TY  - JOUR
A1  - Rausch, Ann-Kristin
A1  - Brockmeyer, Robert
A1  - Schwerdtle, Tanja
T1  - Development and Validation of a QuEChERS-Based Liquid Chromatography Tandem Mass Spectrometry Multi-Method for the Determination of 38 Native and Modified Mycotoxins in Cereals
JF  - Journal of Agricultural and Food Chemistry
N2  - Here, a reliable and sensitive method for the determination of 38 (modified) mycotoxins was developed. Using a QuEChERS-based extraction method [acetonitrile/water/formic acid (75:20:5, v/v/v)], followed by two runs of high performance liquid chromatography tandem mass spectrometry with different conditions, relevant mycotoxins in cereals were analyzed. The method was validated according to the performance criteria defined by the European Commission (EC) in Commission Decision no. 657/2002. Limits of quantification ranged from 0.05 to 150 μg/kg. Good linearity (R2 > 0.99), recovery (61–120%), repeatability (RSDr < 15%), and reproducibility (RSDR < 20%) were obtained for most mycotoxins. However, validation results for Alternaria toxins and fumonisins were unsatisfying. Matrix effects (−69 to +59%) were compensated for using standard addition. Application on reference materials gave correct results while analysis of samples from local retailers revealed contamination, especially with deoxynivalenol, deoxynivalenol-3-glucoside, fumonisins, and zearalenone, in concentrations up to 369, 58, 1002, and 21 μg/kg, respectively.
KW  - multi-mycotoxin analysis
KW  - modified mycotoxins
KW  - QuEChERS
KW  - LC−MS/MS
KW  - cereals
KW  - validation
Y1  - 2020
U6  - https://doi.org/10.1021/acs.jafc.9b07491
SN  - 0021-8561
VL  - 68
IS  - 16
SP  - 4657
EP  - 4669
PB  - ACS Publications
CY  - Washington, DC
ER  -