TY  - JOUR
A1  - Neffe, Axel T.
A1  - von Rüsten-Lange, Maik
A1  - Braune, Steffen
A1  - Lützow, Karola
A1  - Roch, Toralf
A1  - Richau, Klaus
A1  - Jung, Friedrich
A1  - Lendlein, Andreas
T1  - Poly(ethylene glycol) grafting to Poly(ether imide) membranes - influence on protein adsorption and Thrombocyte adhesion
JF  - Macromolecular bioscience
N2  - The chain length and end groups of linear PEG grafted on smooth surfaces is known to influence protein adsorption and thrombocyte adhesion. Here, it is explored whether established structure function relationships can be transferred to application relevant, rough surfaces. Functionalization of poly(ether imide) (PEI) membranes by grafting with monoamino PEG of different chain lengths (M-n=1kDa or 10kDa) and end groups (methoxy or hydroxyl) is proven by spectroscopy, changes of surface hydrophilicity, and surface shielding effects. The surface functionalization does lead to reduction of adsorption of BSA, but not of fibrinogen. The thrombocyte adhesion is increased compared to untreated PEI surfaces. Conclusively, rough instead of smooth polymer or gold surfaces should be investigated as relevant models.
KW  - biomaterials
KW  - poly(ethylene glycol)
KW  - protein adsorption
KW  - surface functionalization
KW  - thrombocyte adhesion
Y1  - 2013
U6  - https://doi.org/10.1002/mabi.201300309
SN  - 1616-5187
SN  - 1616-5195
VL  - 13
IS  - 12
SP  - 1720
EP  - 1729
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Koshkina, Olga
A1  - Westmeier, Dana
A1  - Lang, Thomas
A1  - Bantz, Christoph
A1  - Hahlbrock, Angelina
A1  - Würth, Christian
A1  - Resch-Genger, Ute
A1  - Braun, Ulrike
A1  - Thiermann, Raphael
A1  - Weise, Christoph
A1  - Eravci, Murat
A1  - Mohr, Benjamin
A1  - Schlaad, Helmut
A1  - Stauber, Roland H.
A1  - Docter, Dominic
A1  - Bertin, Annabelle
A1  - Maskos, Michael
T1  - Tuning the Surface of Nanoparticles: Impact of Poly(2-ethyl-2-oxazoline) on Protein Adsorption in Serum and Cellular Uptake
JF  - Macromolecular bioscience
N2  - Due to the adsorption of biomolecules, the control of the biodistribution of nanoparticles is still one of the major challenges of nanomedicine. Poly(2-ethyl-2-oxazoline) (PEtOx) for surface modification of nanoparticles is applied and both protein adsorption and cellular uptake of PEtOxylated nanoparticles versus nanoparticles coated with poly(ethylene glycol) (PEG) and non-coated positively and negatively charged nanoparticles are compared. Therefore, fluorescent poly(organosiloxane) nanoparticles of 15 nm radius are synthesized, which are used as a scaffold for surface modification in a grafting onto approach. With multi-angle dynamic light scattering, asymmetrical flow field-flow fractionation, gel electrophoresis, and liquid chromatography-mass spectrometry, it is demonstrated that protein adsorption on PEtOxylated nanoparticles is extremely low, similar as on PEGylated nanoparticles. Moreover, quantitative microscopy reveals that PEtOxylation significantly reduces the non-specific cellular uptake, particularly by macrophage-like cells. Collectively, studies demonstrate that PEtOx is a very effective alternative to PEG for stealth modification of the surface of nanoparticles.
KW  - cellular uptake
KW  - nanoparticles
KW  - poly(2-ethyl-2oxazoline)
KW  - poly(ethylene glycol)
KW  - protein adsorption
Y1  - 2016
U6  - https://doi.org/10.1002/mabi.201600074
SN  - 1616-5187
SN  - 1616-5195
VL  - 16
SP  - 1287
EP  - 1300
PB  - Wiley-VCH
CY  - Weinheim
ER  -