TY  - JOUR
A1  - Elias-Miro, Maria
A1  - Massip-Salcedo, Marta
A1  - Raila, Jens
A1  - Schweigert, Florian J.
A1  - Mendes-Braz, Mariana
A1  - Ramalho, Fernando
A1  - Jimenez-Castro, Monica B.
A1  - Casillas-Ramirez, Arani
A1  - Bermudo, Raquel
A1  - Rimola, Antoni
A1  - Rodes, Juan
A1  - Peralta, Carmen
T1  - Retinol binding protein 4 and retinol in steatotic and nonsteatotic rat livers in the setting of partial hepatectomy under ischemia/reperfusion
JF  - Liver transplantation
N2  - Steatotic livers show increased hepatic damage and impaired regeneration after partial hepatectomy (PH) under ischemia/reperfusion (I/R), which is commonly applied in clinical practice to reduce bleeding. The known function of retinol-binding protein 4 (RBP4) is to transport retinol in the circulation. We examined whether modulating RBP4 and/or retinol could protect steatotic and nonsteatotic livers in the setting of PH under I/R. Steatotic and nonsteatotic livers from Zucker rats were subjected to PH (70%) with 60 minutes of ischemia. RBP4 and retinol levels were measured and altered pharmacologically, and their effects on hepatic damage and regeneration were studied after reperfusion. Decreased RBP4 levels were observed in both liver types, whereas retinol levels were reduced only in steatotic livers. RBP4 administration exacerbated the negative consequences of liver surgery with respect to damage and liver regeneration in both liver types. RBP4 affected the mobilization of retinol from steatotic livers, and this revealed actions of RBP4 independent of simple retinol transport. The injurious effects of RBP4 were not due to changes in retinol levels. Treatment with retinol was effective only for steatotic livers. Indeed, retinol increased hepatic injury and impaired liver regeneration in nonsteatotic livers. In steatotic livers, retinol reduced damage and improved regeneration after surgery. These benefits of retinol were associated with a reduced accumulation of hepatocellular fat. Thus, strategies based on modulating RBP4 could be ineffective and possibly even harmful in both liver types in the setting of PH under I/R. In terms of clinical applications, a retinol pretreatment might open new avenues for liver surgery that specifically benefit the steatotic liver. Liver Transpl 18:1198-1208, 2012.
Y1  - 2012
U6  - https://doi.org/10.1002/lt.23489
SN  - 1527-6465
VL  - 18
IS  - 10
SP  - 1198
EP  - 1208
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -