TY  - JOUR
A1  - Zizola, C. F.
A1  - Frey, Simone K.
A1  - Jitngarmkusol, S.
A1  - Kadereit, Bert
A1  - Yan, N.
A1  - Vogel, Silke
T1  - Cellular retinol-binding protein type I (CRBP-I) regulates adipogenesis
N2  - Adipogenesis is governed by a well-documented cascade of transcription factors. However, less is known about non-transcription factors that govern early stages of adipogenesis. Here we show that cellular retinol-binding protein type I (CRBP-I), a small cytosolic binding protein for retinol and retinaldehyde, is specifically restricted to preadipocytes in white adipose tissue. The absence of CRBP-I in mice (CRBP-I-KO mice) leads to increased adiposity. Despite increased adiposity, CRBP-I-KO mice remain more glucose tolerant and insulin sensitive during high-fat-diet feeding. 3T3-L1 cells deficient in CRBP-I or mouse embryonic fibroblasts derived from CRBP-I-KO mice had increased adipocyte differentiation and triglyceride (TG) accumulation. This was due to increased expression and activity of PPAR gamma, while other transcription factor pathways in early and late differentiation remained unchanged. Conversely, the overexpression of CRBP-I in 3T3-L1 cells results in decreased TG accumulation. In conclusion, CRBP-I is a cytosolic protein specifically expressed in preadipocytes that regulates adipocyte differentiation in part by affecting PPAR gamma activity.
Y1  - 2010
UR  - http://mcb.asm.org/
U6  - https://doi.org/10.1128/Mcb.00014-10
SN  - 0270-7306
ER  - 
TY  - JOUR
A1  - Henze, Andrea
A1  - Frey, Simone K.
A1  - Raila, Jens
A1  - Scholze, Alexandra
A1  - Spranger, Joachim
A1  - Weickert, Martin O.
A1  - Tepel, Martin
A1  - Zidek, Walter
A1  - Schweigert, Florian J.
T1  - Alterations of retinol-binding protein 4 species in patients with different stages of chronic kidney disease and their relation to lipid parameters
N2  - Retinol-binding protein 4 (RBP4) is elevated in patients with chronic kidney disease (CKD) and has been discussed as marker of kidney function. In addition to an elevated concentration, the existence of truncated RBP4 species, RBP4-L (truncated at last C-terminal leucine) and RBP4-LL (truncated at both C-terminal leucines), has been reported in serum of hemodialysis patients. Since little is known about the occurrence of RBP4 species during the progression of CKD it was the aim of this study to analyse this possible association. The presence of RBP4, RBP4-L, RBP4- LL and transthyretin (TTR) was assessed in serum of 45 healthy controls and 52 patients with stage 2-5 of CKD using ELISA and RBP4 immunoprecipitation with subsequent MALDI-TOF-MS analysis. A reduction of glomerular filtration rate was accompanied by a gradual elevation of RBP4 serum levels and relative amounts of RBP4-LL. Correlation analysis revealed a strong association of the RBP4-TTR ratio with parameters of lipid metabolism and with diabetes-related factors. In conclusion, RBP4 serum concentration and the appearance of RBP4-LL seem to be influenced by kidney function. Furthermore, the RBP4-TTR ratio may provide diagnostic potential with regard to metabolic complications in CKD patients.
Y1  - 2010
UR  - http://www.sciencedirect.com/science/journal/0006291X
U6  - https://doi.org/10.1016/j.bbrc.2010.01.082
SN  - 0006-291X
ER  -