TY  - JOUR
A1  - Warncke, Max
A1  - Vogt, Birgit
A1  - Ulrich, Jacqueline
A1  - von Laer, Meike Dorothee
A1  - Beyer, Winfried
A1  - Klump, Hannes
A1  - Micheel, Burkhard
A1  - Sheriff, Ahmed
T1  - Efficient in vitro transduction of naive murine B cells with lentiviral vectors
N2  - The aim of this study was to determine the impact of lentiviral transduction on primary murine B cells. Studying B cell activities in vivo or using them for tolerance induction requires that the cells remain unaltered in their biological behavior except for expression of the transgene. As we show here, murine B cells can efficiently be transduced by lentiviral, VSV-G-pseudotyped vectors without the necessity of prior activation. Culture with LPS gave enhanced transduction efficiencies but led to the upregulation of CD86 and proliferation of the cells. Transduction of naive B cells by lentiviral vectors was dependent on multiplicity of infection and did not lead to a concomitant activation. Furthermore, the transduced cells could be used for studies in the NOD mouse system without altering the onset of diabetes. We conclude that lentiviral gene transfer into naive B cells is a powerful tool for manipulation of B cells for therapeutic applications.
Y1  - 2004
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4C707VR- 7&_coverDate=06%2F04%2F2004&_alid=269000954&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6713&_sort=d&view=c&_acct=C000053886&_v e
ER  -