TY  - JOUR
A1  - Kochovski, Zdravko
A1  - Chen, Guosong
A1  - Yuan, Jiayin
A1  - Lu, Yan
T1  - Cryo-Electron microscopy for the study of self-assembled poly(ionic liquid) nanoparticles and protein supramolecular structures
JF  - Colloid and polymer science : official journal of the Kolloid-Gesellschaft
N2  - Cryo-electron microscopy (cryo-EM) is a powerful structure determination technique that is well-suited to the study of protein and polymer self-assembly in solution. In contrast to conventional transmission electron microscopy (TEM) sample preparation, which often times involves drying and staining, the frozen-hydrated sample preparation allows the specimens to be kept and imaged in a state closest to their native one. Here, we give a short overview of the basic principles of Cryo-EM and review our results on applying it to the study of different protein and polymer self-assembled nanostructures. More specifically, we show how we have applied cryo-electron tomography (cryo-ET) to visualize the internal morphology of self-assembled poly(ionic liquid) nanoparticles and cryo-EM single particle analysis (SPA) to determine the three-dimensional (3D) structures of artificial protein microtubules.
KW  - self-assembly
KW  - poly(ionic liquid) nanoparticles
KW  - protein self-assembly
KW  - cryo-electron microscopy
KW  - single particle analysis
KW  - cryo-electron
KW  - tomography
Y1  - 2020
U6  - https://doi.org/10.1007/s00396-020-04657-w
SN  - 0303-402X
SN  - 1435-1536
VL  - 298
IS  - 7
SP  - 707
EP  - 717
PB  - Springer
CY  - New York
ER  -