TY  - JOUR
A1  - Techen, Anne
A1  - Hille, Carsten
A1  - Dosche, Carsten
A1  - Kumke, Michael Uwe
T1  - Fluorescence study of drug-carrier interactions in CTAB/PBS buffer model systems
JF  - Journal of colloid and interface science
N2  - The well-known cationic surfactant hexadecyltrimethylammonium bromide (CTAB) was used as a model carrier to study drug-carrier interactions with fluorescence probes (5-hexadecanoylaminofluorescein (HAF) and 2,10-bis-(3-aminopropyloxy)dibenzo[aj]perylene-8,16-dione (NIR 628) by applying ensemble as well as single molecule fluorescence techniques. The impact of the probes on the micelle parameters (critical micelle concentration, average aggregation number, hydrodynamic radius) was investigated under physiological conditions. In the presence of additional electrolytes, such as buffer, the critical micelle concentration decreased by a factor of about 10. In contrast, no influence of the probes on the critical micelle concentration and on average aggregation number was observed. The results show that HAF does not affect the characteristics of CTAB micelles. Analyzing fluorescence correlation spectroscopy data and time-resolved anisotropy decays in terms of the "two-step" in combination with the "wobbling-in-cone" model, it was proven that HAF and NIR 628 are differently associated with the micelles. Based on ensemble and single molecule fluorescence experiments, intra- and intermicellar energy transfer process between the two dyes were probed and characterized.
KW  - Hexadecyltrimethylammonium bromide
KW  - 5-Hexadecanoylaminofluorescein
KW  - 2,10-Bis-(3-aminopropyloxy)dibenzo[aj]perylene-8,16-dione
KW  - Fluorescence correlation spectroscopy
KW  - Fluorescence anisotropy
KW  - Single-molecule FRET
Y1  - 2012
U6  - https://doi.org/10.1016/j.jcis.2012.03.063
SN  - 0021-9797
VL  - 377
SP  - 251
EP  - 261
PB  - Elsevier
CY  - San Diego
ER  -