TY  - JOUR
A1  - Uhlig, Katja
A1  - Wischerhoff, Erik
A1  - Lutz, Jean-Francois
A1  - Laschewsky, André
A1  - Jäger, Magnus S.
A1  - Lankenau, Andreas
A1  - Duschl, Claus
T1  - Monitoring cell detachment on PEG-based thermoresponsive surfaces using TIRF microscopy
N2  - Recently, we introduced a thermoresponsive copolymer that consists of oligo(ethylene glycol) methacrylate (OEGMA) and 2-(2- methoxyethoxy) ethyl methacrylate (MEO(2)MA). The polymer exhibited an LCST at 35 degrees C in PBS buffer and was anchored onto gold substrates using disulfide polymerisation initiators. It allows the noninvasive detachment of adherent cells from their substrate. As the mechanisms that determine the interaction of cells with such polymers are not well understood, we employed Total Internal Reflection Fluorescence (TIRF) microscopy in order to monitor the detachment process of cells of two different types. We identified contact area and average cell-substrate distance as crucial parameters for the evaluation of the detachment process. The sensitivity of TIRF microscopy allowed us to correlate the specific adhesion pattern of MCF-7 breast cancer cells with the morphology of cell deposits that may serve as fingerprints for a nondestructive characterisation of live cells.
Y1  - 2010
UR  - http://www.rsc.org/Publishing/Journals/sm/index.asp
U6  - https://doi.org/10.1039/C0sm00010h
SN  - 1744-683X
ER  - 
TY  - JOUR
A1  - Wischerhoff, Erik
A1  - Glatzel, Stefan
A1  - Uhlig, Katja
A1  - Lankenau, Andreas
A1  - Lutz, Jean-Francois
A1  - Laschewsky, André
T1  - Tuning the thickness of polymer brushes grafted from nonlinearly growing multilayer assemblies
N2  - A new versatile method for tuning the thickness of surface-tethered polymer brushes is introduced. It is based on the combination of polyelectrolyte multilayer deposition and surface-initiated atom transfer radical polymerization. To control the thickness of the brushes, the nonlinear growth of certain polyelectrolyte multilayer systems is exploited. The method is demonstrated to work with different polyelectrolytes and different monomers. The relevance for applications is demonstrated by cell adhesion experiments oil grafted thermoresponsive polymer layers with varying thickness.
Y1  - 2009
UR  - http://pubs.acs.org/journal/langd5
U6  - https://doi.org/10.1021/La804197j
SN  - 0743-7463
ER  -