TY  - JOUR
A1  - Yarman, Aysu
A1  - Schulz, Christopher
A1  - Sygmund, Cristoph
A1  - Ludwig, Roland
A1  - Gorton, Lo
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Third generation ATP sensor with enzymatic analyte recycling
JF  - Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis
N2  - For the first time the direct electron transfer of an enzyme - cellobiose dehydrogenase, CDH - has been coupled with the hexokinase catalyzed competition for glucose in a sensor for ATP. To enhance the signal output for ATP, pyruvate kinase was coimmobilized to recycle ADP by the phosphoenolpyruvate driven reaction. The new sensor overcomes the limit of 1:1 stoichiometry of the sequential or competitive conversion of ATP by effective enzymatic recycling of the analyte. The anodic oxidation of the glucose converting CDH proceeds at electrode potentials below 0 mV vs. Ag vertical bar AgCl thus potentially interfering substances like ascorbic acid or catecholamines do not influence the measuring signal. The combination of direct electron transfer of CDH with the enzymatic recycling results in an interference-free and oxygen-independent measurement of ATP in the lower mu molar concentration range with a lower limit of detection of 63.3 nM (S/N=3).
KW  - ATP
KW  - Third generation sensor
KW  - Enzymatic recycling
KW  - Cellobiose dehydrogenase
KW  - Hexokinase
KW  - Pyruvate kinase
Y1  - 2014
U6  - https://doi.org/10.1002/elan.201400231
SN  - 1040-0397
SN  - 1521-4109
VL  - 26
IS  - 9
SP  - 2043
EP  - 2048
PB  - Wiley-VCH
CY  - Weinheim
ER  -