TY - JOUR A1 - Einarsson, Jon M. A1 - Bahrke, Sven A1 - Sigurdsson, Bjarni Thor A1 - Ng, Chuen-How A1 - Petersen, Petur Henry A1 - Sigurjonsson, Olafur E. A1 - Jonsson, Halldor A1 - Gislason, Johannes A1 - Thormodsson, Finnbogi R. A1 - Peter, Martin G. T1 - Partially acetylated chitooligosaccharides bind to YKL-40 and stimulate growth of human osteoarthritic chondrocytes JF - Biochemical and biophysical research communications N2 - Recent evidences indicating that cellular kinase signaling cascades are triggered by oligomers of N-acetylglucosamine (ChOS) and that condrocytes of human osteoarthritic cartilage secrete the inflammation associated chitolectin YKL-40, prompted us to study the binding affinity of partially acetylated ChOS to YKL-40 and their effect on primary chondrocytes in culture. Extensive chitinase digestion and filtration of partially deacetylated chitin yielded a mixture of ChOS (Oligomin(TM)) and further ultrafiltration produced T-ChOS(TM), with substantially smaller fraction of the smallest sugars. YKL-40 binding affinity was determined for the different sized homologues, revealing micromolar affinities of the larger homologues to YKL-40. The response of osteoarthritic chondrocytes to Oligomin(TM) and T-ChOS(TM) was determined, revealing 2- to 3-fold increases in cell number. About 500 mu g/ml was needed for Oligomin(TM) and around five times lower concentration for T-ChOS(TM), higher concentrations abolished this effect for both products. Addition of chitotriose inhibited cellular responses mediated by larger oligosaccharides. These results, and the fact that the partially acetylated T-ChOS(TM) homologues should resist hydrolysis, point towards a new therapeutic concept for treating inflammatory joint diseases. KW - Cell culture KW - Chitolectin KW - Chitooligosaccharides KW - Chondrocytes KW - High affinity binding KW - Rheumatoid arthritis KW - YKL-40 Y1 - 2013 U6 - https://doi.org/10.1016/j.bbrc.2013.02.122 SN - 0006-291X VL - 434 IS - 2 SP - 298 EP - 304 PB - Elsevier CY - San Diego ER - TY - JOUR A1 - Haebel, Sophie A1 - Bahrke, Sven A1 - Peter, Martin G. T1 - Quantitative sequencing of complex mixtures of heterochitooligosaccharides by vMALDI-linear ion trap mass spectrometry N2 - Heterochitooligosaccharides possess interesting biol. properties. Isobaric mixts. of such linear heterochitooligosaccharides can be obtained by chem. or enzymic degrdn. of chitosan. However, the sepn. of such mixts. is a challenging anal. problem which is so far unresolved. It is shown that these isobaric mixts. can be sequenced and quantified simultaneously using std. derivatization and multistage tandem mass spectrometric techniques. A linear ion trap mass spectrometer equipped with a vacuum matrix-assisted laser desorption ionization (vMALDI) source is used to perform MS2 as well as MS3 expts. [on SciFinder (R)]. Y1 - 2007 UR - http://pubs.acs.org/loi/ancham U6 - https://doi.org/10.1021/Ac062254u SN - 0003-2700 ER - TY - JOUR A1 - Cederkvist, F. Henning A1 - Zamfir, Alina D. A1 - Bahrke, Sven A1 - Eijsink, Vincent G. H. A1 - Sorlie, Morten A1 - Peter-Katalinic, Jasna A1 - Peter, Martin G. T1 - Identification of a high-affinity-binding oligosaccharide by (+) nanoelectrospray quadrupole time-of-flight tandem mass spectrometry of a noncovalent enzyme-ligand complex Y1 - 2006 UR - http://www3.interscience.wiley.com/cgi-bin/jhome/26737/ U6 - https://doi.org/10.1002/anie.200503168 SN - 1433-7851 ER - TY - JOUR A1 - Bahrke, Sven A1 - Einarsson, Jon M. A1 - Gislason, Johannes A1 - Haebel, Sophie A1 - Letzel, Matthias C. A1 - Peter-Katalinic, Jasna A1 - Peter, Martin G. T1 - Sequence analysis of chitooligosaccharides by matrix-assisted laser desorption ionization postsource decay mass spectrometry N2 - Oligosaccharides composed of 2-acetamido-2-deoxy-D-glucopyranose (GlcNAc) and/or 2-amino-2-deoxy-D- glucopyranose (GlcN) were prepd. by chem. degrdn. of chitin or chitosan and sepd. by gel permeation chromatog. Oligosaccharides obtained after enzymic hydrolysis of chitosan [FA 0.19] with a fungal chitinase were derivatized by reductive amination with 2-aminoacridone and sequenced by matrix-assisted laser desorption ionization time-of-flight postsource decay (PSD) mass spectrometry (MS). The sequence of a trimer, D1A2, was established as D-A-A. The compn. of a hexamer D3A3 was .apprx.65% D-A-D-D-A-A and 35% D-D-A-D-A-A. The PSD MS of a nonamer D5A4-amac revealed four isobaric species D-X-Y-D-X-Y-D-A-A, where A is GlcNAc, D is GlcN, and X and Y (X ยน Y) are mutually either D or A. This structure motif was also obsd. in a dodecamer D7A5 which was composed of eight isobaric sequences of the general formula (D-X-Y)3- D-A-A. Y1 - 2002 ER - TY - THES A1 - Bahrke, Sven A1 - Einarsson, Jon M. A1 - Gislason, Johannes A1 - Haebel, Sophie A1 - Peter-Katalinic, Jasna A1 - Peter, Martin G. T1 - Characterization of chitooligosaccharides by mass spectrometry Y1 - 2003 SN - 82-47-15901-5 ER - TY - JOUR A1 - Bahrke, Sven A1 - Einarsson, Jon M. A1 - Gislason, Johannes A1 - Haebel, Sophie A1 - Peter-Katalinic, Jasna T1 - Characterization of chitooligosaccharides by mass spectrometry N2 - Heterochitooligosaccharides of DP 6, DP 9, and DP 12 were evaluated using established methods of derivatization and matrix-assisted laser desorption ionization post source decay mass spectrometry. Y1 - 2003 SN - 82-471-5901-5 ER -