TY  - JOUR
A1  - Lu, Dandan
A1  - Wang, Ting
A1  - Persson, Staffan
A1  - Müller-Röber, Bernd
A1  - Schippers, Jos H. M.
T1  - Transcriptional control of ROS homeostasis by KUODA1 regulates cell expansion during leaf development
JF  - Nature Communications
N2  - The final size of an organism, or of single organs within an organism, depends on an intricate coordination of cell proliferation and cell expansion. Although organism size is of fundamental importance, the molecular and genetic mechanisms that control it remain far from understood. Here we identify a transcription factor, KUODA1 (KUA1), which specifically controls cell expansion during leaf development in Arabidopsis thaliana. We show that KUA1 expression is circadian regulated and depends on an intact clock. Furthermore, KUA1 directly represses the expression of a set of genes encoding for peroxidases that control reactive oxygen species (ROS) homeostasis in the apoplast. Disruption of KUA1 results in increased peroxidase activity and smaller leaf cells. Chemical or genetic interference with the ROS balance or peroxidase activity affects cell size in a manner consistent with the identified KUA1 function. Thus, KUA1 modulates leaf cell expansion and final organ size by controlling ROS homeostasis.
Y1  - 2014
U6  - https://doi.org/10.1038/ncomms4767
SN  - 2041-1723
VL  - 5
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Fujikura, Ushio
A1  - Elsaesser, Lore
A1  - Breuninger, Holger
A1  - Sanchez-Rodriguez, Clara
A1  - Ivakov, Alexander
A1  - Laux, Thomas
A1  - Findlay, Kim
A1  - Persson, Staffan
A1  - Lenhard, Michael
T1  - Atkinesin-13A modulates cell-wall synthesis and cell expansion in arabidopsis thaliana via the THESEUS1 pathway
JF  - PLoS Genetics : a peer-reviewed, open-access journal
N2  - Growth of plant organs relies on cell proliferation and expansion. While an increasingly detailed picture about the control of cell proliferation is emerging, our knowledge about the control of cell expansion remains more limited. We demonstrate the internal-motor kinesin AtKINESIN-13A (AtKIN13A) limits cell expansion and cell size in Arabidopsis thaliana, ion atkinl3a mutants forming larger petals with larger cells. The homolog, AtKINESIN-13B, also affects cell expansion and double mutants display growth, gametophytic and early embryonic defects, indicating a redundant role of he two genes. AtKIN13A is known to depolymerize microtubules and influence Golgi motility and distribution. Consistent his function, AtKIN13A interacts genetically with ANGUSTIFOLIA, encoding a regulator of Golgi dynamics. Reduced AtIGN13A activity alters cell wall structure as assessed by Fourier-transformed infrared-spectroscopy and triggers signalling he THESEUS1-dependent cell-wall integrity pathway, which in turn promotes the excess cell expansion in the atkinl3a mutant. Thus, our results indicate that the intracellular activity of AtKIN13A regulates cell expansion and wall architecture via THESEUS1, providing a compelling case of interplay between cell wall integrity sensing and expansion.
Y1  - 2014
U6  - https://doi.org/10.1371/journal.pgen.1004627
SN  - 1553-7390
SN  - 1553-7404
VL  - 10
IS  - 9
PB  - PLoS
CY  - San Fransisco
ER  -