TY  - JOUR
A1  - Rosencrantz, Sophia
A1  - Tang, Jo Sing Julia
A1  - Schulte-Osseili, Christine
A1  - Böker, Alexander
A1  - Rosencrantz, Ruben R.
T1  - Glycopolymers by RAFT Polymerization as Functional Surfaces for Galectin-3
JF  - Macromolecular chemistry and physics
N2  - Glycan-protein interactions are essential biological processes with many disease-related modulations and variations. One of the key proteins involved in tumor progression and metastasis is galectin-3 (Gal-3). A lot of effort is put into the development of Gal-3 inhibitors as new therapeutic agents. The avidity of glycan-protein interactions is strongly enhanced by multivalent ligand presentation. Multivalent presentation of glycans can be accomplished by utilizing glycopolymers, which are polymers with pendent glycan groups. For the production of glycopolymers, glycomonomers are synthesized by a regioselective, microwave-assisted approach starting from lactose. The resulting methacrylamide derivatives are polymerized by RAFT and immobilized on gold surfaces using the trithiocarbonate group of the chain transfer agent. Surface plasmon resonance spectroscopy enables the label free kinetic characterization of Gal-3 binding to these multivalent glycopolymers. The measurements indicate oligomerization of Gal-3 upon exposure to multivalent environments and reveal strong specific interaction with the immobilized polymers.
KW  - galectin-3
KW  - glycopolymers
KW  - multivalency
KW  - RAFT
KW  - surface plasmon resonance
Y1  - 2019
U6  - https://doi.org/10.1002/macp.201900293
SN  - 1022-1352
SN  - 1521-3935
VL  - 220
IS  - 20
PB  - Wiley-VCH
CY  - Weinheim
ER  -