TY  - GEN
A1  - Dunsing, Valentin
A1  - Magnus, Mayer
A1  - Liebsch, Filip
A1  - Multhaup, Gerhard
A1  - Chiantia, Salvatore
T1  - Direct Evidence of APLP1 Trans Interactions in Cell-Cell Adhesion Platforms Investigated via Fluorescence Fluctuation Spectroscopy
T2  - Biophysical journal
N2  - The Amyloid-precursor-like protein 1 (APLP1) is a neuronal type I transmembrane protein which plays a role in synaptic adhesion and synaptogenesis. Past investigations indicated that APLP1 is involved in the formation of protein-protein complexes that bridge the junctions between neighboring cells. Nevertheless, APLP1-APLP1 trans interactions have never been directly observed in higher eukaryotic cells. Here, we investigate APLP1 interactions and dynamics directly in living human embryonic kidney (HEK) cells, using fluorescence fluctuation spectroscopy techniques, namely cross-correlation scanning fluorescence correlation spectroscopy (sFCS) and Number&Brightness (N&B). Our results show that APLP1 forms homotypic trans complexes at cell-cell contacts. In the presence of zinc ions, the protein forms macroscopic clusters, exhibiting an even higher degree of trans binding and strongly reduced dynamics. Further evidence from Giant Plasma Membrane Vesicles and live cell actin staining suggests that the presence of an intact cortical cytoskeleton is required for zinc-induced cis multimerization. Subsequently, large adhesion platforms bridging interacting cells are formed through APLP1-APLP1 direct trans interactions. Taken together, our results provide direct evidence that APLP1 functions as a neuronal zinc-dependent adhesion protein and provide a more detailed understanding of the molecular mechanisms driving the formation of APLP1 adhesion platforms. Further, they show that fluorescence fluctuation spectroscopy techniques are useful tools for the investigation of protein-protein interactions at cell-cell adhesion sites.
Y1  - 2018
U6  - https://doi.org/10.1016/j.bpj.2017.11.2067
SN  - 0006-3495
SN  - 1542-0086
VL  - 114
IS  - 3
SP  - 373A
EP  - 373A
PB  - Cell Press
CY  - Cambridge
ER  - 
TY  - GEN
A1  - Luckner, Madlen
A1  - Dunsing, Valentin
A1  - Chiantia, Salvatore
A1  - Hermann, Andreas
T1  - Oligomerization and nuclear shuttling dynamics of viral proteins studied by quantitative molecular brightness analysis using fluorescence correlation spectroscopy
T2  - Biophysical journal
Y1  - 2018
U6  - https://doi.org/10.1016/j.bpj.2017.11.1951
SN  - 0006-3495
SN  - 1542-0086
VL  - 114
IS  - 3
SP  - 350A
EP  - 350A
PB  - Cell Press
CY  - Cambridge
ER  - 
TY  - GEN
A1  - Luckner, Madlen
A1  - Dunsing, Valentin
A1  - Drüke, Markus
A1  - Zuehlke, B.
A1  - Petazzi, Roberto Arturo
A1  - Chiantia, Salvatore
A1  - Herrmann, A.
T1  - Quantifying protein oligomerization directly in living cells
BT  - a systematic comparison of fluorescent proteins and application to Influenza A virus infection
T2  - European biophysics journal : with biophysics letters ; an international journal of biophysics
Y1  - 2019
SN  - 0175-7571
SN  - 1432-1017
VL  - 48
SP  - S183
EP  - S183
PB  - Springer
CY  - New York
ER  - 
TY  - GEN
A1  - Dunsing, Valentin
A1  - Irmscher, Tobias
A1  - Barbirz, Stefanie
A1  - Chiantia, Salvatore
T1  - Microviscosity of bacterial biofilm matrix characterized by fluorescence correlation spectroscopy and single particle tracking
T2  - European biophysics journal : with biophysics letters ; an international journal of biophysics
Y1  - 2019
U6  - https://doi.org/https://doi.org/10.1007/s00249-019-01373-4
SN  - 0175-7571
SN  - 1432-1017
VL  - 48
SP  - S115
EP  - S115
PB  - Springer
CY  - New York
ER  - 
TY  - GEN
A1  - Höfer, Chris Tina
A1  - Di Lella, Santiago
A1  - Dahmani, Ismail
A1  - Jungnick, Nadine
A1  - Bordag, Natalie
A1  - Bobone, Sara
A1  - Huan, Q.
A1  - Keller, S.
A1  - Herrmann, A.
A1  - Chiantia, Salvatore
T1  - Corrigendum to: Structural determinants of the interaction between influenza A virus matrix protein M1 and lipid membranes (Biochimica et Biophysica Acta (BBA) - Biomembranes. - 1861, (2019), pg 1123-1134)
T2  - Biochimica et biophysica acta : Biomembranes
Y1  - 2019
U6  - https://doi.org/10.1016/j.bbamem.2019.07.002
SN  - 0005-2736
SN  - 1879-2642
VL  - 1861
IS  - 10
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - GEN
A1  - Luckner, Madlen
A1  - Dunsing, Valentin
A1  - Chiantia, Salvatore
A1  - Herrmann, Andreas
T1  - Influenza virus vRNPs: quantitative investigations via fluorescence
cross-correlation spectroscopy
T2  - European biophysics journal : with biophysics letters ; an international journal of biophysics
Y1  - 2017
SN  - 0175-7571
SN  - 1432-1017
VL  - 46
SP  - S368
EP  - S368
PB  - Springer
CY  - New York
ER  - 
TY  - GEN
A1  - Dunsing, Valentin
A1  - Mayer, M.
A1  - Multhaup, G.
A1  - Chiantia, Salvatore
T1  - Direct visualization of APLP1 cell-cell adhesion platforms via fluorescence fluctuation spectroscopy
T2  - European biophysics journal : with biophysics letters ; an international journal of biophysics
Y1  - 2017
SN  - 0175-7571
SN  - 1432-1017
VL  - 46
SP  - S374
EP  - S374
PB  - Springer
CY  - New York
ER  -