TY  - JOUR
A1  - Kienzler, Andrea Altevogt Nee
A1  - Flehr, Roman
A1  - Gehne, Sören
A1  - Kumke, Michael Uwe
A1  - Bannwarth, Willi
T1  - Verification and biophysical characterization of a New Three-Color Forster Resonance-Energy-Transfer (FRET) System in DNA
JF  - Helvetica chimica acta
N2  - We report on a new three-color FRET system consisting of three fluorescent dyes, i.e., of a carbostyril (=quinolin-2(1H)-one)-derived donor D, a (bathophenanthroline)ruthenium complex as a relay chromophore A1, and a Cy dye as A2 (FRET=Forster resonance-energy-transfer) (cf. Fig. 1). With their widely matching spectroscopic properties (cf. Fig. 2), the combination of these dyes yielded excellent FRET efficiencies. Furthermore, fluorescence lifetime measurements revealed that the long fluorescence lifetime of the Ru complex was transferred to the Cy dye offering the possibility to measure the whole system in a time-resolved mode. The FRET system was established on double-stranded DNA (cf. Fig. 3) but it should also be generally applicable to other biomolecules.
KW  - Forster resonance energy transfer (FRET) system
KW  - DNA
KW  - Fluorescence
KW  - Ruthenium complexes
Y1  - 2012
U6  - https://doi.org/10.1002/hlca.201100460
SN  - 0018-019X
VL  - 95
IS  - 4
SP  - 543
EP  - 555
PB  - Wiley-VCH
CY  - Weinheim
ER  -