsten
T1 - EXO modifies sucrose and trehalose responses and connects the extracellular carbon status to growth
JF - Frontiers in plant science
N2 - Plants have the capacity to adapt growth to changing environmental conditions. This implies the modulation of metabolism according to the availability of carbon (C). Particular interest in the response to the C availability is based on the increasing atmospheric levels of CO2. Several regulatory pathways that link the C status to growth have emerged. The extracellular EXO protein is essential for cell expansion and promotes shoot and root growth. Homologous proteins were identified in evolutionarily distant green plants. We show here that the EXO protein connects growth with C responses. The exo mutant displayed altered responses to exogenous sucrose supplemented to the growth medium. Impaired growth of the mutant in synthetic medium was associated with the accumulation of starch and anthocyanins, altered expression of sugar-responsive genes, and increased abscisic acid levels. Thus, EXO modulates several responses related to the C availability. Growth retardation on medium supplemented with 2-deoxy-glucose, mannose, and palatinose was similar to the wildtype. Trehalose feeding stimulated root growth and shoot biomass production of exoplants where as it inhibited growth of the wildtype. The phenotypic features of the exo mutant suggest that apoplastic processes coordinate growth and C responses.
KW - EXO
KW - growth
KW - sugar response
KW - trehalose
KW - apoplast
Y1 - 2013
U6 - https://doi.org/10.3389/fpls.2013.00219
SN - 1664-462X
VL - 4
IS - 25
PB - Frontiers Research Foundation
CY - Lausanne
ER -
TY - JOUR
A1 - Petrov, Veselin
A1 - Schippers, Jos
A1 - Benina, Maria
A1 - Minkov, Ivan
A1 - Müller-Röber, Bernd
A1 - Gechev, Tsanko S.
T1 - In search for new players of the oxidative stress network by phenotyping an Arabidopsis T-DNA mutant collection on reactive oxygen species-eliciting chemicals
JF - Plant omics
N2 - The ability of some chemical compounds to cause oxidative stress offers a fast and convenient way to study the responses of plants to reactive oxygen species (ROS). In order to unveil potential novel genetic players of the ROS-regulatory network, a population of similar to 2,000 randomly selected Arabidopsis thaliana T-DNA insertion mutants was screened for ROS sensitivity/resistance by growing seedlings on agar medium supplemented with stress-inducing concentrations of the superoxide-eliciting herbicide methyl viologen or the catalase inhibitor 3-amino-triazole. A semi-robotic setup was used to capture and analyze images of the chemically treated seedlings which helped interpret the screening results by providing quantitative information on seedling area and healthy-to-chlorotic tissue ratios for data verification. A ROS-related phenotype was confirmed in three of the initially selected 33 mutant candidates, which carry T-DNA insertions in genes encoding a Ring/Ubox superfamily protein, ABI5 binding protein 1 (AFP1), previously reported to be involved in ABA signaling, and a protein of unknown function, respectively. In addition, we identified six mutants, most of which have not been described yet, that are related to growth or chloroplast development and show defects in a ROS-independent manner. Thus, semi-automated image capturing and phenotyping applied on publically available T-DNA insertion collections adds a simple means for discovering novel mutants in complex physiological processes and identifying the genes involved.
KW - growth
KW - image analysis
KW - methyl viologen
KW - LemnaTec
KW - screening
KW - superoxide
Y1 - 2013
SN - 1836-0661
VL - 6
IS - 1
SP - 46
EP - 54
PB - Southern Cross Publ.
CY - Lismore
ER -
TY - GEN
A1 - Hermanussen, Michael
A1 - Scheffler, Christiane
A1 - Groth, Detlef
A1 - Aßmann, Christian
T1 - Height and skeletal morphology in relation to modern life style
T2 - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe
N2 - Height and skeletal morphology strongly relate to life style. Parallel to the decrease in physical activity and locomotion, modern people are slimmer in skeletal proportions. In German children and adolescents, elbow breadth and particularly relative pelvic breadth (50th centile of bicristal distance divided by body height) have significantly decreased in recent years. Even more evident than the changes in pelvic morphology are the rapid changes in body height in most modern countries since the end-19th and particularly since the mid-20th century. Modern Japanese mature earlier; the age at take-off (ATO, the age at which the adolescent growth spurt starts) decreases, and they are taller at all ages. Preece-Baines modelling of six national samples of Japanese children and adolescents, surveyed between 1955 and 2000, shows that this gain in height is largely an adolescent trend, whereas height at take-off (HTO) increased by less than 3 cm since 1955; adolescent growth (height gain between ATO and adult age) increased by 6 cm. The effect of globalization on the modern post-war Japanese society ("community effect in height") on adolescent growth is discussed.
T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 869
KW - pelvic breadth
KW - elbow breadth
KW - growth
KW - adult height
KW - community effect in height
Y1 - 2020
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-434814
SN - 1866-8372
IS - 869
ER -
TY - GEN
A1 - Johnson, Kim L.
A1 - Ramm, Sascha
A1 - Kappel, Christian
A1 - Ward, Sally
A1 - Leyser, Ottoline
A1 - Sakamoto, Tomoaki
A1 - Kurata, Tetsuya
A1 - Bevan, Michael W.
A1 - Lenhard, Michael
T1 - The tinkerbell (tink) mutation identifies the dual-specificity MAPK phosphatase INDOLE- 3-BUTYRIC ACID-RESPONSE5 (IBR5) as a novel regulator of organ size in Arabidopsis
T2 - PLoS ONE
N2 - Mitogen-activated dual-specificity MAPK phosphatases are important negative regulators in the MAPK signalling pathways responsible for many essential processes in plants. In a screen for mutants with reduced organ size we have identified a mutation in the active site of the dual-specificity MAPK phosphatase INDOLE-3-BUTYRIC ACID-RESPONSE5 (IBR5) that we named tinkerbell (tink) due to its small size. Analysis of the tink mutant indicates that IBR5 acts as a novel regulator of organ size that changes the rate of growth in petals and leaves. Organ size and shape regulation by IBR5 acts independently of the KLU growth-regulatory pathway. Microarray analysis of tink/ibr5-6 mutants identified a likely role for this phosphatase in male gametophyte development. We show that IBR5 may influence the size and shape of petals through auxin and TCP growth regulatory pathways.
T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 427
KW - class-i
KW - protein phosphatase
KW - auxin
KW - responses
KW - thaliana
KW - kinase
KW - growth
KW - interacts
KW - distinct
KW - pathway
Y1 - 2018
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-410245
ER -
TY - JOUR
A1 - Bemer, Marian
A1 - van Mourik, Hilda
A1 - Muino, Jose M.
A1 - Ferrandiz, Cristina
A1 - Kaufmann, Kerstin
A1 - Angenent, Gerco C.
T1 - FRUITFULL controls SAUR10 expression and regulates Arabidopsis growth and architecture
JF - Journal of experimental botany
N2 - MADS-domain transcription factors are well known for their roles in plant development and regulate sets of downstream genes that have been uncovered by high-throughput analyses. A considerable number of these targets are predicted to function in hormone responses or responses to environmental stimuli, suggesting that there is a close link between developmental and environmental regulators of plant growth and development. Here, we show that the Arabidopsis MADS-domain factor FRUITFULL (FUL) executes several functions in addition to its noted role in fruit development. Among the direct targets of FUL, we identified SMALL AUXIN UPREGULATED RNA 10 (SAUR10), a growth regulator that is highly induced by a combination of auxin and brassinosteroids and in response to reduced R:FR light. Interestingly, we discovered that SAUR10 is repressed by FUL in stems and inflorescence branches. SAUR10 is specifically expressed at the abaxial side of these branches and this localized activity is influenced by hormones, light conditions and by FUL, which has an effect on branch angle. Furthermore, we identified a number of other genes involved in hormone pathways and light signalling as direct targets of FUL in the stem, demonstrating a connection between developmentally and environmentally regulated growth programs.
KW - Architecture
KW - auxin
KW - branching
KW - FRUITFULL
KW - growth
KW - hormones
KW - light response
KW - MADS-box transcription factor
KW - SAUR
Y1 - 2017
U6 - https://doi.org/10.1093/jxb/erx184
SN - 0022-0957
SN - 1460-2431
VL - 68
SP - 3391
EP - 3403
PB - Oxford Univ. Press
CY - Oxford
ER -
TY - JOUR
A1 - Shahnejat-Bushehri, Sara
A1 - Allu, Annapurna Devi
A1 - Mehterov, Nikolay
A1 - Thirumalaikumar, Venkatesh P.
A1 - Alseekh, Saleh
A1 - Fernie, Alisdair R.
A1 - Mueller-Roeber, Bernd
A1 - Balazadeh, Salma
T1 - Arabidopsis NAC Transcription Factor JUNGBRUNNEN1 Exerts Conserved Control Over Gibberellin and Brassinosteroid Metabolism and Signaling Genes in Tomato
JF - Frontiers in plant science
N2 - The Arabidopsis thaliana NAC transcription factor JUNGBRUNNEN1 (AtJUB1) regulates growth by directly repressing GA3ox1 and DWF4, two key genes involved in gibberellin (GA) and brassinosteroid (BR) biosynthesis, respectively, leading to GA and BR deficiency phenotypes. AtJUB1 also reduces the expression of PIF4, a bHLH transcription factor that positively controls cell elongation, while it stimulates the expression of DELLA genes, which are important repressors of growth. Here, we extend our previous findings by demonstrating that AtJUB1 induces similar GA and BR deficiency phenotypes and changes in gene expression when overexpressed in tomato (Solanum lycopersicum). Importantly, and in accordance with the growth phenotypes observed, AtJUB1 inhibits the expression of growth-supporting genes, namely the tomato orthologs of GA3ox1, DWF4 and PIF4, but activates the expression of DELLA orthologs, by directly binding to their promoters. Overexpression of AtJUB1 in tomato delays fruit ripening, which is accompanied by reduced expression of several ripeningrelated genes, and leads to an increase in the levels of various amino acids (mostly proline, beta-alanine, and phenylalanine), gamma-aminobutyric acid (GABA), and major organic acids including glutamic acid and aspartic acid. The fact that AtJUB1 exerts an inhibitory effect on the GA/BR biosynthesis and PIF4 genes but acts as a direct activator of DELLA genes in both, Arabidopsis and tomato, strongly supports the model that the molecular constituents of the JUNGBRUNNEN1 growth control module are considerably conserved across species.
KW - Arabidopsis
KW - tomato
KW - fruit
KW - growth
KW - transcription factor
KW - gibberellic acid
KW - brassinosteroid
KW - DELLA proteins
Y1 - 2017
U6 - https://doi.org/10.3389/fpls.2017.00214
SN - 1664-462X
VL - 8
PB - Frontiers Research Foundation
CY - Lausanne
ER -
TY - GEN
A1 - Hornick, Thomas
A1 - Bach, Lennart T.
A1 - Crawfurd, Katharine J.
A1 - Spilling, Kristian
A1 - Achterberg, Eric Pieter
A1 - Woodhouse, Jason Nicholas
A1 - Schulz, Kai Georg
A1 - Brussaard, Corina P. D.
A1 - Riebesell, Ulf
A1 - Grossart, Hans-Peter
T1 - Ocean acidification impacts bacteria–phytoplankton coupling at low-nutrient conditions
T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2 - The oceans absorb about a quarter of the annually produced anthropogenic atmospheric carbon dioxide (CO2), resulting in a decrease in surface water pH, a process termed ocean acidification (OA). Surprisingly little is known about how OA affects the physiology of heterotrophic bacteria or the coupling of heterotrophic bacteria to phytoplankton when nutrients are limited. Previous experiments were, for the most part, undertaken during productive phases or following nutrient additions designed to stimulate algal blooms. Therefore, we performed an in situ large-volume mesocosm (similar to 55 m(3)) experiment in the Baltic Sea by simulating different fugacities of CO2 (fCO(2)) extending from present to future conditions. The study was conducted in July-August after the nominal spring bloom, in order to maintain low-nutrient conditions throughout the experiment. This resulted in phytoplankton communities dominated by small-sized functional groups (picophytoplankton). There was no consistent fCO(2)-induced effect on bacterial protein production (BPP), cell-specific BPP (csBPP) or biovolumes (BVs) of either free-living (FL) or particle-associated (PA) heterotrophic bacteria, when considered as individual components (univariate analyses). Permutational Multivariate Analysis of Variance (PERMANOVA) revealed a significant effect of the fCO(2) treatment on entire assemblages of dissolved and particulate nutrients, metabolic parameters and the bacteria-phytoplankton community. However, distance-based linear modelling only identified fCO(2) as a factor explaining the variability observed amongst the microbial community composition, but not for explaining variability within the metabolic parameters. This suggests that fCO(2) impacts on microbial metabolic parameters occurred indirectly through varying physicochemical parameters and microbial species composition. Cluster analyses examining the co-occurrence of different functional groups of bacteria and phytoplankton further revealed a separation of the four fCO(2)-treated mesocosms from both control mesocosms, indicating that complex trophic interactions might be altered in a future acidified ocean. Possible consequences for nutrient cycling and carbon export are still largely unknown, in particular in a nutrient-limited ocean.
T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 667
KW - northern Baltic Sea
KW - inorganic nutrients
KW - mesocosm experiment
KW - elevated CO2
KW - heterotrophic bacteria
KW - organic-carbon
KW - bacterioplankton
KW - seawater
KW - growth
KW - temperature
Y1 - 2019
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-417126
SN - 1866-8372
IS - 667
ER -
TY - GEN
A1 - Zemella, Anne
A1 - Thoring, Lena
A1 - Hoffmeister, Christian
A1 - Šamalíková, Mária
A1 - Ehren, Patricia
A1 - Wüstenhagen, Doreen Anja
A1 - Kubick, Stefan
T1 - Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin
T2 - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe
N2 - As one of the most complex post-translational modification, glycosylation is widely involved in cell adhesion, cell proliferation and immune response. Nevertheless glycoproteins with an identical polypeptide backbone mostly differ in their glycosylation patterns. Due to this heterogeneity, the mapping of different glycosylation patterns to their associated function is nearly impossible. In the last years, glycoengineering tools including cell line engineering, chemoenzymatic remodeling and site-specific glycosylation have attracted increasing interest. The therapeutic hormone erythropoietin (EPO) has been investigated in particular by various groups to establish a production process resulting in a defined glycosylation pattern. However commercially available recombinant human EPO shows batch-to-batch variations in its glycoforms. Therefore we present an alternative method for the synthesis of active glycosylated EPO with an engineered O-glycosylation site by combining eukaryotic cell-free protein synthesis and site-directed incorporation of non-canonical amino acids with subsequent chemoselective modifications.
T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 824
KW - recombinat-human-erythropoietin
KW - glycosylation
KW - expression
KW - site
KW - anemia
KW - CDNA
KW - glycoprotein
KW - purification
KW - cloning
KW - growth
Y1 - 2020
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-427017
IS - 824
ER -
TY - GEN
A1 - Igual Gil, Carla
A1 - Ost, Mario
A1 - Kasch, Juliane
A1 - Schumann, Sara
A1 - Heider, Sarah
A1 - Klaus, Susanne
T1 - Role of GDF15 in active lifestyle induced metabolic adaptations and acute exercise response in mice
T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2 - Physical activity is an important contributor to muscle adaptation and metabolic health. Growth differentiation factor 15 (GDF15) is established as cellular and nutritional stress-induced cytokine but its physiological role in response to active lifestyle or acute exercise is unknown. Here, we investigated the metabolic phenotype and circulating GDF15 levels in lean and obese male C57BI/6J mice with long-term voluntary wheel running (VWR) intervention. Additionally, treadmill running capacity and exercise-induced muscle gene expression was examined in GDF15-ablated mice. Active lifestyle mimic via VWR improved treadmill running performance and, in obese mice, also metabolic phenotype. The post-exercise induction of skeletal muscle transcriptional stress markers was reduced by VWR. Skeletal muscle GDF15 gene expression was very low and only transiently increased post-exercise in sedentary but not in active mice. Plasma GDF15 levels were only marginally affected by chronic or acute exercise. In obese mice, VWR reduced GDF15 gene expression in different tissues but did not reverse elevated plasma GDF15. Genetic ablation of GDF15 had no effect on exercise performance but augmented the post exercise expression of transcriptional exercise stress markers (Atf3, Atf6, and Xbp1s) in skeletal muscle. We conclude that skeletal muscle does not contribute to circulating GDF15 in mice, but muscle GDF15 might play a protective role in the exercise stress response.
T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1090
KW - skeletal-muscle
KW - growth
KW - induction
KW - insulin
KW - activation
KW - increases
KW - glucagon
KW - health
KW - gene
KW - diet
Y1 - 2021
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-460541
SN - 1866-8372
IS - 1090
ER -
TY - JOUR
A1 - Olas, Justyna Jadwiga
A1 - Fichtner, Franziska
A1 - Apelt, Federico
T1 - All roads lead to growth
BT - imaging-based and biochemical methods to measure plant growth
JF - Journal of experimental botany
N2 - Plant growth is a highly complex biological process that involves innumerable interconnected biochemical and signalling pathways. Many different techniques have been developed to measure growth, unravel the various processes that contribute to plant growth, and understand how a complex interaction between genotype and environment determines the growth phenotype. Despite this complexity, the term 'growth' is often simplified by researchers; depending on the method used for quantification, growth is viewed as an increase in plant or organ size, a change in cell architecture, or an increase in structural biomass. In this review, we summarise the cellular and molecular mechanisms underlying plant growth, highlight state-of-the-art imaging and non-imaging-based techniques to quantitatively measure growth, including a discussion of their advantages and drawbacks, and suggest a terminology for growth rates depending on the type of technique used.
KW - biomass
KW - growth
KW - imaging
KW - kinematics
KW - morphometrics
KW - phenomics
KW - phenotyping
KW - relative expansion rate of growth (RER)
KW - relative growth
KW - rate (RGR)
Y1 - 2019
U6 - https://doi.org/10.1093/jxb/erz406
SN - 0022-0957
SN - 1460-2431
VL - 71
IS - 1
SP - 11
EP - 21
PB - Oxford Univ. Press
CY - Oxford
ER -
TY - JOUR
A1 - Rogol, Alan D.
ED - Scheffler, Christiane
ED - Koziel, Slawomir
ED - Hermanussen, Michael
ED - Bogin, Barry
T1 - Settings Perspective
BT - Bridging the Gap between Human Biology and Public Health
T2 - Human Biology and Public Health
KW - growth
KW - maturation
KW - populations
KW - secular change
Y1 - 2021
U6 - https://doi.org/10.52905/hbph.v1.2
SN - 2748-9957
VL - 2021
IS - 1
SP - 1
EP - 2
PB - Universitätsverlag Potsdam
CY - Potsdam
ER -
TY - JOUR
A1 - Hermanussen, Michael
A1 - Erofeev, Sergei
A1 - Scheffler, Christiane
T1 - The socio-endocrine regulation of human growth
JF - Acta paediatrica : nurturing the child
N2 - Aim Growth is a multifarious phenomenon that has been studied by nutritionists, economists, paediatric endocrinologists; archaeologists, child psychologists and other experts. Yet, a unifying theory of understanding growth regulation is still lacking. Method Critical review of the literature. Results We summarise evidence linking social competition and its effect on hierarchies in social structures, with the neuronal networks of the ventromedial hypothalamus and body size. The endocrine signalling system regulating growth hormone, Insulin-like-Growth-Factor1 and skeletal growth, is well conserved in the evolution of vertebrata for some 400 million years. The link between size and status permits adaptive plasticity, competitive growth and strategic growth adjustments also in humans. Humans perceive size as a signal of dominance with tallness being favoured and particularly prevalent in the upper social classes. Conclusion Westernised societies are competitive. People are tall, and "open to change." Social values include striving for status and prestige implying socio-economic domination. We consider the transition of political and social values following revolutions and civil wars, as key elements that interact with the evolutionarily conserved neuroendocrine competence for adaptive developmental plasticity, overstimulate the hypothalamic growth regulation and finally lead to the recent historic increases in average height.
KW - competitive growth
KW - developmental plasticity
KW - evolution
KW - growth
KW - hormone-releasing hormone
KW - strategic growth adjustments
Y1 - 2022
U6 - https://doi.org/10.1111/apa.16504
SN - 0803-5253
SN - 1651-2227
PB - Wiley
CY - Hoboken
ER -